ACTN3 (NM_001258371) Human Untagged Clone

CAT#: SC332676

ACTN3 (untagged) - Homo sapiens actinin, alpha 3 (ACTN3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACTN3
• Synonyms: ACTN3D
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC332676 representing NM_001258371.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGGGGATAGGGCTCTGTGTCCCACCAGCGAGGTGCTGGAAGAGGCTCACGAGGAGCTCCAGAGGGCAG
  CCTCTAGAGCGGCCCGCGGCCACTATTATTATCGGCCTTTCTATTGTGGAGAGGAGTAAACAGACTCAG
  AGAGGTCCCGTGGATTTCTCAAGGTCACACAGCGCCCCAGCAGCGGAGCAGGAATGCAAACCCAGGATC
  TCCGCCGCGAGATCCACCCCGGCGCCCCCGAGTCCCGCAGTTCCCGGCTTCAGGACAACCCCTTGCCAG
  ACCTTCACTGCCTGGTGCAACTCACACCTGCGCAAGGCAGGCACCCAGATCGAGAACATCGAGGAAGAT
  TTCCGCAATGGCCTCAAACTCATGCTGCTCCTGGAGGTCATTTCAGGTGAGAGGCTGCCTAGGCCAGAT
  AAAGGCAAGATGCGCTTCCACAAAATCGCCAACGTTAACAAGGCCCTGGACTTCATTGCCAGCAAGGGG
  GTTAAACTGGTGTCCATTGGTGCTGAAGAGATTGTTGACGGGAACCTGAAGATGACCCTGGGCATGATC
  TGGACCATCATCCTTCGCTTCGCCATCCAGGACATCTCTGTGGAAGAAACCTCAGCCAAGGAAGGCTTG
  CTTCTGTGGTGCCAGAGGAAGACAGCACCGTACCGCAACGTCAACGTGCAGAACTTCCACACCAGCTGG
  AAGGATGGCCTGGCCCTCTGTGCCCTCATCCACCGACACCGCCCTGACCTCATCGACTACGCCAAACTG
  CGAAAGGATGACCCCATCGGAAACCTGAACACTGCCTTTGAGGTGGCAGAGAAATACCTGGACATCCCC
  AAGATGTTGGATGCAGAAGACATTGTGAACACCCCAAAGCCGGATGAGAAGGCCATCATGACCTATGTG
  TCCTGCTTCTACCATGCCTTTGCCGGGGCTGAGCAGGCAGAGACAGCTGCCAACAGGATCTGCAAGGTG
  CTGGCAGTGAACCAGGAAAACGAGAAGCTGATGGAGGAGTATGAGAAGCTTGCCAGTGAGCTGCTGGAG
  TGGATCCGCCGCACTGTCCCATGGCTGGAGAACCGTGTGGGTGAGCCCAGCATGAGTGCCATGCAGCGC
  AAACTAGAGGACTTTCGGGACTACCGGCGTCTGCACAAGCCGCCCCGCATTCAGGAAAAGTGCCAGCTG
  GAGATCAACTTCAACACACTGCAGACCAAGTTGCGGCTCAGCCACCGGCCTGCCTTCATGCCCTCCGAG
  GGCAAGCTGGTCTCGGACATCGCCAACGCCTGGCGGGGGCTGGAGCAGGTGGAAAAGGGCTATGAGGAC
  TGGCTGCTCTCGGAGATCCGGCGCCTGCAGCGACTCCAGCACCTGGCTGAGAAGTTCCGGCAGAAGGCC
  TCCCTGCACGAAGCCTGGACCCGGGGAAAGGAGGAGATGCTGAGCCAGCGCGACTACGATTCGGCTTTG
  CTACAGGAGGTGCGGGCGTTGCTGCGGCGCCACGAGGCCTTTGAGAGCGACCTGGCGGCGCACCAGGAC
  CGCGTGGAGCACATTGCCGCGCTGGCCCAGGAGCTCAATGAGCTGGACTACCACGAGGCAGCCTCAGTG
  AATAGCCGCTGCCAGGCCATCTGCGATCAGTGGGACAACCTGGGCACCCTGACCCAGAAGAGGCGGGAT
  GCGCTAGAGCGGATGGAGAAGCTCCTGGAGACCATTGACCAGCTGCAACTGGAGTTTGCCCGGCGGGCC
  GCGCCCTTCAACAACTGGCTGGATGGTGCCGTGGAGGACCTGCAGGACGTGTGGCTGGTACACTCTGTG
  GAGGAGACCCAGAGCCTGCTGACAGCGCACGATCAGTTCAAGGCAACACTGCCCGAGGCTGACCGAGAG
  CGAGGTGCCATCATGGGCATCCAGGGTGAGATCCAGAAGATCTGCCAGACGTATGGGCTGCGGCCCTGC
  TCCACCAATCCCTACATCACCCTCAGCCCGCAGGACATCAACACCAAGTGGGATATGGTCCGAAAGCTG
  GTGCCCAGCCGTGACCAGACACTGCAGGAGGAGCTGGCACGGCAGCAGGTAAACGAGAGGCTCCGGCGA
  CAGTTTGCGGCCCAGGCCAATGCCATTGGACCCTGGATCCAGGCGAAGGTGGAGGAAGTGGGGCGGCTG
  GCAGCAGGGCTAGCTGGCTCTCTGGAGGAGCAGATGGCTGGGCTACGGCAGCAGGAGCAGAACATTATC
  AACTACAAGACTAACATTGACCGGCTGGAGGGTGACCACCAGCTGCTGCAGGAGAGCCTGGTGTTCGAC
  AATAAGCACACCGTCTACAGCATGGAGCACATCCGCGTGGGCTGGGAGCAGCTGCTCACCTCCATTGCC
  CGCACCATCAATGAAGTGGAGAACCAGGTACTGACCCGAGACGCCAAGGGACTGAGCCAGGAGCAGCTC
  AACGAGTTCCGAGCATCCTTCAACCACTTTGACAGGAAGCGGAATGGGATGATGGAGCCTGATGACTTC
  CGAGCTTGCCTCATCTCCATGGGCTATGACCTGGGGGAAGTGGAGTTTGCTCGCATCATGACCATGGTG
  GACCCCAACGCAGCTGGGGTGGTGACCTTCCAGGCCTTCATAGACTTCATGACCCGAGAGACAGCCGAG
  ACTGACACGACTGAGCAAGTTGTAGCTTCCTTCAAGATCTTGGCAGGAGACAAGAACTACATCACCCCC
  GAGGAGCTGCGGCGCGAGCTCCCTGCCAAGCAGGCCGAGTACTGCATCCGCCGTATGGTGCCCTACAAG
  GGATCCGGGGCCCCGGCTGGAGCCCTGGACTACGTGGCCTTCTCCAGTGCCCTCTATGGGGAGAGCGAC
  CTTTGA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001258371
• Insert Size: 2835 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001258371.1
• RefSeq Size: 3086 bp
• RefSeq ORF: 2835 bp
• Locus ID: 89
• UniProt ID: Q08043
• Cytogenetics: 11q13.2
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction
• MW: 107.7 kDa
• Gene Summary: This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (2) uses an alternate exon in the 5' UTR and coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) is longer and has a distinct N-terminus, compared to isoform 1.