CLCN4 (NM_001256944) Human Untagged Clone

CAT#: SC332498

CLCN4 (untagged) - Homo sapiens chloride channel, voltage-sensitive 4 (CLCN4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLCN4
• Synonyms: ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC332498 representing NM_001256944.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGACGGACCTGAAGGAGGGGGTCTGCCTGTCTGCCTTCTGGTATAGCCATGAGCAGTGTTGCTGGACT
  TCTAACGAGACCACTTTTGAGGACAGAGACAAGTGTCCCCTGTGGCAGAAATGGTCGGAGCTGCTGGTG
  AATCAGTCAGAGGGTGCCAGTGCTTACATTCTGAATTACTTAATGTACATCCTATGGGCGCTGCTGTTT
  GCATTTTTGGCTGTCTCCCTGGTGCGTGTATTTGCACCATATGCCTGTGGCTCTGGCATACCAGAGATA
  AAGACCATTTTGAGCGGCTTTATCATCAGGGGCTACTTGGGGAAGTGGACCCTGCTAATCAAGACAGTC
  ACGCTGGTGCTGGTAGTGTCCTCCGGTCTGAGCCTTGGGAAGGAAGGGCCGCTAGTGCACGTGGCTTGT
  TGCTGTGGCAACTTCTTCAGCAGCCTTTTCTCCAAGTACAGCAAGAATGAGGGCAAGAGGCGGGAGGTG
  CTTTCAGCTGCAGCGGCTGCTGGAGTCTCTGTTGCCTTTGGTGCACCAATTGGAGGCGTGCTTTTCAGT
  CTAGAAGAGGTCAGTTACTACTTTCCCCTGAAGACCTTGTGGAGGTCATTTTTCGCAGCCCTGGTGGCG
  GCCTTTACGCTGAGATCCATCAATCCCTTTGGGAATAGCCGTCTCGTTCTCTTTTATGTGGAATACCAC
  ACGCCCTGGTACATGGCTGAACTCTTCCCCTTCATCCTGCTTGGGGTCTTCGGGGGCTTGTGGGGAACC
  CTCTTCATCCGCTGCAACATCGCCTGGTGCAGGAGGCGCAAGACCACCAGGCTGGGGAAGTACCCGGTG
  CTGGAGGTCATTGTGGTGACTGCCATCACTGCCATCATTGCCTACCCCAATCCCTACACACGCCAGAGC
  ACCAGCGAGCTCATTTCTGAGCTGTTCAATGACTGTGGAGCCCTTGAGTCTTCCCAGCTCTGTGACTAC
  ATCAATGACCCCAACATGACTCGGCCTGTGGATGACATTCCAGACCGGCCGGCTGGTGTCGGTGTTTAC
  ACGGCCATGTGGCAGCTGGCCCTGGCACTGATCTTCAAAATCGTCGTTACCATATTTACCTTTGGCATG
  AAGATCCCGTCGGGCCTCTTCATCCCCAGCATGGCTGTGGGCGCGATAGCGGGCAGGATGGTGGGAATT
  GGCGTGGAGCAGCTGGCCTACCATCACCATGACTGGATCATCTTCAGGAACTGGTGCAGACCCGGTGCA
  GACTGTGTCACGCCAGGGCTGTACGCAATGGTGGGAGCTGCGGCCTGCCTCGGTGGAGTTACCAGGATG
  ACGGTGTCATTGGTGGTCATCATGTTTGAATTAACCGGGGGTCTGGAGTACATCGTGCCCCTGATGGCG
  GCGGCTGTGACCAGCAAGTGGGTAGCTGATGCATTTGGGAAAGAAGGCATCTACGAGGCCCACATCCAC
  TTAAATGGGTACCCTTTCCTTGACGTGAAGGACGAGTTTACTCACCGCACACTGGCCACCGACGTCATG
  CGGCCCCGGCGGGGAGAGCCGCCACTGTCGGTGCTCACCCAGGACAGCATGACTGTCGAGGACGTGGAG
  ACGCTCATCAAGGAGACCGACTACAACGGCTTCCCCGTGGTGGTCTCCAGAGACTCCGAGCGCCTCATT
  GGATTTGCCCAGAGGAGGGAACTGATTCTCGCAATAAAGAACGCCAGACAGAGGCAGGAGGGCATTGTG
  AGCAATTCCATCATGTACTTCACGGAGGAACCCCCCGAGCTGCCGGCCAACAGCCCACATCCCCTGAAG
  CTGCGGCGCATCCTGAACCTCAGCCCGTTTACAGTGACAGACCACACTCCGATGGAAACGGTGGTGGAT
  ATCTTCCGGAAACTGGGGCTTCGGCAGTGCCTGGTGACGCGGAGCGGGAGACTTCTTGGCATCATCACA
  AAAAAGGATGTTCTGAGACATATGGCCCAGATGGCAAACCAGGACCCCGAATCCATCATGTTTAATTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001256944
• Insert Size: 2001 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256944.1
• RefSeq Size: 6534 bp
• RefSeq ORF: 2001 bp
• Locus ID: 1183
• UniProt ID: P51793
• Cytogenetics: Xp22.2
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• MW: 74.3 kDa
• Gene Summary: The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. Chloride channel 4 has an evolutionary conserved CpG island and is conserved in both mouse and hamster. This gene is mapped in close proximity to APXL (Apical protein Xenopus laevis-like) and OA1 (Ocular albinism type I), which are both located on the human X chromosome at band p22.3. The physiological role of chloride channel 4 remains unknown but may contribute to the pathogenesis of neuronal disorders. Alternate splicing results in two transcript variants that encode different proteins. [provided by RefSeq, Mar 2012]
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.