E6 (NC_014952) Virus Tagged ORF Clone

CAT#: VC101860

Myc-DDK-tagged ORF clone of viral ORF for E6 [Human papillomavirus type 128], codon optimized for human cell expression, YP_004169263

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101860 represents NCBI reference of YP_004169263 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAGTCTCCAGCCCACCCGACTCAACGAGTACTGTAAGCTGCACTCCATTGATTTTTTTGACCTTC
  GGCTCAGATGCATCTTCTGTCAGTTCATATGTTCCCTCGAAGATCTTGCCTCATTTTTTGAGAAAAATCT
  CTCTCTCGTGTACAGGAACAACCTGCCATTTGCCTGTTGCATGAAGTGTCTGAAGCAGACCGCTATTTAT
  GAACGAGAGAGATTCTGTCAGTGCACAGTCAAATCATCTATCATTGATGTTGTAACAGGAAAGTCACTGC
  AGGACCTGATTGTTCGGTGTCTGTACTGTTTTGCTCTGCTTGACTCAATCGAGAAAAGGGAATGCCTGAA
  CCGAGATGAGGATTTGTGCCTGGTTCGAGGCCACTGGCGGTGGTATTGTAGGAACTGCTTCAGCCTTCAT
  TACGAGGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101860 representing YP_004169263
  Red=Cloning sites Green=Tags
  
  MASLQPTRLNEYCKLHSIDFFDLRLRCIFCQFICSLEDLASFFEKNLSLVYRNNLPFACCMKCLKQTAIY
  ERERFCQCTVKSSIIDVVTGKSLQDLIVRCLYCFALLDSIEKRECLNRDEDLCLVRGHWRWYCRNCFSLH
  YEG
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014952
• ORF Size: 429 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_014952.1, YP_004169263
• RefSeq ORF: 429 bp
• Locus ID: 10146726
• MW: 17.0 kDa