L1 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100433

Myc-DDK-tagged ORF clone of viral ORF for capsid protein precursor pIIIa [Human adenovirus D], codon optimized for human cell expression, YP_001974449

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100433 represents NCBI reference of YP_001974449 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCAAGCTCCCGATCCAGCCATACGGGCAGCCCTCCAATCCCAGCCCAGTGGGTTGGCTTCAGATG
  ACTGGGAGGCTGCCATGCAGAGGATCATGGCATTGACTACACGGAACCCCGAAAGCTTCAGGCAGCAACC
  GCAGGCTAACAGACTCTCAGCCATCCTTGAGGCAGTGGTACCTAGCCGCACGAACCCCACTCACGAGAAG
  GTGCTCGCCATCGTAAATGCCCTGGCCGAGAACAAGGCCATCAGACCGGACGAGGCAGGCCTCGTGTATA
  ATGCCCTGCTCGAGAGAGTAGGGAGATACAACAGTACAAACGTCCAGAGCAACCTCGACAGATTGGTAAC
  TGACGTGAGAGAAGCAGTAGCGCAACGGGAGCGATTCAAGAACGAGGGTCTTGGTTCCCTGGTAGCACTG
  AACGCCTTCCTTGCAACCCAGCCAGCCAACGTGCCCCGGGGGCAAGATGATTACACCAACTTCATTTCAG
  CCTTGCGGCTGATGGTAACCGAGGTGCCCCAGTCTGAGGTATATCAAAGCGGCCCAGATTATTTCTTCCA
  GACTTCACGCCAGGGCCTTCAGACAGTGAATCTCTCACAGGCATTCAAGAACTTGAGGGGTCTGTGGGGG
  GTACAGGCCCCTGTCGGGGACCGCTCTACGGTGTCTTCTCTCCTGACACCCAATTCAAGGCTGCTCCTGC
  TCCTGATTGCACCATTTACCGACTCCGGAAGTGTTAATCGCAACTCTTATTTGGGCCATCTGCTTACACT
  GTATAGAGAGGCAATCGGCCAGGCACAGGTAGACGAGCAGACGTTTCAGGAGATAACTAGCGTTAGCAGA
  GCACTGGGTCAAAACGATACCGATTCCCTGCGCGCAACACTGAATTTCCTGCTGACCAACCGGCAACAGA
  AAATTCCTGCGCAATATGCTCTTTCCGCCGAAGAAGAACGGATCCTTAGATACGTGCAGCAGTCAGTGGG
  TCTGTTCCTGATGCAGGAAGGGGCTACACCTTCAGCCGCCCTTGACATGACCGCAAGAAATATGGAGCCC
  TCCATGTACGCAGCTAACCGACCTTTCATTAACAAGCTGATGGACTACTTGCATAGAGCTGCTGTGATGA
  ACACGGACTACTTTACAAACGCGATCCTCAACCCCCACTGGCTGCCTCCACCAGGATTTTACACTGGAGA
  ATATGATATGCCGGACCCCAATGATGGATTTCTTTGGGACGACGTCGACAGTGTGGTGTTCTCCCCGACC
  TTTCAAAAGAGACAGGAAGCTCCCCCCTCCGAGGGTGCCGTCGGTAGAAGCCCATTCCCTTCCCTCGGGA
  GCCTGCACTCTCTGCCTGGATCCGTTAACAGCGGAAGAGTTTCCCGGCCCCGATTGCTCGGAGAGGACGA
  GTATCTTAACGACTCTCTTCTCCAGCCTCCGAGGGCTAAGAACGCTATGGCGAATAATGGAATCGAATCA
  CTGGTCGATAAGCTGAACAGGTGGAAGACGTACGCCCAAGACCACAGGGACGCACCGGCCCCAAGGCGCC
  AGCGCCATGATCGCCAGAGAGGACTGGTTTGGGACGATGAGGATAGCGCTGATGATAGTTCCGTTTTGGA
  TTTGGGAGGGTCAGGCGGTGTAAATCCTTTCGCTCACCTCCAGCCGAAGCTGGGCAGGCGAATGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100433 representing YP_001974449
  Red=Cloning sites Green=Tags
  
  MQQAPDPAIRAALQSQPSGLASDDWEAAMQRIMALTTRNPESFRQQPQANRLSAILEAVVPSRTNPTHEK
  VLAIVNALAENKAIRPDEAGLVYNALLERVGRYNSTNVQSNLDRLVTDVREAVAQRERFKNEGLGSLVAL
  NAFLATQPANVPRGQDDYTNFISALRLMVTEVPQSEVYQSGPDYFFQTSRQGLQTVNLSQAFKNLRGLWG
  VQAPVGDRSTVSSLLTPNSRLLLLLIAPFTDSGSVNRNSYLGHLLTLYREAIGQAQVDEQTFQEITSVSR
  ALGQNDTDSLRATLNFLLTNRQQKIPAQYALSAEEERILRYVQQSVGLFLMQEGATPSAALDMTARNMEP
  SMYAANRPFINKLMDYLHRAAVMNTDYFTNAILNPHWLPPPGFYTGEYDMPDPNDGFLWDDVDSVVFSPT
  FQKRQEAPPSEGAVGRSPFPSLGSLHSLPGSVNSGRVSRPRLLGEDEYLNDSLLQPPRAKNAMANNGIES
  LVDKLNRWKTYAQDHRDAPAPRRQRHDRQRGLVWDDEDSADDSSVLDLGGSGGVNPFAHLQPKLGRRMF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 1677 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_010956.1, YP_001974449
• RefSeq ORF: 1677 bp
• Locus ID: 6386276
• MW: 62.1 kDa