P2RY5 (LPAR6) (NM_005767) Human Mutant ORF Clone

CAT#: RC402791

LPAR6 Mutant (D63V), Myc-DDK-tagged ORF clone of Homo sapiens lysophosphatidic acid receptor 6 (LPAR6), transcript variant 1 as transfection-ready DNA

Specifications

Product Data

• Mutation Description: D63V
• Affected Codon#: 63
• Affected NT#: 188
• Tag: Myc-DDK
• Effect: Woolly hir
• Symbol: LPAR6
• Synonyms: ARWH1; HYPT8; LAH3; LPA-6; P2RY5; P2Y5
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Vector: pCMV6-Entry
• ACCN: NM_005767
• ORF Size: 1032 bp
• Sequence Data:
  ORF Nucleotide Sequence

  >RC402791 representing NM_005767
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAAGCGTTAACAGCTCCCACTGCTTCTATAATGACTCCTTTAAGTACACTTTGTATGGGTGCATGT
  TCAGCATGGTGTTTGTGCTTGGGTTAATATCCAATTGTGTTGCCATATACATTTTCATCTGCGTCCTCAA
  AGTCCGAAATGAAACTACAACTTACATGATTAACTTGGCAATGTCAGTCTTGCTTTTTGTTTTTACTTTA
  CCCTTCAGGATTTTTTACTTCACAACACGGAATTGGCCATTTGGAGATTTACTTTGTAAGATTTCTGTGA
  TGCTGTTTTATACCAACATGTACGGAAGCATTCTGTTCTTAACCTGTATTAGTGTAGATCGATTTCTGGC
  AATTGTCTACCCATTTAAGTCAAAGACTCTAAGAACCAAAAGAAATGCAAAGATTGTTTGCACTGGCGTG
  TGGTTAACTGTGATCGGAGGAAGTGCACCCGCCGTTTTTGTTCAGTCTACCCACTCTCAGGGTAACAATG
  CCTCAGAAGCCTGCTTTGAAAATTTTCCAGAAGCCACATGGAAAACATATCTCTCAAGGATTGTAATTTT
  CATCGAAATAGTGGGATTTTTTATTCCTCTAATTTTAAATGTAACTTGTTCTAGTATGGTGCTAAAAACT
  TTAACCAAACCTGTTACATTAAGTAGAAGCAAAATAAACAAAACTAAGGTTTTAAAAATGATTTTTGTAC
  ATTTGATCATATTCTGTTTCTGTTTTGTTCCTTACAATATCAATCTTATTTTATATTCTCTTGTGAGAAC
  ACAAACATTTGTTAATTGCTCAGTAGTGGCAGCAGTAAGGACAATGTACCCAATCACTCTCTGTATTGCT
  GTTTCCAACTGTTGTTTTGACCCTATAGTTTACTACTTTACATCGGACACAATTCAGAATTCAATAAAAA
  TGAAAAACTGGTCTGTCAGGAGAAGTGACTTCAGATTCTCTGAAGTTCATGGTGCAGAGAATTTTATTCA
  GCATAACCTACAGACCTTAAAAAGTAAGATATTTGACAATGAATCTGCTGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGA TAAGGTTTAA
  Protein Sequence

  >RC402791 representing NM_005767
  Red=Cloning site Green=Tags(s)
  
  MVSVNSSHCFYNDSFKYTLYGCMFSMVFVLGLISNCVAIYIFICVLKVRNETTTYMINLAMSVLLFVFTL
  PFRIFYFTTRNWPFGDLLCKISVMLFYTNMYGSILFLTCISVDRFLAIVYPFKSKTLRTKRNAKIVCTGV
  WLTVIGGSAPAVFVQSTHSQGNNASEACFENFPEATWKTYLSRIVIFIEIVGFFIPLILNVTCSSMVLKT
  LTKPVTLSRSKINKTKVLKMIFVHLIIFCFCFVPYNINLILYSLVRTQTFVNCSVVAAVRTMYPITLCIA
  VSNCCFDPIVYYFTSDTIQNSIKMKNWSVRRSDFRFSEVHGAENFIQHNLQTLKSKIFDNESAA
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NP_005758
• RefSeq Size: 1032 bp
• RefSeq ORF: 1035 bp
• Locus ID: 10161
• Cytogenetics: 13q14.2
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 37.8 kDa
• Gene Summary: The protein encoded by this gene belongs to the family of G-protein coupled receptors, that are preferentially activated by adenosine and uridine nucleotides. This gene aligns with an internal intron of the retinoblastoma susceptibility gene in the reverse orientation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]