HCoV-NL63 ORF3 Gene Tagged ORF Clone
CAT#: VC100594
- TrueORF®
Myc-DDK-tagged ORF clone for protein 3 [Human coronavirus NL63], codon optimized for human cell expression, YP_003768
View other clones from "HCoV-NL63" (4)
Product Images
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | protein 3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC100594 represents NCBI reference of YP_003768 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTTTGGAGGTCTGTTTCAGCTCACCCTGGAAAGTACTATTAACAAAAGCGTGGCCAACCTCAAGC TCCCACCTCACGACGTGACAGTGCTGAGAGATAACCTGAAGCCTGTCACTACTCTGTCTACCATCACCGC CTACCTGTTGGTGTCCCTTTTCGTTACCTACTTTGCATTGTTCAAGCCTCTGACCGCAAGAGGCAGGGTT GCATGTTTCGTCCTGAAACTCCTCACATTGTTTGTGTATGTTCCACTGCTGGTGCTGTTCGGGATGTATC TGGACAGTTTCATCATTTTCTCCACCCTCCTGTTCCGCTTTATCCACGTGGGCTATTATGCCTACCTGTA CAAAAACTTTTCCTTCGTCTTGTTTAACGTGACGAAACTCTGTTTCGTGTCAGGCAAGTGTTGGTATCTT GAGCAGTCCTTCTACGAAAATAGGTTCGCTGCCATATATGGGGGGGATCACTACGTAGTGCTGGGCGGCG AGACCATCACCTTTGTCAGCTTTGACGACCTGTACGTGGCCATTCGCGGCTCATGCGAGAAGAATCTTCA ACTGATGCGAAAAGTGGACCTGTATAACGGAGCCGTAATTTACATTTTCGCCGAAGAACCCGTCGTGGGA ATTGTCTACTCTTCACAACTGTACGAGGATGTACCCAGTATTAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100594 representing YP_003768
Red=Cloning sites Green=Tags MPFGGLFQLTLESTINKSVANLKLPPHDVTVLRDNLKPVTTLSTITAYLLVSLFVTYFALFKPLTARGRV ACFVLKLLTLFVYVPLLVLFGMYLDSFIIFSTLLFRFIHVGYYAYLYKNFSFVLFNVTKLCFVSGKCWYL EQSFYENRFAAIYGGDHYVVLGGETITFVSFDDLYVAIRGSCEKNLQLMRKVDLYNGAVIYIFAEEPVVG IVYSSQLYEDVPSIN TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NC_005831 |
ORF Size | 675 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NC_005831.2, YP_003768 |
RefSeq ORF | 675 bp |
Locus ID | 2943500 |
MW | 25.7 kDa |
Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
VC100593 | Myc-DDK-tagged ORF clone for spike protein [Human coronavirus NL63], codon optimized for human cell expression, YP_003767 |
USD 1,160.00 |
|
VC100595 | Myc-DDK-tagged ORF clone for envelope protein [Human coronavirus NL63], codon optimized for human cell expression, YP_003769 |
USD 150.00 |
|
VC100596 | Myc-DDK-tagged ORF clone for membrane protein [Human coronavirus NL63], codon optimized for human cell expression, YP_003770 |
USD 300.00 |
|
VC100597 | Myc-DDK-tagged ORF clone for nucleocapsid protein [Human coronavirus NL63], codon optimized for human cell expression, YP_003771 |
USD 686.00 |
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