Phospholipase D2 (PLD2) (NM_001243108) Human Untagged Clone

CAT#: SC331947

PLD2 (untagged) - Homo sapiens phospholipase D2 (PLD2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Phospholipase D2
• Synonyms: PLD1C
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC331947 representing NM_001243108.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGACGGCGACCCCTGAGAGCCTCTTCCCCACTGGGGACGAACTGGACTCCAGCCAGCTCCAGATGGAG
  TCCGATGAGGTGGACACCCTGAAGGAGGGAGAGGACCCAGCCGACCGGATGCACCCGTTTCTGGCCATC
  TATGAGCTTCAGTCTCTGAAAGTGCACCCCTTGGTGTTCGCACCTGGGGTCCCTGTCACAGCCCAGGTG
  GTGGGCACCGAAAGATATACCAGCGGATCCAAGGTGGGAACCTGCACTCTGTATTCTGTCCGCTTGACT
  CACGGCGACTTTTCCTGGACAACCAAGAAGAAATACCGTCATTTTCAGGAGCTGCATCGGGACCTCCTG
  AGACACAAAGTCTTGATGAGTCTGCTCCCTCTGGCTCGATTTGCCGTTGCCTATTCTCCAGCCCGAGAT
  GCAGGCAACAGAGAGATGCCCTCTCTACCCCGGGCAGGTCCTGAGGGCTCCACCAGACATGCAGCCAGC
  AAACAGAAATACCTGGAGAATTACCTCAACCGTCTCTTGACCATGTCTTTCTATCGCAACTACCATGCC
  ATGACAGAGTTCCTGGAAGTCAGTCAGCTGTCCTTTATCCCGGACTTGGGCCGCAAAGGACTGGAGGGG
  ATGATCCGGAAGCGCTCAGGTGGCCACCGTGTTCCTGGCCTCACCTGCTGTGGCCGAGACCAAGTTTGT
  TATCGCTGGTCCAAGAGGTGGCTGGTGGTGAAGGACTCCTTCCTGCTGTACATGTGCCTCGAGACAGGT
  GCCATCTCATTTGTTCAGCTCTTTGACCCTGGCTTTGAGGTGCAAGTGGGGAAAAGGAGCACGGAGGCA
  CGGCACGGCGTGCGGATCGATACCTCCCACAGGTCCTTGATTCTCAAGTGCAGCAGCTACCGGCAGGCA
  CGGTGGTGGGCCCAAGAGATCACTGAGCTGGCACAGGGCCCAGGCAGAGACTTCCTACAGCTGCACCGG
  CATGACAGCTACGCCCCACCCCGGCCTGGGACCTTGGCCCGGTGGTTTGTGAATGGGGCAGGTTACTTT
  GCTGCTGTGGCAGATGCCATCCTTCGAGCTCAAGAGGAGATTTTCATCACAGACTGGTGGTTGAGTCCT
  GAGGTTTACCTGAAGCGTCCGGCCCATTCAGATGACTGGAGACTGGACATTATGCTCAAGAGGAAGGCG
  GAGGAGGGTGTCCGTGTGTCTATTCTGCTGTTTAAAGAAGTGGAATTGGCCTTGGGCATCAACAGTGGC
  TATAGCAAGAGGGCGCTGATGCTGCTGCACCCCAACATAAAGGTGATGCGTCACCCAGACCAAGTGACG
  TTGTGGGCCCATCATGAGAAGCTCCTGGTGGTGGACCAAGTGGTAGCATTCCTGGGGGGACTGGACCTT
  GCCTATGGCCGCTGGGATGACCTGCACTACCGACTGACTGACCTTGGAGACTCCTCTGAATCAGCTGCC
  TCCCAGCCTCCCACCCCGCGCCCAGACTCACCAGCCACCCCAGACCTCTCTCACAACCAATTCTTCTGG
  CTGGGCAAGGACTACAGCAATCTTATCACCAAGGACTGGGTGCAGCTGGACCGGCCTTTCGAAGATTTC
  ATTGACAGGGAGACGACCCCTCGGATGCCATGGCGGGACGTTGGGGTGGTCGTCCATGGCCTACCGGCC
  CGGGACCTTGCCCGGCACTTCATCCAGCGCTGGAACTTCACCAAGACCACCAAGGCCAAGTACAAGACT
  CCCACATACCCCTACCTGCTTCCCAAGTCTACCAGCACGGCCAATCAGCTCCCCTTCACACTTCCAGGA
  GGGCAGTGCACCACCGTACAGGTCTTGCGATCAGTGGACCGCTGGTCAGCAGGGACTCTGGAGAACTCC
  ATCCTCAATGCCTACCTGCACACCATCAGGGAGAGCCAGCACTTCCTCTACATTGAGAATCAGTTCTTC
  ATTAGCTGCTCAGATGGGCGGACGGTTCTGAACAAGGTGGGCGATGAGATTGTGGACAGAATCCTGAAG
  GCCCACAAACAGGGGTGGTGTTACCGAGTCTACGTGCTTTTGCCCTTACTCCCTGGCTTCGAGGGTGAC
  ATCTCCACGGGCGGTGGCAACTCCATCCAGGCCATTCTGCACTTTACTTACAGGACCCTGTGTCGTGGG
  GAGTATTCAATCCTGCATCGCCTTAAAGCAGCCATGGGGACAGCATGGCGGGACTATATTTCCATCTGC
  GGGCTTCGTACACACGGAGAGCTGGGCGGGCACCCCGTCTCGGAGCTCATCTACATCCACAGCAAGGTG
  CTCATCGCAGATGACCGGACAGTCATCATTGGTTCTGCAAACATCAATGACCGGAGCTTGCTGGGGAAG
  CGGGACAGTGAGCTGGCCGTGCTGATCGAGGACACAGAGACGGAACCATCCCTCATGAATGGGGCAGAG
  TATCAGGCGGGCAGTGTGATTCTTGGAGCAAATACCCGGCCAGACTTGGATCTCCGAGACCCCATCTGT
  GATGACTTCTTCCAGTTGTGGCAAGACATGGCTGAGAGCAACGCCAATATCTATGAGCAGATCTTCCGC
  TGCCTGCCATCCAATGCCACGCGTTCCCTGCGGACTCTCCGGGAGTACGTGGCCGTGGAGCCCTTGGCC
  ACGGTCAGTCCCCCCTTGGCTCGGTCTGAGCTCACCCAGGTCCAGGGCCACCTGGTCCACTTCCCCCTC
  AAGTTCCTAGAGGATGAGTCTTTGCTGCCCCCGCTGGGTAGCAAGGAGGGCATGATCCCCCTAGAAGTG
  TGGACATAG
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001243108
• Insert Size: 2769 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243108.1
• RefSeq Size: 3481 bp
• RefSeq ORF: 2769 bp
• Locus ID: 5338
• UniProt ID: O14939
• Cytogenetics: 17p13.2
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Ether lipid metabolism, Fc gamma R-mediated phagocytosis, Glycerophospholipid metabolism, GnRH signaling pathway, Metabolic pathways
• MW: 104.7 kDa
• Gene Summary: The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (PLD2B) has the same N- and C-termini but is shorter compared to isoform PLD2A.