Nicastrin (NCSTN) (NM_001290184) Human Untagged Clone

CAT#: SC337208

NCSTN (untagged) - Human nicastrin (NCSTN), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCSTN
• Synonyms: ATAG1874
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001290184, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACTTTAATCTCATTTTAGAAAGTTTGTGCAGGGGAAACTCAGTGGAGAGGAAGATATATATCCCCT
  TAAATAAAACAGCTCCCTGTGTTCGCCTGCTCAACGCCACTCATCAGATTGGCTGCCAGTCTTCAATTAG
  TGGAGACACAGGGGTTATCCACGTAGTAGAGAAAGAGGAGGACCTACAGTGGGTATTGACTGATGGCCCC
  AACCCCCCTTACATGGTTCTGCTGGAGAGCAAGCATTTTACCAGGGATTTAATGGAGAAGCTGAAAGGGA
  GAACCAGCCGAATTGCTGGTCTTGCAGTGTCCTTGACCAAGCCCAGTCCTGCCTCAGGCTTCTCTCCTAG
  TGTACAGTGCCCAAATGATGGGTTTGGTGTTTACTCCAATTCCTATGGGCCAGAGTTTGCTCACTGCAGA
  GAAATACAGTGGAATTCGCTGGGCAATGGTTTGGCTTATGAAGACTTTAGTTTCCCCATCTTTCTTCTTG
  AAGATGAAAATGAAACCAAAGTCATCAAGCAGTGCTATCAAGATCACAACCTGAGTCAGAATGGCTCAGC
  ACCAACCTTCCCACTATGTGCCATGCAGCTCTTTTCACACATGCATGCTGTCATCAGCACTGCCACCTGC
  ATGCGGCGCAGCTCCATCCAAAGCACCTTCAGCATCAACCCAGAAATCGTCTGTGACCCCCTGTCTGATT
  ACAATGTGTGGAGCATGCTAAAGCCTATAAATACAACTGGGACATTAAAGCCTGACGACAGGGTTGTGGT
  TGCTGCCACCCGGCTGGATAGTCGTTCCTTTTTCTGGAATGTGGCCCCAGGGGCTGAAAGCGCAGTGGCT
  TCCTTTGTCACCCAGCTGGCTGCTGCTGAAGCTTTGCAAAAGGCACCTGATGTGACCACCCTGCCCCGCA
  ATGTCATGTTTGTCTTCTTTCAAGGGGAAACTTTTGACTACATTGGCAGCTCGAGGATGGTCTACGATAT
  GGAGAAGGGCAAGTTTCCCGTGCAGTTAGAGAATGTTGACTCATTTGTGGAGCTGGGACAGGTGGCCTTA
  AGAACTTCATTAGAGCTTTGGATGCACACAGATCCTGTTTCTCAGAAAAATGAGTCTGTACGGAACCAGG
  TGGAGGATCTCCTGGCCACATTGGAGAAGAGTGGTGCTGGTGTCCCTGCTGTCATCCTCAGGAGGCCAAA
  TCAGTCCCAGCCTCTCCCACCATCTTCCCTGCAGCGATTTCTTCGAGCTCGAAACATCTCTGGCGTTGTT
  CTGGCTGACCACTCTGGTGCCTTCCATAACAAATATTACCAGAGTATTTACGACACTGCTGAGAACATTA
  ATGTGAGCTATCCCGAATGGCTGAGCCCTGAAGAGGACCTGAACTTTGTAACAGACACTGCCAAGGCCCT
  GGCAGATGTGGCCACGGTGCTGGGACGTGCTCTGTATGAGCTTGCAGGAGGAACCAACTTCAGCGACACA
  GTTCAGGCTGATCCCCAAACGGTTACCCGCCTGCTCTATGGGTTCCTGATTAAAGCCAACAACTCATGGT
  TCCAGTCTATCCTCAGGCAGGACCTAAGGTCCTACTTGGGTGACGGGCCTCTTCAACATTACATCGCTGT
  CTCCAGCCCCACCAACACCACTTATGTTGTACAGTATGCCTTGGCAAATTTGACTGGCACAGTGGTCAAC
  CTCACCCGAGAGCAGTGCCAGGATCCAAGTAAAGTCCCAAGTGAAAACAAGGATCTGTATGAGTACTCAT
  GGGTCCAGGGCCCTTTGCATTCTAATGAGACGGACCGACTCCCCCGGTGTGTGCGTTCTACTGCACGATT
  AGCCAGGGCCTTGTCTCCTGCCTTTGAACTGAGTCAGTGGAGCTCTACTGAATACTCTACATGGACTGAG
  AGCCGCTGGAAAGATATCCGTGCCCGGATATTTCTCATCGCCAGCAAAGAGCTTGAGTTGATCACCCTGA
  CAGTGGGCTTCGGCATCCTCATCTTCTCCCTCATCGTCACCTACTGCATCAATGCCAAAGCTGATGTCCT
  TTTCATTGCTCCCCGGGAGCCAGGAGCTGTGTCATACTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290184
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290184.1, NP_001277113.1
• RefSeq Size: 3083 bp
• RefSeq ORF: 2070 bp
• Locus ID: 23385
• UniProt ID: Q92542
• Cytogenetics: 1q23.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Alzheimer's disease, Notch signaling pathway
• Gene Summary: This gene encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21. Alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (2) has a different 5' structure resulting in translation initiation at an alternate AUG compared to variant 1; the 5'-most AUG, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the alternate AUG resulting in a shorter isoform (2) with a distinct N-terminus compared to isoform 1.