Human APE1 (APEX1) activation kit by CRISPRa

CAT#: GA100229

APEX1 CRISPRa kit - CRISPR gene activation of human apurinic/apyrimidinic endodeoxyribonuclease 1

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: APEX1
• Locus ID: 328
• Kit Components:

  GA100229G1, APE1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100229G2, APE1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100229G3, APE1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001244249, NM_001641, NM_080648, NM_080649
• UniProt ID: P27695
• Synonyms: APE; APE1; APEN; APEX; APX; HAP1; REF1
• Summary: The APEX gene encodes the major AP endonuclease in human cells. It encodes the APEX endonuclease, a DNA repair enzyme with apurinic/apyrimidinic (AP) activity. Such AP activity sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. The AP sites are the most frequent pre-mutagenic lesions that can prevent normal DNA replication. Splice variants have been found for this gene; all encode the same protein. Disruptions in the biological functions related to APEX are associated with many various malignancies and neurodegenerative diseases.[provided by RefSeq, Dec 2019]