VPS52 (NM_001289176) Human Untagged Clone

CAT#: SC336657

VPS52 (untagged) - Human vacuolar protein sorting 52 homolog (S. cerevisiae) (VPS52), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VPS52
• Synonyms: ARE1; dJ1033B10.5; SAC2; SACM2L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336657 representing NM_001289176.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGCAGATGTTGGGAGCTTTTCAGAGTGACCTCAGCTCCATCAGCTCTGAGATCCGGACACTGCAG
  GAACAGTCAGGAGCCATGAACATTCGACTTCGAAATCGCCAGGCAGTTCGGGGGAAACTTGGGGAGCTT
  GTTGATGGTCTGGTGGTGCCTTCTGCTCTGGTCACGGCAATTCTGGAGGCTCCAGTGACAGAGCCCAGG
  TTCTTGGAGCAGCTACAGGAGCTGGATGCCAAGGCAGCCGCAGTCAGAGAGCAGGAAGCTAGAGGCACA
  GCAGCCTGCGCAGATGTCAGAGGCGTGCTCGATCGGCTCCGGGTCAAGGCAGTGACGAAGATCCGAGAG
  TTTATCCTCCAGAAGATTTATTCCTTCAGGAAACCCATGACCAACTATCAGATCCCCCAGACGGCCCTG
  CTGAAGTACAGGTTCTTCTATCAGTTTCTGCTGGGCAATGAACGAGCAACAGCAAAGGAGATCAGGGAT
  GAATATGTGGAGACGCTGAGCAAGATTTACCTGTCTTACTACCGCTCTTACCTGGGGCGGCTCATGAAG
  GTGCAGTATCCATTTGAGGCCCTCTTCCGCAGCCAGCACTACGCCCTCCTAGACAATTCCTGCCGCGAA
  TACCTTTTCATCTGTGAATTTTTTGTTGTGTCTGGCCCAGCTGCACACGACCTGTTCCATGCTGTCATG
  GGCCGTACACTCAGCATGACCCTGAAACACCTGGATTCTTATCTAGCTGACTGCTACGATGCCATTGCT
  GTTTTTCTCTGTATCCACATTGTTCTCCGGTTCCGTAACATTGCAGCAAAGAGGGATGTTCCTGCCCTG
  GACAGGTACTGGGAACAGGTGCTTGCCTTGCTATGGCCACGGTTTGAACTGATCCTGGAGATGAATGTT
  CAGAGCGTCCGAAGCACTGACCCCCAGCGCCTAGGGGGGTTGGATACTCGGCCCCACTATATCACACGC
  CGCTATGCAGAGTTCTCCTCCGCTCTTGTCAGTATCAACCAGACAATTCCTAATGAACGGACCATGCAA
  TTGCTGGGACAGCTGCAGGTGGAGGTGGAGAATTTTGTCCTCCGAGTGGCAGCTGAGTTCTCCTCAAGG
  AAGGAGCAGCTTGTGTTTCTGATCAACAACTATGACATGATGCTGGGTGTGCTGATGGAGCGGGCTGCA
  GATGACAGCAAAGAGGTTGAGAGCTTCCAGCAGCTGCTCAATGCTCGGACACAGGAATTCATTGAAGAG
  TTGCTGTCTCCCCCTTTTGGGGGTTTAGTGGCATTTGTGAAGGAGGCTGAGGCTTTGATTGAGCGTGGA
  CAGGCTGAGCGACTTCGAGGGGAAGAAGCCCGGGTAACTCAGCTGATCCGTGGCTTTGGTAGTTCCTGG
  AAATCATCAGTGGAATCTCTGAGTCAGGATGTAATGCGGAGTTTCACCAACTTCAGAAATGGCACCAGT
  ATCATTCAGGGAGCGCTGACCCAGCTGATCCAGCTCTATCATCGCTTCCACCGGGTGCTGTCCCAGCCG
  CAGCTCCGAGCCCTCCCTGCCCGGGCTGAGCTCATCAACATTCACCACCTTATGGTGGAGCTCAAGAAG
  CATAAGCCCAACTTCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289176
• Insert Size: 1605 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289176.1
• RefSeq Size: 2535 bp
• RefSeq ORF: 1605 bp
• Locus ID: 6293
• UniProt ID: Q8N1B4
• Cytogenetics: 6p21.32
• MW: 61.4 kDa
• Gene Summary: This gene encodes a protein that is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and late endosomes, back to the trans-Golgi network. This gene is located on chromosome 6 in a head-to-head orientation with the gene encoding ribosomal protein S18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
  Transcript Variant: This variant (4) differs in the 5' UTR and the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 4, which is shorter than isoform 1.