PLAGL1 (NM_001289047) Human Untagged Clone

CAT#: SC336275

PLAGL1 (untagged) - Human pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 18

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLAGL1
• Synonyms: LOT1; ZAC; ZAC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336275 representing NM_001289047.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCACGTTCCCCTGCCAGTTATGTGGCAAGACGTTCCTCACCCTGGAGAAGTTCACGATTCACAAT
  TATTCCCACTCCAGGGAGCGGCCGTACAAGTGTGTGCAGCCTGACTGTGGCAAAGCCTTTGTTTCCAGA
  TATAAATTGATGAGGCATATGGCTACCCATTCTCCCCAGAAATCTCACCAGTGTGCTCACTGTGAGAAG
  ACGTTCAACCGGAAAGACCACCTGAAAAACCACCTCCAGACCCACGACCCCAACAAAATGGCCTTTGGG
  TGTGAGGAGTGTGGGAAGAAGTACAACACCATGCTGGGCTATAAGAGGCACCTGGCCCTCCATGCGGCC
  AGCAGTGGGGACCTCACCTGTGGGGTCTGTGCCCTGGAGCTAGGGAGCACCGAGGTGCTACTGGACCAC
  CTCAAAGCCCATGCGGAAGAGAAGCCCCCTAGCGGAACCAAGGAAAAGAAGCACCAGTGCGACCACTGT
  GAAAGATGCTTCTACACCCGGAAGGATGTGCGACGCCACCTGGTGGTCCACACAGGATGCAAGGACTTC
  CTGTGCCAGTTCTGTGCCCAGAGATTTGGGCGCAAGGATCACCTCACCCGGCATACCAAGAAGACCCAC
  TCACAGGAGCTGATGAAAGAGAGCTTGCAGACCGGAGACCTTCTGAGCACCTTCCACACCATCTCGCCT
  TCATTCCAACTGAAGGCTGCTGCCTTGCCTCCTTTCCCTTTAGGAGCTTCTGCCCAGAACGGGCTTGCA
  AGTAGCTTGCCAGCTGAGGTCCATAGCCTCACCCTCAGTCCCCCAGAACAAGCCGCCCAGCCTATGCAG
  CCGCTGCCAGAGTCCCTGGCCTCCCTCCACCCCTCGGTATCCCCTGGCTCTCCTCCGCCACCCCTTCCC
  AATCACAAGTACAACACCACTTCTACCTCATACTCCCCACTTGCAAGCCTGCCCCTCAAAGCAGATACT
  AAAGGTTTTTGCAATATCAGTTTGTTTGAGGACTTGCCTCTGCAAGAGCCTCAGTCACCTCAAAAGCTC
  AACCCAGGTTTTGATCTGGCTAAGGGAAATGCTGGTAAAGTAAACCTGCCCAAGGAGCTGCCTGCAGAT
  GCTGTGAACCTAACAATACCTGCCTCTCTGGACCTGTCCCCCCTGTTGGGCTTCTGGCAGCTGCCCCCT
  CCTGCTACCCAAAATACCTTTGGGAATAGCACTCTTGCCCTGGGGCCTGGGGAATCTTTGCCCCACAGG
  TTAAGCTGTCTGGGGCAGCAGCAGCAAGAACCCCCACTTGCCATGGGCACTGTGAGCCTGGGCCAGCTC
  CCCCTGCCCCCCATCCCTCATGTGTTCTCAGCTGGCACTGGCTCTGCCATCCTGCCTCATTTCCATCAT
  GCATTCAGATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289047
• Insert Size: 1392 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289047.1
• RefSeq Size: 3254 bp
• RefSeq ORF: 1392 bp
• Locus ID: 5325
• UniProt ID: Q9UM63
• Cytogenetics: 6q24.2
• Protein Families: Transcription Factors
• MW: 50.8 kDa
• Gene Summary: This gene encodes a C2H2 zinc finger protein that functions as a suppressor of cell growth. This gene is often deleted or methylated and silenced in cancer cells. In addition, overexpression of this gene during fetal development is thought to be the causal factor for transient neonatal diabetes mellitus (TNDM). Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding two different protein isoforms. The P1 downstream promoter of this gene is imprinted, with preferential expression from the paternal allele in many tissues. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (18, also known as P1A) initiates from the P1 promoter and differs in the 5' UTR compared to variant 2. Variants 2, 3, 4, 5, 6, 10, 12, 15, 16, 17, 18, 19, 20, 22, 23, 25, 27, and 28 all encode the same isoform (2).