Metabotropic Glutamate Receptor 1 (GRM1) (NM_001278067) Human Untagged Clone

CAT#: SC337659

GRM1 (untagged) - Human glutamate receptor, metabotropic 1 (GRM1), transcript variant 6

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRM1
• Synonyms: GPRC1A; MGLU1; MGLUR1; PPP1R85; SCA44; SCAR13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001278067, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTCGGGCTCCTTTTGTTTTTTTTCCCAGCGATCTTTTTGGAGGTGTCCCTTCTCCCCAGAAGCCCCG
  GCAGGAAAGTGTTGCTGGCAGGAGCGTCGTCTCAGCGCTCGGTGGCCAGAATGGACGGAGATGTCATCAT
  TGGAGCCCTCTTCTCAGTCCATCACCAGCCTCCGGCCGAGAAAGTGCCCGAGAGGAAGTGTGGGGAGATC
  AGGGAGCAGTATGGCATCCAGAGGGTGGAGGCCATGTTCCACACGTTGGATAAGATCAACGCGGACCCGG
  TCCTCCTGCCCAACATCACCCTGGGCAGTGAGATCCGGGACTCCTGCTGGCACTCTTCCGTGGCTCTGGA
  ACAGAGCATTGAGTTCATTAGGGACTCTCTGATTTCCATTCGAGATGAGAAGGATGGGATCAACCGGTGT
  CTGCCTGACGGCCAGTCCCTCCCCCCAGGCAGGACTAAGAAGCCCATTGCGGGAGTGATCGGTCCCGGCT
  CCAGCTCTGTAGCCATTCAAGTGCAGAACCTGCTCCAGCTCTTCGACATCCCCCAGATCGCTTATTCAGC
  CACAAGCATCGACCTGAGTGACAAAACTTTGTACAAATACTTCCTGAGGGTTGTCCCTTCTGACACTTTG
  CAGGCAAGGGCCATGCTTGACATAGTCAAACGTTACAATTGGACCTATGTCTCTGCAGTCCACACGGAAG
  GGAATTATGGGGAGAGCGGAATGGACGCTTTCAAAGAGCTGGCTGCCCAGGAAGGCCTCTGTATCGCCCA
  TTCTGACAAAATCTACAGCAACGCTGGGGAGAAGAGCTTTGACCGACTCTTGCGCAAACTCCGAGAGAGG
  CTTCCCAAGGCTAGAGTGGTGGTCTGCTTCTGTGAAGGCATGACAGTGCGAGGACTCCTGAGCGCCATGC
  GGCGCCTTGGCGTCGTGGGCGAGTTCTCACTCATTGGAAGTGATGGATGGGCAGACAGAGATGAAGTCAT
  TGAAGGTTATGAGGTGGAAGCCAACGGGGGAATCACGATAAAGCTGCAGTCTCCAGAGGTCAGGTCATTT
  GATGATTATTTCCTGAAACTGAGGCTGGACACTAACACGAGGAATCCCTGGTTCCCTGAGTTCTGGCAAC
  ATCGGTTCCAGTGCCGCCTTCCAGGACACCTTCTGGAAAATCCCAACTTTAAACGAATCTGCACAGGCAA
  TGAAAGCTTAGAAGAAAACTATGTCCAGGACAGTAAGATGGGGTTTGTCATCAATGCCATCTATGCCATG
  GCACATGGGCTGCAGAACATGCACCATGCCCTCTGCCCTGGCCACGTGGGCCTCTGCGATGCCATGAAGC
  CCATCGACGGCAGCAAGCTGCTGGACTTCCTCATCAAGTCCTCATTCATTGGAGTATCTGGAGAGGAGGT
  GTGGTTTGATGAGAAAGGAGACGCTCCTGGAAGGTATGATATCATGAATCTGCAGTACACTGAAGCTAAT
  CGCTATGACTATGTGCACGTTGGAACCTGGCATGAAGGAGTGCTGAACATTGATGATTACAAAATCCAGA
  TGAACAAGAGTGGAGTGGTGCGGTCTGTGTGCAGTGAGCCTTGCTTAAAGGGCCAGATTAAGGTTATACG
  GAAAGGAGAAGTGAGCTGCTGCTGGATTTGCACGGCCTGCAAAGAGAATGAATATGTGCAAGATGAGTTC
  ACCTGCAAAGCTTGTGACTTGGGATGGTGGCCCAATGCAGATCTAACAGGCTGTGAGCCCATTCCTGTGC
  GCTATCTTGAGTGGAGCAACATCGAATCCATTATAGCCATCGCCTTTTCATGCCTGGGAATCCTTGTTAC
  CTTGTTTGTCACCCTAATCTTTGTACTGTACCGGGACACACCAGTGGTCAAATCCTCCAGTCGGGAGCTC
  TGCTACATCATCCTAGCTGGCATCTTCCTTGGTTATGTGTGCCCATTCACTCTCATTGCCAAACCTACTA
  CCACCTCCTGCTACCTCCAGCGCCTCTTGGTTGGCCTCTCCTCTGCGATGTGCTACTCTGCTTTAGTGAC
  TAAAACCAATCGTATTGCACGCATCCTGGCTGGCAGCAAGAAGAAGATCTGCACCCGGAAGCCCAGGTTC
  ATGAGTGCCTGGGCTCAGGTGATCATTGCCTCAATTCTGATTAGTGTGCAACTAACCCTGGTGGTAACCC
  TGATCATCATGGAACCCCCTATGCCCATTCTGTCCTACCCAAGTATCAAGGAAGTCTACCTTATCTGCAA
  TACCAGCAACCTGGGTGTGGTGGCCCCTTTGGGCTACAATGGACTCCTCATCATGAGCTGTACCTACTAT
  GCCTTCAAGACCCGCAACGTGCCCGCCAACTTCAACGAGGCCAAATATATCGCGTTCACCATGTACACCA
  CCTGTATCATCTGGCTAGCTTTTGTGCCCATTTACTTTGGGAGCAACTACAAGATCATCACAACTTGCTT
  TGCAGTGAGTCTCAGTGTAACAGTGGCTCTGGGGTGCATGTTCACTCCCAAGATGTACATCATTATTGCC
  AAGCCTGAGAGGAATGTCCGCAGTGCCTTCACCACCTCTGATGTTGTCCGCATGCATGTTGGCGATGGCA
  AGCTGCCCTGCCGCTCCAACACTTTCCTCAACATCTTCCGAAGAAAGAAGGCAGGGGCAGGGAATGCCAA
  GTGGAGGACAGGTGCCCAAGGGACAGCATATGTGGCACCGCCTCTCTGTGCACGTGAAGACCAATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001278067
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278067.1, NP_001264996.1
• RefSeq Size: 6672 bp
• RefSeq ORF: 2727 bp
• Locus ID: 2911
• UniProt ID: Q13255
• Cytogenetics: 6q24.3
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Gap junction, Long-term depression, Long-term potentiation, Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes a metabotropic glutamate receptor that functions by activating phospholipase C. L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The canonical alpha isoform of the encoded protein is a disulfide-linked homodimer whose activity is mediated by a G-protein-coupled phosphatidylinositol-calcium second messenger system. This gene may be associated with many disease states, including schizophrenia, bipolar disorder, depression, and breast cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]
  Transcript Variant: This variant (6) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 3. The encoded isoform (d) is shorter and contains a distinct C-terminus, compared to isoform alpha. Isoform d may have a distinct function compared to isoform alpha (PMID: 9776379).