L4 (NC_003266) Virus Tagged ORF Clone

CAT#: VC100479

Myc-DDK-tagged ORF clone of viral ORF for hexon assembly protein 100K [Human adenovirus E], codon optimized for human cell expression, YP_068035

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100479 represents NCBI reference of YP_068035 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACGCAGCCATCTTCACCTACGTCCCCCTCAGCCCCAACCGCAGATAAGAAACAGCAGCAGAACG
  AGAGTCTCACAGCTCCACCCCCCTCCCCAGCCACGTTCGTCGCAGCCCCAGACATGCAGGAGATGGAAGA
  GAGTATCCAGATTGATTTGGGATATGTTACCCCCGCGGAGCACGAAGAGGAGCTGGCAGTAAGGTTTTCT
  ACCCAAGAGGAGATCCATCAGGAACAACCTGAGCAAGAAGCCAAGTCTGAACACGATTATCTTCACCAAA
  GCGGAGGGGAGGATGCACTGATCAAACACCTGGCCCGGCAGGCAATTATCGTCAAAGATGCACTCCTGGA
  TAGGACCGAGGTGCCGCTCAGCGTGGAAGAACTGAGTCGCGCCTATGAACTGAATCTGTTTTCACCCAGG
  GTGCCTCCCAAGCGACAACCAAATGGCACCTGCGAACCAAACCCACGGCTGAATTTTTACCCCGTCTTCG
  CAGTGCCGGAGGCACTCGCCACTTACCACATCTTCTTTAAGAACCAACGAATTCCTGTGAGCTGTCGGGC
  GAACAGAACTAGAGCAGATTCATTGTTTAACCTGGGACCTGGAGCTAGACTGCCAGACATCGCTAGTTTG
  GAAGAAGTGCCAAAGATCTTTGAAGGATTGGGCTCAGACGAGACGCGCGCAGCAAACGCACTGCAGGGTG
  AAGGGGGCGACCATGAGCACCACTCTGCTCTCGTAGAGCTCGAAGGCGATAACGCCCGACTGGCTGTTCT
  GAAGCGGACCATTGAACTCACCCATTTTGCTTACCCAGCCCTGAACCTGCCACCAAAGGTTATGTCAACC
  GTGATGGACCAGGTGCTCATTAAGCGAGCCTCACCTATTAGCAAGGAAATGCAGGACCCAGAGTCCTCAG
  AAGAGGGGAAACCTGTGGTCTCCGACGAGCAACTGGCAAGATGGCTTGGACCACAAGCCAGTCCCCAATC
  TCTGGAGGAACGGCGAAAATTGATTATGGCTGTCGTGCTGGTGACTGCCGAACTTGAGTGCCTTAGGCGA
  TTCTTCGCCGATGCCGAGATCCTGCGCAAGGTTGAGGAAAATTTGCACTATATATTTAGACACGGTTTTG
  TCCGGCAGGCTTGCAAAATCAGTAACGTCGAGCTTACCAACCTCGTTAGCTACATGGGGATACTGCACGA
  GAACCGACTGGGGCAAAACGTGCTTCACACTACCCTGAGGGGAGAGGCTAGGCGGGATTACATCAGGGAC
  TGCGTTTATCTTTACCTTTGCCATACGTGGCAAACTGCTATGGGCGTGTGGCAACAGTGCCTGGAGGAGC
  AGAATCTGAAGGAATTGTGTAAACTTCTCCAAAAGAATCTGAAAGCCCTGTGGACTGGATTTGATGAGCG
  AACTACCGCCTCCGACCTGGCAGACCTCATCTTTCCCGAGAGACTGCGACTGACCCTGCGAAACGGGCTT
  CCTGACTTCATGTCCCAGAGTATGCTGCAGAATTTCCGGTCTTTTATCCTGGAGCGCTCAGGAATACTGC
  CAGCCACCTGCTCCGCACTCCCTAGTGATTTTGTGCCACTGACTTTTCGGGAATGTCCCCCTCCTTTGTG
  GTCTCACTGCTACCTCCTCAGACTCGCAAACTATCTTGCGTATCACTCAGACGTGATTGAGGACGTCAGT
  AGCGAGGGCCTGCTGGAGTGTCATTGCCGCTGTAATCTCTGTACACCCCACAGGTCCCTGGCATGCAATC
  CTCAGCTCTTGTCTGAGACACAGATCATCGGAACATTTGAACTCCAGGGACCAGGCGACGAGGGCAGCGC
  GGCTAAGGGCGGACTGAAGCTTACACCTGGGTTGTGGACCTCCGCCTACCTGCGCAAATTCGTACCAGAA
  GACTACCACCCCTTTGAAATCAGATTTTATGAAGACCAATCCCAGCCACCAAAGGCTGAGCTTAGCGCCT
  GTGTGATCACACAGGGAGCGATTCTTGCCCAACTCCAGGCCATCCAGAAGTCACGGCAGGAGTTCCTCTT
  GAAAAAAGGCCGAGGAGTGTACCTCGACCCTCAGACGGGCGAGGAACTGAATCCGGGCTTTCCACAGGAT
  GCGCCCCGGAAGCAACAGGAAGCTGAAAGTGGTGCAGCCGCTAGAGGGGGTTTTGGTGGAAGGCTTGGAG
  AGCAATCCGGAAGAGGGGGAGGTGATGGAAGACTGGGTCAACACTCAGGTCGCGGCCAACCTGCCCGGCA
  GTCTGGAAGAAGGGGGGGGGGTAGAGGGGGTGGGAGGTCCAGCCGCCGCAGGCAGACAGTGGTACTGGGT
  GGTGAGTCTAAACAGCACGGTTACCACCTGCGGTCAGGGTCTGGCAGTCGGTCCACTCCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100479 representing YP_068035
  Red=Cloning sites Green=Tags
  
