HA (NC_007374) Virus Tagged ORF Clone

CAT#: VC102447

Myc-DDK-tagged ORF clone of viral ORF for hemagglutinin [Influenza A virus (A/Korea/426/68(H2N2))], codon optimized for human cell expression, YP_308850

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102447 represents NCBI reference of YP_308850 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTATCATTTACCTCATCCTGCTGTTTACCGCCGTAAGAGGTGACCAGATCTGCATCGGCTATCACG
  CGAACAACAGTACCGAAAAGGTGGACACTATATTGGAGCGAAACGTGACCGTGACACATGCTAAAGATAT
  CCTGGAGAAAACCCATAATGGGAAATTGTGTAAGCTCAATGGTATCCCCCCCCTCGAATTGGGGGACTGT
  AGCATCGCCGGCTGGCTCCTGGGGAACCCTGAATGTGACAGGCTCCTCTCCGTGCCTGAGTGGAGCTATA
  TCATGGAGAAGGAAAATCCCAGGTACTCTCTTTGCTACCCCGGTTCATTTAACGATTATGAAGAACTTAA
  GCACCTTCTTAGCTCTGTGAAGCACTTTGAAAAGGTAAAGATTCTGCCAAAAGACCGGTGGACACAGCAT
  ACTACTACGGGAGGCTCCTGGGCGTGCGCTGTCAGCGGCAAGCCCAGCTTCTTTCGCAATATGGTGTGGC
  TGACAAGAAAAGGGTCAAATTACCCTGTGGCCAAGGGCTCTTATAATAACACCTCTGGGGAACAGATGTT
  GATCATCTGGGGAGTGCATCACCCTAATGACGAGGCCGAGCAGAGAGCCTTGTACCAAAATGTAGGCACA
  TATGTATCAGTTGCTACCTCAACTCTCTACAAGAGGAGTATCCCCGAGATTGCTGCAAGGCCTAAAGTGA
  ATGGCCTCGGCCGCAGGATGGAATTTAGTTGGACCTTGTTGGATATGTGGGACACAATTAACTTTGAGTC
  AACTGGCAATCTGGTGGCACCGGAATACGGCTTCAAGATCTCAAAGCGGGGTTCCTCAGGCATCATGAAG
  ACGGAAGGTACTCTCGAAAACTGTGAGACCAAGTGCCAAACTCCTCTGGGAGCGATAAATACCACCCTCC
  CGTTCCACAATGTGCACCCCCTCACAATTGGGGAATGTCCCAAGTACGTGAAATCCGAAAAACTTGTCCT
  GGCTACCGGCCTCAGGAACGTGCCCCAGATCGAAAGCAGGGGTTTGTTCGGAGCGATTGCAGGGTTTATT
  GAGGGCGGCTGGCAGGGAATGGTCGACGGCTGGTATGGATACCATCATAGCAATGACCAGGGTAGTGGCT
  ATGCTGCCGATAAAGAGAGCACCCAGAAGGCCTTTAACGGAATCACAAACAAAGTCAATTCAGTGATCGA
  AAAGATGAACACACAATTCGAAGCCGTGGGCAAAGAGTTTTCAAACCTCGAGAAACGCCTGGAGAACCTC
  AACAAGAAGATGGAAGATGGATTCCTCGACGTATGGACCTATAATGCGGAACTCCTTGTACTGATGGAGA
  ACGAAAGGACGCTGGACTTCCATGACTCCAATGTCAAAAACTTGTACGACAAAGTTAGAATGCAGCTGCG
  CGACAATGTGAAGGAGCTGGGCAATGGGTGCTTTGAATTCTACCACAAGTGTGATAATGAGTGTATGGAC
  TCAGTCAAAAATGGAACATATGACTACCCCAAATATGAGGAAGAGAGCAAGTTGAATAGGAACGAGATTA
  AAGGGGTCAAGTTGTCCTCAATGGGGGTGTACCAGATTCTGGCCATCTACGCAACTGTTGCAGGAAGCCT
  CAGTCTGGCTATCATGATGGCAGGAATCAGCTTCTGGATGTGCTCTAACGGCTCACTCCAGTGCAGGATT
  TGCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102447 representing YP_308850
  Red=Cloning sites Green=Tags
  
  MAIIYLILLFTAVRGDQICIGYHANNSTEKVDTILERNVTVTHAKDILEKTHNGKLCKLNGIPPLELGDC
  SIAGWLLGNPECDRLLSVPEWSYIMEKENPRYSLCYPGSFNDYEELKHLLSSVKHFEKVKILPKDRWTQH
  TTTGGSWACAVSGKPSFFRNMVWLTRKGSNYPVAKGSYNNTSGEQMLIIWGVHHPNDEAEQRALYQNVGT
  YVSVATSTLYKRSIPEIAARPKVNGLGRRMEFSWTLLDMWDTINFESTGNLVAPEYGFKISKRGSSGIMK
  TEGTLENCETKCQTPLGAINTTLPFHNVHPLTIGECPKYVKSEKLVLATGLRNVPQIESRGLFGAIAGFI
  EGGWQGMVDGWYGYHHSNDQGSGYAADKESTQKAFNGITNKVNSVIEKMNTQFEAVGKEFSNLEKRLENL
  NKKMEDGFLDVWTYNAELLVLMENERTLDFHDSNVKNLYDKVRMQLRDNVKELGNGCFEFYHKCDNECMD
  SVKNGTYDYPKYEEESKLNRNEIKGVKLSSMGVYQILAIYATVAGSLSLAIMMAGISFWMCSNGSLQCRI
  CI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007374
• ORF Size: 1686 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007374.1, YP_308850
• RefSeq ORF: 1686 bp
• Locus ID: 3655103
• MW: 63.3 kDa