ACSF3 (NM_001243279) Human Untagged Clone

CAT#: SC331987

ACSF3 (untagged) - Homo sapiens acyl-CoA synthetase family member 3 (ACSF3), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACSF3
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC331987 representing NM_001243279.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGCTGCCCCATGTGGTGCTCACCTTCCGGCGCCTGGGCTGCGCCTTGGCGTCCTGCCGGCTGGCGCCT
  GCGAGACACAGAGGAAGTGGTCTTCTGCACACAGCCCCAGTGGCCCGCTCGGACAGGAGCGCCCCGGTG
  TTCACCCGTGCCCTGGCCTTTGGGGACAGAATCGCCCTGGTTGACCAGCACGGCCGCCACACGTACAGG
  GAGCTTTATTCCCGCAGCCTTCGCCTGTCCCAGGAGATCTGCAGGCTCTGCGGGTGTGTCGGCGGGGAC
  CTCCGGGAGGAGAGGGTCTCCTTCCTATGCGCTAACGATGCCTCCTACGTCGTGGCCCAGTGGGCGTCA
  TGGATGAGTGGCGGTGTGGCAGTCCCCCTCTACAGGAAGCATCCCGCGGCCCAGCTGGAGTATGTCATC
  TGCGACTCCCAGAGCTCTGTGGTCCTTGCCAGCCAGGAGTACCTGGAGCTCCTGAGCCCGGTGGTCAGG
  AAGCTGGGGGTCCCGCTGCTGCCGCTCACACCAGCCATCTACACTGGAGCAGTAGAGGAACCGGCAGAG
  GTCCCGGTCCCAGAGCAGGGATGGAGGAACAAGGGCGCCATGATCATCTACACCAGTGGGACCACGGGG
  AGGCCCAAGGGCGTGCTGAGCACGCACCAAAACATCAGGGCTGTGGTGACCGGGCTGGTCCACAAGTGG
  GCATGGACCAAAGACGACGTGATCCTCCACGTGCTCCCGCTGCACCACGTCCATGGTGTGGTCAACGCG
  CTGCTCTGTCCTCTCTGGGTGGGAGCCACCTGTGTGATGATGCCTGAGTTCAGCCCTCAGCAGGTTTGG
  GAAAAGTTCTTAAGTTCTGAAACGCCGCGGATCAATGTCTTTATGGCAGTGCCTACAATATACACCAAG
  CTGATGGAGTACTACGACAGGCATTTTACCCAGCCGCACGCCCAGGATTTCTTGCGTGCAGTTTGTGAA
  GAAAAAATTAGGCTGATGGTCTCAGGCTCAGCTGCCCTGCCCCTCCCAGTGCTGGAGAAGTGGAAGAAC
  ATCACGGGCCACACCCTGCTGGAGCGGTATGGCATGACCGAGATCGGCATGGCTCTGTCCGGGCCCCTG
  ACCACTGCCGTGCGCCTGCCAGGTTCCGTGGGGACCCCACTGCCTGGAGTACAGGTGCGCATTGTCTCA
  GAAAACCCACAGAGGGAAGCCTGCTCCTACACCATCCACGCAGAGGGAGACGAGAGGGGGACCAAGGTG
  ACCCCAGGGTTTGAAGAAAAGGAGGGGGAGCTGCTGGTGAGGGGACCCTCCGTGTTTCGAGAATACTGG
  AATAAACCAGAAGAAACTAAGAGTGCATTCACCCTGGATGGCTGGTTTAAGACAGGGGACACCGTGGTG
  TTTAAGGATGGCCAGTACTGGATCCGAGGCCGGACCTCAGTGGACATCATCAAGACTGGAGGCTACAAG
  GTCAGCGCCCTGGAGGTGGAGTGGCACCTGCTGGCCCACCCCAGCATCACAGATGTGGCTGTGATTGGA
  GTTCCGGATATGACATGGGGCCAGCGGGTCACTGCTGTGGTGACCCTCCGAGAAGGACACTCACTGTCC
  CACAGGGAGCTCAAAGAGTGGGCCAGAAATGTCCTGGCCCCGTACGCGGTGCCCTCGGAGCTGGTGCTG
  GTGGAGGAGATCCCGCGGAACCAGATGGGCAAGATTGACAAGAAGGCGCTCATCAGGCACTTCCACCCC
  TCATGA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001243279
• Insert Size: 1731 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243279.2
• RefSeq Size: 3751 bp
• RefSeq ORF: 1731 bp
• Locus ID: 197322
• UniProt ID: Q4G176
• Cytogenetics: 16q24.3
• MW: 64.1 kDa
• Gene Summary: This gene encodes a member of the acyl-CoA synthetase family of enzymes that activate fatty acids by catalyzing the formation of a thioester linkage between fatty acids and coenzyme A. The encoded protein is localized to mitochondria, has high specificity for malonate and methylmalonate and possesses malonyl-CoA synthetase activity. Mutations in this gene are a cause of combined malonic and methylmalonic aciduria. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Sep 2013]
  Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.