KIFAP3 (NM_001204516) Human Untagged Clone

CAT#: SC331599

KIFAP3 (untagged) - Homo sapiens kinesin-associated protein 3 (KIFAP3), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KIFAP3
• Synonyms: dJ190I16.1; FLA3; KAP-1; KAP-3; KAP3; SMAP; Smg-GDS
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC331599 representing NM_001204516.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGCAAGGGGAGGACGCCAGATACCTCAAAAGCATTCGACTTAAGAGTCTCAATGCCAACACAGATATA
  ACTTCCCTGGCAAGGAAGGTGGTTGAAGAATGTAAACTCATTCATCCTTCAAAACTAAATGAGGTAGAA
  CAGCTGTTGTACTATCTACAGAACCGCCGTGATTCATTGTCAGGAAAAGAGAAAAAAGAAAAATCAAGC
  AAGCCTAAAGATCCACCTCCTTTTGAAGGAATGGAGATTGATGAAGTTGCTAACATTAATGACATGGAT
  GAATATATTGAGTTATTATATGAAGATATTCCTGACAAAGTTCGGGGTTCTGCTTTGATCCTGCAGCTT
  GCTCGAAATCCTGATAACTTGGAAGAACTACTATTGAATGAAACTGCCCTTGGTGCATTAGCAAGGGTC
  CTGAGAGAAGACTGGAAGCAAAGTGTCGAGTTAGCTACAAACATAATTTACATCTTTTTTTGTTTCTCC
  AGCTTTTCTCAATTTCATGGACTTATTACTCACTATAAAATTGGAGCTCTGTGTATGAATATTATTGAT
  CATGAGTTAAAAAGACATGAGCTTTGGCAAGAAGAACTCTCAAAGAAGAAGAAAGCTGTTGATGAAGAC
  CCTGAAAACCAAACCTTGAGAAAGGATTATGAAAAAACCTTTAAAAAGTACCAGGGGCTTGTGGTAAAA
  CAGGAACAGCTATTACGAGTTGCTCTTTATTTGCTTCTGAATCTTGCTGAGGATACTCGTACCGAACTG
  AAAATGAGGAACAAGAACATAGTTCACATGTTGGTGAAAGCCCTTGATCGGGACAATTTTGAGCTGCTA
  ATTTTAGTTGTGTCATTCTTGAAGAAACTCAGCATTTTTATGGAGAATAAAAATGATATGGTGGAAATG
  GATATTGTTGAAAAACTGGTGAAAATGATACCTTGTGAGCATGAAGACCTGCTGAATATCACCCTCCGA
  CTTTTACTAAACCTATCCTTTGACACAGGACTGAGGAATAAGATGGTACAAGTTGGACTGCTTCCCAAG
  CTCACTGCACTCCTAGGCAATGACAACTACAAACAAATAGCAATGTGTGTTCTTTACCACATAAGCATG
  GATGACCGCTTTAAATCAATGTTTGCATACACTGACTGTATACCACAGTTAATGAAGATGCTGTTTGAA
  TGTTCAGATGAACGAATTGACTTGGAACTCATTTCTTTCTGCATTAATCTTGCTGCTAACAAAAGAAAT
  GTACAGCTTATCTGTGAAGGAAATGGGCTGAAGATGCTCATGAAGAGGGCTCTGAAGTTTAAGGATCCA
  TTGCTGATGAAAATGATTAGAAACATTTCTCAGCATGATGGACCAACTAAAAATCTGTTTATTGATTAT
  GTTGGGGACCTTGCAGCCCAGATCTCTAATGATGAAGAAGAGGAGTTTGTGATTGAATGTTTGGGAACT
  CTTGCAAACTTGACCATTCCAGACTTAGACTGGGAATTGGTTCTTAAAGAATATAAGTTGGTTCCATAC
  CTCAAGGATAAACTAAAACCAGGTGCTGCAGAAGATGATCTTGTTTTAGAAGTGGTTATAATGATTGGA
  ACTGTATCCATGGATGACTCTTGTGCTGCATTGCTAGCCAAATCTGGCATAATCCCTGCACTCATTGAA
  TTGCTAAATGCTCAACAAGAAGATGATGAATTTGTGTGTCAGATAATTTATGTCTTCTACCAGATGGTT
  TTCCACCAAGCCACAAGAGACGTCATAATCAAGGAAACACAGGCTCCAGCATATCTCATAGACCTAATG
  CATGATAAGAATAATGAAATCCGAAAGGTCTGTGATAATACATTAGATATTATAGCGGAATATGATGAA
  GAATGGGCTAAGAAAATTCAGAGTGAAAAGTTTCGCTGGCATAACTCTCAGTGGCTGGAGATGGTAGAG
  AGTCGTCAGATGGATGAGAGTGAGCAGTACTTGTATGGTGATGATCGAATTGAGCCATACATTCATGAA
  GGAGATATTCTCGAAAGACCTGACCTTTTCTACAACTCAGATGGATTAATTGCCTCTGAAGGAGCCATA
  AGTCCCGATTTCTTCAATGATTACCACCTTCAAAATGGAGATGTTGTTGGGCAGCATTCATTTCCTGGC
  AGCCTTGGAATGGATGGCTTTGGCCAACCAGTTGGCATTCTTGGACGCCCTGCCACAGCATATGGATTC
  CGCCCTGATGAACCTTACTACTATGGCTATGGATCTTGA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001204516
• Insert Size: 2247 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001204516.1
• RefSeq Size: 2879 bp
• RefSeq ORF: 2247 bp
• Locus ID: 22920
• UniProt ID: Q92845
• Cytogenetics: 1q24.2
• Protein Families: Druggable Genome
• MW: 86.3 kDa
• Gene Summary: The small G protein GDP dissociation stimulator (smg GDS) is a regulator protein having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions, and the other is to inhibit their interactions with membranes. The protein encoded by this gene contains 9 'Armadillo' repeats and interacts with the smg GDS protein through these repeats. This protein, which is highly concentrated around the endoplasmic reticulum, is phosphorylated by v-src, and this phosphorylation reduces the affinity of the protein for smg GDS. It is thought that this protein serves as a linker between human chromosome-associated polypeptide (HCAP) and KIF3A/B, a kinesin superfamily protein in the nucleus, and that it plays a role in the interaction of chromosomes with an ATPase motor protein. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1.