PFKP (NM_001242339) Human Untagged Clone

CAT#: SC337434

PFKP (untagged) - Human phosphofructokinase, platelet (PFKP), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PFKP
• Synonyms: ATP-PFK; PFK-C; PFK-P; PFKF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001242339, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGCGGGTATGAACGCTGCCGTCCGTGCCGTGGTGCGCATGGGTATCTACGTGGGGGCCAAGGTGTAC
  TTCATCTACGAGGGCTACCAGGGCATGGTGGACGGAGGCTCAAACATCGCAGAGGCCGACTGGGAGAGTG
  TCTCCAGCATCCTGCAAGTGGTGCTGTAAGGGGTGACTGGAGGGAGAAGCCTGGCTGCTGGTCACACCGC
  TTCCCTTGTCCTGGCCGGCATGCTCTGGTGGGCGGGACGATCATTGGCAGTGCGCGGTGCCAGGCCTTCC
  GCACGCGGGAAGGCCGCCTGAAGGCTGCTTGCAACCTGCTGCAGCGCGGCATCACCAACCTGTGTGTGAT
  CGGCGGGGACGGGAGCCTCACCGGGGCCAACCTCTTCCGGAAGGAGTGGAGTGGGCTGCTGGAGGAGCTG
  GCCAGGAACGGCCAGATCGATAAGGAGGCCGTGCAGAAGTACGCCTACCTCAACGTGGTGGGCATGGTGG
  GCTCCATCGACAATGATTTCTGCGGCACCGACATGACCATCGGCACGGACTCCGCCCTGCACAGGATCAT
  CGAGGTCGTCGACGCCATCATGACCACGGCCCAGAGCCACCAGAGGACCTTCGTTCTGGAGGTGATGGGA
  CGACACTGTGGGTACCTGGCCCTGGTGAGTGCCTTGGCCTGCGGTGCGGACTGGGTGTTCCTTCCAGAAT
  CTCCACCAGAGGAAGGCTGGGAGGAGCAGATGTGTGTCAAACTCTCGGAGAACCGTGCCCGGAAAAAAAG
  GCTGAATATTATTATTGTGGCTGAAGGAGCAATTGATACCCAAAATAAACCCATCACCTCTGAGAAAATC
  AAAGAGCTTGTCGTCACGCAGCTGGGCTATGACACACGTGTGACCATCCTCGGGCACGTGCAGAGAGGAG
  GGACCCCTTCGGCATTCGACAGGATCTTGGCCAGCCGCATGGGAGTGGAGGCAGTCATCGCCTTGCTAGA
  GGCCACCCCGGACACCCCAGCTTGCGTCGTGTCACTGAACGGGAACCACGCCGTGCGCCTGCCGCTGATG
  GAGTGCGTGCAGATGACTCAGGATGTGCAGAAGGCGATGGACGAGAGGAGATTTCAAGATGCGGTTCGAC
  TCCGAGGGAGGAGCTTTGCGGGCAACCTGAACACCTACAAGCGACTTGCCATCAAGCTGCCGGATGATCA
  GATCCCAAAGACCAATTGCAACGTAGCTGTCATCAACGTGGGGGCACCCGCGGCTGGGATGAACGCAGCC
  GTACGCTCAGCTGTGCGCGTGGGCATTGCCGACGGCCACAGGATGCTCGCCATCTATGATGGCTTTGACG
  GCTTCGCCAAGGGCCAGATCAAAGAAATCGGCTGGACAGATGTCGGGGGCTGGACCGGCCAAGGAGGCTC
  CATTCTTGGGACAAAACGCGTTCTCCCGGGGAAGTACTTGGAAGAGATCGCCACACAGATGCGCACGCAC
  AGCATCAACGCGCTGCTGATCATCGGTGGATTCGAGGCCTACCTGGGACTCCTGGAGCTGTCAGCCGCCC
  GGGAGAAGCACGAGGAGTTCTGTGTCCCCATGGTCATGGTTCCCGCTACTGTGTCCAACAATGTGCCGGG
  TTCCGATTTCAGCATCGGGGCAGACACCGCCCTGAACACTATCACCGACACCTGCGACCGCATCAAGCAG
  TCCGCCAGCGGAACCAAGCGGCGCGTGTTCATCATCGAGACCATGGGCGGCTACTGTGGCTACCTGGCCA
  ACATGGGGGGGCTCGCGGCCGGAGCTGATGCCGCATACATTTTCGAAGAGCCCTTCGACATCAGGGATCT
  GCAGTCCAACGTGGAGCACCTGACGGAGAAAATGAAGACCACCATCCAGAGAGGCCTTGTGCTCAGAAAT
  GAGAGCTGCAGTGAAAACTACACCACCGACTTCATTTACCAGCTGTATTCAGAAGAGGGCAAAGGCGTGT
  TTGACTGCAGGAAGAACGTGCTGGGTCACATGCAGCAGGGTGGGGCACCCTCTCCATTTGATAGAAACTT
  TGGAACCAAAATCTCTGCCAGAGCTATGGAGTGGATCACTGCAAAACTCAAGGAGGCCCGGGGCAGAGGA
  AAAAAATTTACCACCGATGATTCCATTTGTGTGCTGGGAATAAGCAAAAGAAACGTTATTTTTCAACCTG
  TGGCAGAGCTGAAGAAGCAAACGGATTTTGAGCACAGGATTCCCAAAGAACAGTGGTGGCTCAAGCTACG
  GCCCCTCATGAAAATCCTGGCCAAGTACAAGGCCAGCTATGACGTGTCGGACTCAGGCCAGCTGGAACAT
  GTGCAGCCCTGGAGTGTCTGA
  
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001242339
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242339.1, NP_001229268.1
• RefSeq Size: 2781 bp
• RefSeq ORF: 2331 bp
• Locus ID: 5214
• UniProt ID: Q01813
• Cytogenetics: 10p15.2
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway
• Gene Summary: This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]
  Transcript Variant: This variant (2) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences causes translation initiation at an alternate AUG and results an isoform (2) that is shorter and has a distinct N-terminus, compared to isoform 1.