hypothetical protein (NC_002190) Virus Tagged ORF Clone

CAT#: VC102275

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein Ihahnvgp1 [Infectious hypodermal and hematopoietic necrosis virus], codon optimized for human cell expression, YP_003572960

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: hypothetical protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102275 represents NCBI reference of YP_003572960 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAACAGATTCCGTGAACACAGTTATTCCCACTACCAAGAATGGCCAAAAGATCTGGCCTCGCACAT
  ACTGCATTCATGTTAGGGCTAACCAGAATCATTTGGTGAACTGCTTTGAGAATGCACGCACCAAGCTCAC
  GCCAGCGAGAGAATTTTTGCAAGCTTTTTCACCCCAGGTCCAGATTAAGACCCTGAACCCTTTGCCTAAT
  AACTTTTTGATTTGTCTTAAGAACTGCAGCAGCTTCCAAACCCGCAAAACGACTCTGAAGCTGCTCCAGG
  AACTGCAGCACCAGAACAAACTGATCCTGCGGTCCGCATGGATCACATGGGAGTTCGAAAGGGAACAGGA
  GACAGAGCAGTTCAACCTTGAAGTCAACCAAAAGCCCCCCCTGGAAGTTCTGGAGACAGTAATGACCGGA
  GTGAAGCGGGACCGCGAAGAACTGTTGACAAGTGTCACACACCAGATTCCACCCGACCAAATGACGAAGA
  TCTGGGTCCATCACATCGAGATAAAGGAGCCTGAGACACCTACCCCACAGAAACGCCAGGCTGAGGAGAC
  CACCGACGATATTAGGAAAGCCATCATGGAGGCTATGGAAATAGACTGGAACTTGCCCATGGAGGAGCGA
  GCCGTCGTGGCTGAACATAACCCCACCCTCCTGAAGGGGCTGCCGACAGACCGAACTAAATGGACAGAGG
  GAGACTGGAAGAGGGTGAGACTTATCAATAAATGGAAGGTGCAACATGGCACCCCTAGCTCCTCCGAGAA
  GAACCATAATCAAGAGGACTCTCCAGATGAGCGGCAGACAAGTCCACTCCAAATCATGGTAACCACAGGA
  ACATCTCACACACCCGATACACAACCAATTAGACCCGATATCGAGCTTCAGTCAAGCCCAATATGGGAGC
  TTCCCTTGCTGCCCGAACCTAAGCTTAAACGCCTCCGCTATTTGTTGGAAATCAGCCGCGACGGGTACTC
  AATACTGTCTGACACCGTACTGAACGGCTTTCGGATTCTGGTTCTGGCTACTCCTTTCCTGAATGAATCA
  TCTAACACTTCTAATAATACTGAGGAGACCAAGACCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102275 representing YP_003572960
  Red=Cloning sites Green=Tags
  
  MSTDSVNTVIPTTKNGQKIWPRTYCIHVRANQNHLVNCFENARTKLTPAREFLQAFSPQVQIKTLNPLPN
  NFLICLKNCSSFQTRKTTLKLLQELQHQNKLILRSAWITWEFEREQETEQFNLEVNQKPPLEVLETVMTG
  VKRDREELLTSVTHQIPPDQMTKIWVHHIEIKEPETPTPQKRQAEETTDDIRKAIMEAMEIDWNLPMEER
  AVVAEHNPTLLKGLPTDRTKWTEGDWKRVRLINKWKVQHGTPSSSEKNHNQEDSPDERQTSPLQIMVTTG
  TSHTPDTQPIRPDIELQSSPIWELPLLPEPKLKRLRYLLEISRDGYSILSDTVLNGFRILVLATPFLNES
  SNTSNNTEETKTQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_002190
• ORF Size: 1089 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_002190.2, YP_003572960
• RefSeq ORF: 1089 bp
• Locus ID: 1457863
• MW: 42.1 kDa