KLHL3 (NM_001257194) Human Untagged Clone

CAT#: SC330575

KLHL3 (untagged) - Homo sapiens kelch-like family member 3 (KLHL3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KLHL3
• Synonyms: PHA2D
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC330575 representing NM_001257194.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGGGGAAAGCATTCAAGGTTATGAATGAACTGCGGAGTAAACAGCTGTTGTGTGACGTGATGATTGTG
  GCAGAAGATGTCGAGATAGAAGCCCACCGTGTGGTCCTGGCAGCCTGCAGCCCCTACTTCTGTGCGATG
  TTCACAGGTGACATGTCTGAGAGTAAAGCCAAAAAGATAGAAATCAAGGACGTGGATGGGCAGACGCTG
  AGTAAGCTGATTGACTACATCTATACTGCTGAAATCGAGGTGACTGAAGAGAATGTCCAGGTGCTGCTC
  CCGGCAGCCAGCTTGCTGCAGCTCATGGATGTTCGGCAGAACTGCTGTGACTTCCTGCAGTCTCAGTTG
  CATCCCACCAATTGCCTGGGCATCCGTGCATTTGCAGATGTACACACCTGCACTGACCTTCTGCAGCAG
  GCCAATGCCTACGCAGAGCAGCACTTTCCAGAGGTGATGCTAGGAGAAGAATTTCTTAGCCTGAGTCTG
  GACCAGGTGTGCAGCTTGATATCCAGCGACAAGCTGACCGTTTCTTCAGAAGAGAAGGTGTTTGAAGCT
  GTGATCTCATGGATCAATTATGAGAAAGAAACCCGTTTAGAGCACATGGCAAAGCTGATGGAACATGTC
  CGACTTCCTCTCTTACCTAGGGACTACCTAGTCCAAACGGTTGAAGAAGAAGCTTTGATAAAGAATAAC
  AACACCTGTAAAGACTTCCTCATTGAGGCCATGAAATACCATCTCCTCCCTCTGGATCAGAGACTATTG
  ATTAAGAACCCAAGGACCAAGCCCAGGACTCCAGTCAGCCTTCCCAAGGTCATGATTGTGGTTGGCGGC
  CAGGCACCCAAGGCAATCCGCAGTGTGGAGTGCTATGATTTCGAGGAGGACCGGTGGGATCAGATTGCT
  GAGCTTCCTTCCAGAAGATGCAGAGCAGGTGTGGTGTTCATGGCTGGCCACGTGTATGCCGTGGGAGGG
  TTTAATGGCTCACTGCGGGTGCGGACAGTGGATGTGTATGACGGCGTGAAGGACCAGTGGACGTCCATT
  GCCAGCATGCAGGAGCGCCGGAGCACACTGGGCGCAGCGGTGCTCAATGACTTGCTCTACGCAGTGGGA
  GGCTTTGATGGCAGTACTGGCCTAGCATCGGTGGAAGCCTACAGCTACAAGACCAACGAGTGGTTCTTT
  GTGGCCCCGATGAACACGCGGCGGAGCAGTGTGGGTGTGGGCGTTGTGGAGGGGAAGCTATATGCTGTT
  GGGGGTTATGATGGAGCTTCCCGCCAGTGTCTGAGCACTGTGGAGCAGTACAACCCAGCGACCAATGAA
  TGGATATACGTGGCGGACATGAGCACCCGCCGCAGTGGCGCAGGGGTTGGAGTGCTTAGCGGACAGCTG
  TACGCCACAGGTGGGCATGATGGGCCTTTGGTGAGGAAGAGCGTTGAGGTTTACGATCCTGGAACAAAT
  ACCTGGAAGCAAGTGGCAGACATGAACATGTGCCGGCGCAACGCAGGGGTCTGTGCAGTAAATGGGCTC
  CTGTATGTGGTTGGAGGGGATGATGGATCCTGCAACTTGGCTTCGGTGGAGTACTACAATCCTGTCACT
  GACAAATGGACGCTGCTTCCAACGAACATGAGCACGGGGCGGAGCTATGCAGGTGTTGCCGTGATTCAC
  AAGTCCTTGTGA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001257194
• Insert Size: 1668 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001257194.1
• RefSeq Size: 6981 bp
• RefSeq ORF: 1668 bp
• Locus ID: 26249
• UniProt ID: Q9UH77
• Cytogenetics: 5q31.2
• MW: 61.5 kDa
• Gene Summary: This gene is ubiquitously expressed and encodes a full-length protein which has an N-terminal BTB domain followed by a BACK domain and six kelch-like repeats in the C-terminus. These kelch-like repeats promote substrate ubiquitination of bound proteins via interaction of the BTB domain with the CUL3 (cullin 3) component of a cullin-RING E3 ubiquitin ligase (CRL) complex. Muatations in this gene cause pseudohypoaldosteronism type IID (PHA2D); a rare Mendelian syndrome featuring hypertension, hyperkalaemia and metabolic acidosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]
  Transcript Variant: This variant (2) lacks a 5' coding exon and has an alternate 5' exon which results in the use of a downstream in-frame start codon, compared to variant 1. These differences result in a protein (isoform 2; also known as KLHL3b) with a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.