  METQPSSPTSPSAPTADKKQQQNESLTAPPPSPATFVAAPDMQEMEESIQIDLGYVTPAEHEEELAVRFS
  TQEEIHQEQPEQEAKSEHDYLHQSGGEDALIKHLARQAIIVKDALLDRTEVPLSVEELSRAYELNLFSPR
  VPPKRQPNGTCEPNPRLNFYPVFAVPEALATYHIFFKNQRIPVSCRANRTRADSLFNLGPGARLPDIASL
  EEVPKIFEGLGSDETRAANALQGEGGDHEHHSALVELEGDNARLAVLKRTIELTHFAYPALNLPPKVMST
  VMDQVLIKRASPISKEMQDPESSEEGKPVVSDEQLARWLGPQASPQSLEERRKLIMAVVLVTAELECLRR
  FFADAEILRKVEENLHYIFRHGFVRQACKISNVELTNLVSYMGILHENRLGQNVLHTTLRGEARRDYIRD
  CVYLYLCHTWQTAMGVWQQCLEEQNLKELCKLLQKNLKALWTGFDERTTASDLADLIFPERLRLTLRNGL
  PDFMSQSMLQNFRSFILERSGILPATCSALPSDFVPLTFRECPPPLWSHCYLLRLANYLAYHSDVIEDVS
  SEGLLECHCRCNLCTPHRSLACNPQLLSETQIIGTFELQGPGDEGSAAKGGLKLTPGLWTSAYLRKFVPE
  DYHPFEIRFYEDQSQPPKAELSACVITQGAILAQLQAIQKSRQEFLLKKGRGVYLDPQTGEELNPGFPQD
  APRKQQEAESGAAARGGFGGRLGEQSGRGGGDGRLGQHSGRGQPARQSGRRGGGRGGGRSSRRRQTVVLG
  GESKQHGYHLRSGSGSRSTPQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003266
• ORF Size: 2373 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_003266.2, YP_068035
• RefSeq ORF: 2373 bp
• Locus ID: 2958479
• MW: 88.1 kDa