LONP1 (NM_001276480) Human Untagged Clone

CAT#: SC333258

LONP1 (untagged) - Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LONP1
• Synonyms: CODASS; hLON; LON; LonHS; LONP; PIM1; PRSS15
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC333258 representing NM_001276480.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGCAGGACCTTGGGGACAAGCTGCGCATGATCGTCATGGGACACAGAAGAGTCCATATCAGCAGACAG
  CTGGAGGTGGAGCCCGAGGAGCCGGAGGCGGAGAACAAGCACAAGCCCCGCAGGAAGTCAAAGCGGGGC
  AAGAAGGAGGCGGAGGACGAGCTGAGCGCCAGGCACCCGGCGGAGCTGGCGATGGAGCCCACCCCTGAG
  CTCCCGGCTGAGGTGCTCATGGTGGAGGTAGAGAACGTTGTCCACGAGGACTTCCAGGTCACGGAGGAG
  GTGAAAGCCCTGACTGCAGAGATCGTGAAGACCATCCGGGACATCATTGCCTTGAACCCTCTCTACAGG
  GAGTCAGTGCTGCAGATGATGCAGGCTGGCCAGCGGGTGGTGGACAACCCCATCTACCTGAGCGACATG
  GGCGCCGCGCTCACCGGGGCCGAGTCCCATGAGCTGCAGGACGTCCTGGAAGAGACCAATATTCCTAAG
  CGGCTGTACAAGGCCCTCTCCCTGCTGAAGAAGGAATTTGAACTGAGCAAGCTGCAGCAGCGCCTGGGG
  CGGGAGGTGGAGGAGAAGATCAAGCAGACCCACCGTAAGTACCTGCTGCAGGAGCAGCTAAAGATCATC
  AAGAAGGAGCTGGGCCTGGAGAAGGACGACAAGGATGCCATCGAGGAGAAGTTCCGGGAGCGCCTGAAG
  GAGCTCGTGGTCCCCAAGCACGTCATGGATGTTGTGGACGAGGAGCTGAGCAAGCTGGGCCTGCTGGAC
  AACCACTCCTCGGAGTTCAATGTCACCCGCAACTACCTAGACTGGCTCACGTCCATCCCTTGGGGCAAG
  TACAGCAACGAGAACCTGGACCTGGCGCGGGCACAGGCAGTGCTGGAGGAAGACCACTACGGCATGGAG
  GACGTCAAGAAACGCATCCTGGAGTTCATTGCCGTTAGCCAGCTCCGCGGCTCCACCCAGGGCAAGATC
  CTCTGCTTCTATGGCCCCCCTGGCGTGGGTAAGACCAGCATTGCTCGCTCCATCGCCCGCGCCCTGAAC
  CGAGAGTACTTCCGCTTCAGCGTCGGGGGCATGACTGACGTGGCTGAGATCAAGGGCCACAGGCGGACC
  TACGTGGGCGCCATGCCCGGGAAGATCATCCAGTGTTTGAAGAAGACCAAGACGGAGAACCCCCTGATC
  CTCATCGACGAGGTGGACAAGATCGGCCGAGGCTACCAGGGGGACCCGTCGTCGGCACTGCTGGAGCTG
  CTGGACCCAGAGCAGAATGCCAACTTCCTGGACCACTACCTGGACGTGCCCGTGGACTTGTCCAAGGTG
  CTGTTCATCTGCACGGCCAACGTCACGGACACCATCCCCGAGCCGCTGCGAGACCGTATGGAGATGATC
  AACGTGTCGGGCTACGTGGCCCAGGAGAAGCTGGCCATTGCGGAGCGCTACCTGGTGCCCCAGGCTCGC
  GCCCTGTGTGGCTTGGATGAGAGCAAGGCCAAGCTGTCATCGGACGTGCTGACGCTGCTCATCAAGCAG
  TACTGCCGCGAGAGCGGTGTCCGCAACCTGCAGAAGCAAGTGGAGAAGGTGTTACGGAAATCGGCCTAC
  AAGATTGTCAGCGGCGAGGCCGAGTCCGTGGAGGTGACGCCCGAGAACCTGCAGGACTTCGTGGGGAAG
  CCCGTGTTCACCGTGGAGCGCATGTATGACGTGACACCGCCCGGCGTGGTCATGGGGCTGGCCTGGACC
  GCAATGGGAGGCTCCACGCTGTTTGTGGAGACATCCCTGAGACGGCCACAGGACAAGGATGCCAAGGGT
  GACAAGGATGGCAGCCTGGAGGTGACAGGCCAGCTGGGGGAGGTGATGAAGGAGAGCGCCCGCATAGCC
  TACACCTTCGCCAGAGCCTTCCTCATGCAGCACGCCCCCGCCAATGACTACCTGGTGACCTCACACATC
  CACCTGCATGTGCCCGAGGGCGCCACCCCCAAGGACGGCCCAAGCGCAGGCTGCACCATCGTCACGGCC
  CTGCTGTCCCTGGCCATGGGCAGGCCTGTCCGGCAGAATCTGGCCATGACTGGCGAAGTCTCCCTCACG
  GGCAAGATCCTGCCTGTTGGTGGCATCAAGGAGAAGACCATTGCGGCCAAGCGCGCAGGGGTGACGTGC
  ATCGTCCTGCCAGCCGAGAACAAGAAGGACTTCTACGACCTGGCAGCCTTCATCACCGAGGGCCTGGAG
  GTGCACTTCGTGGAACACTACCGGGAGATCTTCGACATCGCCTTCCCGGACGAGCAGGCAGAGGCGCTG
  GCCGTGGAACGGTGA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276480
• Insert Size: 2292 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276480.1
• RefSeq Size: 2900 bp
• RefSeq ORF: 2292 bp
• Locus ID: 9361
• UniProt ID: P36776
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome, Protease
• MW: 85.6 kDa
• Gene Summary: This gene encodes a mitochondrial matrix protein that belongs to the Lon family of ATP-dependent proteases. This protein mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides in the mitochondrial matrix. It may also have a chaperone function in the assembly of inner membrane protein complexes, and participate in the regulation of mitochondrial gene expression and maintenance of the integrity of the mitochondrial genome. Decreased expression of this gene has been noted in a patient with hereditary spastic paraplegia (PMID:18378094). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2013]
  Transcript Variant: This variant (3) contains an alternate 5' terminal exon and uses an in-frame downstream start codon compared to variant 1. The encoded isoform (3) has a shorter N-terminus compared to isoform 1.