USP15 (NM_001252078) Human Untagged Clone

CAT#: SC332171

USP15 (untagged) - Homo sapiens ubiquitin specific peptidase 15 (USP15), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: USP15
• Synonyms: UNPH-2; UNPH4
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC332171 representing NM_001252078.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGGCGGAAGGCGGAGCGGCGGATCTGGACACCCAGCGGTCTGACATCGCGACGCTGCTCAAAACCTCG
  CTCCGGAAAGGGGACACCTGGTACCTAGTCGATAGTCGCTGGTTCAAACAGTGGAAAAAATATGTTGGC
  TTTGACAGTTGGGACAAATACCAGATGGGAGATCAAAATGTGTATCCTGGACCCATTGATAACTCTGGA
  CTTCTCAAAGATGGTGATGCCCAGTCACTTAAGGAACACCTTATTGATGAATTGGATTACATACTGTTG
  CCAACTGAAGGTTGGAATAAACTTGTCAGCTGGTACACATTGATGGAAGGTCAAGAGCCAATAGCACGA
  AAGGTGGTTGAACAGGGTATGTTTGTAAAGCACTGCAAAGTAGAAGTATATCTCACAGAATTGAAGCTA
  TGTGAAAATGGAAACATGAATAATGTTGTAACTCGAAGATTTAGCAAAGCTGACACAATAGATACAATT
  GAAAAGGAAATAAGAAAAATCTTCAGTATTCCAGATGAAAAGGAGACCAGATTGTGGAACAAATACATG
  AGTAACACATTTGAACCACTGAATAAACCAGACAGCACCATTCAGGATGCTGGTTTATACCAAGGACAG
  GTATTAGTGATAGAACAGAAAAATGAAGATGGAACATGGCCAAGGGGTCCTTCTACTCCTAAGTCCCCA
  GGTGCATCCAATTTTTCAACTTTACCAAAGATCTCTCCTTCATCTCTATCAAATAATTATAACAACATG
  AACAACAGAAATGTGAAAAACTCAAATTACTGTCTTCCATCATATACCGCTTATAAGAACTATGATTAT
  TCGGAACCTGGAAGAAACAATGAACAGCCAGGCCTCTGTGGCCTAAGTAACTTGGGAAATACGTGTTTC
  ATGAACTCAGCTATTCAGTGTTTGAGCAACACACCTCCACTTACTGAGTATTTCCTCAATGATAAGTAT
  CAAGAAGAACTGAATTTTGACAATCCCTTAGGAATGAGAGGTGAAATAGCTAAATCTTATGCCGAACTG
  ATCAAGCAAATGTGGTCTGGAAAGTTTAGCTACGTCACCCCAAGAGCCTTTAAGACACAGGTAGGACGT
  TTTGCACCTCAGTTCTCTGGATATCAGCAGCAAGACTGTCAAGAACTGTTAGCTTTCCTATTAGATGGA
  TTACATGAGGATTTGAATAGAATTAGGAAAAAACCATATATACAATTAAAAGATGCAGATGGAAGGCCA
  GATAAGGTGGTTGCCGAAGAAGCCTGGGAAAACCATTTAAAACGAAATGATTCTATCATAGTAGATATA
  TTTCATGGCCTTTTCAAATCAACTTTAGTTTGTCCTGAGTGTGCTAAGATTTCAGTAACATTTGATCCT
  TTTTGTTACTTGACACTTCCATTGCCCATGAAAAAAGAACGCACCTTGGAAGTTTACTTAGTTAGAATG
  GATCCACTTACCAAACCTATGCAGTACAAAGTGGTTGTCCCCAAAATTGGAAACATATTAGATCTTTGT
  ACAGCATTGTCTGCTTTGTCAGGAATACCTGCAGATAAGATGATAGTTACTGATATATACAATCATAGA
  TTTCACAGAATATTCGCTATGGATGAAAACCTTAGTAGTATTATGGAACGGGATGATATTTATGTGTTT
  GAAATTAACATCAATAGGACAGAAGATACAGAGCACGTGATTATTCCTGTTTGCCTAAGAGAAAAATTC
  AGACACTCGAGTTATACCCACCATACTGGTTCTTCACTTTTTGGTCAGCCCTTTCTTATGGCTGTACCA
  CGAAACAATACTGAAGACAAACTTTATAATCTCCTGCTCTTGAGAATGTGCCGATATGTCAAAATATCT
  ACTGAAACTGAAGAAACTGAAGGATCCCTACACTGCTGTAAGGACCAAAATATTAATGGGAATGGCCCA
  AATGGCATACATGAAGAAGGCTCACCAAGTGAAATGGAAACAGATGAGCCAGATGATGAATCCAGCCAG
  GATCAAGAACTTCCCTCAGAGAATGAAAACAGTCAGTCTGAAGATTCAGTTGGAGGAGATAATGATTCT
  GAAAATGGATTATGTACTGAGGATACTTGCAAAGGTCAACTCACGGGACACAAAAAACGATTGTTTACA
  TTCCAGTTCAACAACTTAGGCAATACTGATATCAACTACATCAAAGATGATACCAGGCATATAAGATTT
  GATGATAGGCAGCTTAGGCTAGATGAAAGATCTTTTCTTGCTTTGGATTGGGATCCTGATTTGAAAAAA
  AGATATTTTGATGAAAATGCTGCTGAGGACTTTGAAAAACATGAAAGTGTGGAGTATAAACCTCCTAAA
  AAACCCTTTGTGAAATTAAAAGATTGCATTGAACTTTTTACAACAAAAGAAAAGCTAGGTGCTGAAGAT
  CCCTGGTATTGTCCGAATTGTAAAGAACATCAGCAAGCCACAAAGAAATTGGATTTATGGTCCCTGCCT
  CCAGTACTTGTAGTACATCTCAAGCGATTTTCTTACAGTCGATACATGAGAGACAAGTTGGATACCTTA
  GTTGATTTTCCTATCAATGACTTGGATATGTCGGAATTCTTAATTAATCCAAATGCAGGTCCTTGCCGC
  TATAATCTGATTGCTGTTTCCAACCACTATGGAGGGATGGGAGGAGGACACTATACTGCTTTTGCAAAA
  AATAAAGATGATGGAAAATGGTACTATTTTGATGACAGTAGTGTCTCCACTGCATCTGAAGACCAAATT
  GTGTCCAAAGCAGCATATGTACTCTTCTACCAGAGACAAGACACTTTCAGTGGAACTGGCTTTTTTCCT
  CTTGACCGAGAAACTAAAGGTGCTTCAGCTGCCACTGGCATCCCATTAGAAAGTGATGAAGATAGCAAT
  GATAATGACAATGATATAGAAAATGAAAACTGTATGCACACTAACTAA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252078
• Insert Size: 2946 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252078.1
• RefSeq Size: 8367 bp
• RefSeq ORF: 2946 bp
• Locus ID: 9958
• UniProt ID: Q9Y4E8
• Cytogenetics: 12q14.1
• Protein Families: Druggable Genome, Protease
• MW: 112.4 kDa
• Gene Summary: This gene encodes a member of the ubiquitin specific protease (USP) family of deubiquitinating enzymes. USP enzymes play critical roles in ubiquitin-dependent processes through polyubiquitin chain disassembly and hydrolysis of ubiquitin-substrate bonds. The encoded protein associates with the COP9 signalosome, and also plays a role in transforming growth factor beta signalling through deubiquitination of receptor-activated SMAD transcription factors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 2. [provided by RefSeq, Nov 2011]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.