GBA3 (NM_001277225) Human Untagged Clone

CAT#: SC336311

GBA3 (untagged) - Human glucosidase, beta, acid 3 (gene/pseudogene) (GBA3), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GBA3
• Synonyms: CBG; CBGL1; GLUC; KLRP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336311 representing NM_001277225.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCCCTTTACACTTGCTTGGTGGAGATAACTCTTCCCTTCCATTTAGAAGAAAACGTAGGAGGCTGG
  GATGCAGATGGAAAAGGCCCTTGTGTCTGGGACACATTTACTCATCAGGGAGGAGAGAGAGTTTTCAAG
  AACCAGACTGGCGATGTAGCTTGTGGCAGCTACACTCTGTGGGAGGAAGATTTGAAATGTATCAAACAG
  CTTGGATTGACTCATTACCGCTTCTCTCTTTCCTGGTCACGTCTGTTACCTGATGGGACGACAGGTTTC
  ATCAACCAGAAAGGAATTGATTATTACAACAAGATCATCGATGATTTGTTAAAAAATGGGGTTACTCCC
  ATTGTGACCCTCTACCACTTTGATTTGCCTCAGACTTTAGAAGACCAAGGAGGTTGGTTGTCAGAGGCA
  ATCATTGAATCCTTTGACAAATATGCTCAGTTTTGCTTCAGTACCTTTGGGGATCGTGTCAAGCAGTGG
  ATCACCATAAATGAAGCTAATGTTCTTTCTGTGATGTCATATGACTTAGGTATGTTTCCTCCGGGTATC
  CCTCACTTTGGGACTGGAGGTTATCAGGCAGCTCATAATTTGATTAAGGCTCATGCCAGATCCTGGCAC
  AGCTATGATTCCTTATTTCGAAAAAAGCAGAAAGGTATGGTGTCTCTATCACTTTTTGCGGTCTGGTTG
  GAACCAGCAGATCCCAACTCAGTGTCTGACCAGGAAGCTGCTAAAAGAGCCATCACTTTCCATCTGGAT
  TTATTTGCTAAACCCATATTCATCGATGGTGATTATCCTGAAGTTGTCAAGTCTCAGATTGCCTCCATG
  AGTCAAAAGCAAGGCTATCCATCATCGAGGCTTCCAGAATTCACTGAAGAAGAGAAGAAAATGATCAAA
  GGCACTGCTGATTTTTTTGCTGTGCAATATTATACAACTCGCTTAATCAAGTACCAGGAGAACAAGAAA
  GGAGAACTAGGTATTCTCCAGGATGCGGAAATTGAATTTTTTCCAGATCCATCTTGGAAAAATGTGGAT
  TGGATCTACGTGGTACCATGGGGAGTATGTAAACTACTGAAATATATTAAGGATACATATAATAACCCT
  GTAATTTACATCACTGAGAATGGGTTTCCCCAGAGTGACCCAGCGCCTCTTGATGACACTCAACGCTGG
  GAGTATTTCAGACAAACATTTCAGGAACTGTTCAAAGCTATCCAACTTGATAAAGTCAATCTTCAAGTA
  TATTGTGCATGGTCTCTTCTGGATAACTTTGAGTGGAACCAGGGATACAGCAGCCGGTTTGGTCTCTTC
  CACGTTGATTTTGAAGACCCAGCTAGACCCCGAGTCCCTTACACATCGGCCAAGGAATATGCCAAGATC
  ATCCGAAACAATGGCCTTGAAGCACATCTGTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001277225
• Insert Size: 1413 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001277225.1
• RefSeq Size: 2091 bp
• RefSeq ORF: 1413 bp
• Locus ID: 57733
• UniProt ID: Q9H227
• Cytogenetics: 4p15.2
• Protein Pathways: Cyanoamino acid metabolism, Starch and sucrose metabolism
• MW: 54.1 kDa
• Gene Summary: The protein encoded by this gene is an enzyme that can hydrolyze several types of glycosides. This gene is a polymorphic pseudogene, with the most common allele being the functional allele that encodes the full-length protein. Some individuals, as represented by the reference genome allele, contain a single nucleotide polymorphism that results in a premature stop codon in the coding region, and therefore this allele is pseudogenic due to the failure to produce a functional full-length protein. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Mar 2013]
  Transcript Variant: This variant (3, coding) contains an alternate 5' exon and it thus differs in the 5' UTR and 5' coding region, compared to variant 1. The encoded isoform (c) has a distinct N-terminus and is longer than isoform a. This variant is produced from the more frequently occurring functional allele of this gene.