L2 (NC_011203) Virus Tagged ORF Clone

CAT#: VC100338

Myc-DDK-tagged ORF clone of viral ORF for penton base [Human adenovirus B], codon optimized for human cell expression, YP_002213774

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100338 represents NCBI reference of YP_002213774 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCCGGAGAGCCGTGCTTGGGGGCGCAGTGGTGTATCCCGAAGGTCCTCCTCCGAGCTACGAATCAG
  TCATGCAGCAACAGGCGGCCATGATTCAACCTCCCCTGGAGGCACCTTTCGTACCCCCACGATATCTGGC
  TCCGACAGAGGGCAGAAACTCTATCAGATACAGCGAGCTCTCTCCTCTTTACGATACGACCAAGCTCTAC
  CTGGTGGATAATAAGTCTGCCGACATCGCTTCTCTTAATTACCAGAACGATCACTCTAATTTCCTGACCA
  CAGTAGTGCAGAACAATGATTTCACTCCAACCGAGGCCTCTACACAGACTATCAACTTCGACGAGAGATC
  AAGATGGGGAGGTCAGCTCAAAACCATCATGCATACCAATATGCCGAATGTTAACGAATACATGTTTAGT
  AACAAGTTCAAGGCTAGGGTGATGGTGTCCCGAAAGGCTCCAGAGGGCGTGACAGTAAACGATACCTATG
  ATCATAAGGAGGATATTCTCAAGTACGAATGGTTCGAATTCATACTGCCAGAAGGGAATTTTAGCGCTAC
  AATGACTATTGACCTTATGAATAACGCAATCATTGACAACTATCTTGAAATCGGTAGGCAGAACGGTGTT
  CTCGAAAGCGACATTGGAGTGAAATTTGATACGAGGAATTTTAGACTCGGGTGGGATCCAGAAACCAAGT
  TGATAATGCCAGGAGTATATACATATGAGGCATTCCACCCAGACATTGTGCTCCTGCCCGGCTGCGGTGT
  TGACTTCACAGAAAGTCGCCTGAGCAATTTGCTGGGTATTAGGAAACGCCATCCTTTCCAAGAGGGCTTT
  AAGATAATGTACGAAGATCTTGAAGGAGGGAATATTCCAGCGCTCCTCGACGTTACCGCCTACGAGGAAT
  CTAAAAAGGATACCACCACAGAGACAACCACACTGGCAGTGGCGGAAGAGACATCCGAGGATGACGACAT
  CACCCGAGGAGACACATATATCACAGAAAAGCAGAAGCGGGAGGCTGCCGCCGCCGAAGTGAAGAAGGAG
  CTCAAAATACAGCCTTTGGAGAAAGACAGTAAGAGCCGAAGTTACAACGTGCTCGAGGATAAGATCAACA
  CGGCTTATCGCTCCTGGTATCTGTCTTATAATTATGGTAATCCTGAGAAGGGTATCAGAAGTTGGACCCT
  TTTGACGACTTCTGACGTCACCTGCGGCGCCGAGCAAGTGTACTGGTCTCTTCCTGACATGATGCAGGAC
  CCGGTGACATTTCGGAGCACAAGGCAGGTTAACAACTACCCGGTCGTGGGAGCAGAACTCATGCCTGTGT
  TCTCTAAGTCTTTCTATAACGAACAGGCTGTGTACTCTCAGCAGCTCAGGCAGGCTACTTCACTGACACA
  CGTCTTTAACAGGTTCCCAGAAAACCAGATTCTCATCAGGCCCCCTGCCCCCACTATCACCACAGTGTCT
  GAGAATGTCCCTGCATTGACCGATCACGGTACCCTCCCCCTTAGGAGCTCTATCAGAGGGGTCCAGCGGG
  TGACCGTGACCGACGCTAGGAGGCGGACATGCCCCTATGTGTATAAAGCCCTGGGAATCGTCGCCCCGCG
  AGTTCTCAGTAGCAGGACCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100338 representing YP_002213774
  Red=Cloning sites Green=Tags
  
  MRRRAVLGGAVVYPEGPPPSYESVMQQQAAMIQPPLEAPFVPPRYLAPTEGRNSIRYSELSPLYDTTKLY
  LVDNKSADIASLNYQNDHSNFLTTVVQNNDFTPTEASTQTINFDERSRWGGQLKTIMHTNMPNVNEYMFS
  NKFKARVMVSRKAPEGVTVNDTYDHKEDILKYEWFEFILPEGNFSATMTIDLMNNAIIDNYLEIGRQNGV
  LESDIGVKFDTRNFRLGWDPETKLIMPGVYTYEAFHPDIVLLPGCGVDFTESRLSNLLGIRKRHPFQEGF
  KIMYEDLEGGNIPALLDVTAYEESKKDTTTETTTLAVAEETSEDDDITRGDTYITEKQKREAAAAEVKKE
  LKIQPLEKDSKSRSYNVLEDKINTAYRSWYLSYNYGNPEKGIRSWTLLTTSDVTCGAEQVYWSLPDMMQD
  PVTFRSTRQVNNYPVVGAELMPVFSKSFYNEQAVYSQQLRQATSLTHVFNRFPENQILIRPPAPTITTVS
  ENVPALTDHGTLPLRSSIRGVQRVTVTDARRRTCPYVYKALGIVAPRVLSSRTF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011203
• ORF Size: 1632 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_011203.1, YP_002213774
• RefSeq ORF: 1632 bp
• Locus ID: 6870522
• MW: 61.8 kDa