NDUFS1 (NM_001199982) Human Untagged Clone

CAT#: SC329606

NDUFS1 (untagged) - Homo sapiens NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase) (NDUFS1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDUFS1
• Synonyms: CI-75k; CI-75Kd; MC1DN5; PRO1304
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC329606 representing NM_001199982.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGTGGGAAGGTGTGATGGAGTTCTTATTAGCAAATCACCCATTGGACTGTCCTATTTGTGACCAGGGA
  GGTGAATGTGATCTGCAGGACCAGTCCATGATGTTTGGAAATGATAGGAGCCGATTTTTAGAGGGGAAG
  CGTGCTGTGGAAGACAAGAACATTGGGCCATTGGTAAAGACCATCATGACAAGATGTATACAGTGTACT
  CGCTGCATCAGGTTTGCAAGTGAGATTGCAGGAGTAGATGATTTGGGAACAACAGGCAGAGGAAATGAT
  ATGCAAGTTGGCACATACATTGAAAAGATGTTCATGTCTGAACTGTCTGGGAATATCATTGATATCTGC
  CCTGTAGGTGCCCTAACCTCTAAGCCCTATGCCTTTACTGCCCGGCCTTGGGAAACAAGAAAGACAGAA
  TCCATTGATGTAATGGATGCGGTTGGAAGTAATATTGTGGTTAGCACAAGAACTGGAGAAGTGATGAGG
  ATTTTGCCACGTATGCATGAGGACATCAATGAAGAGTGGATCTCTGATAAAACCAGATTTGCCTATGAT
  GGGCTAAAACGTCAAAGACTTACCGAGCCAATGGTCAGAAATGAAAAAGGGCTTTTAACCTATACTTCT
  TGGGAGGATGCGCTCTCTCGCGTAGCTGGAATGTTGCAGAGTTTTCAAGGCAAAGATGTGGCAGCAATT
  GCAGGTGGCTTGGTGGATGCTGAAGCCCTGGTAGCTCTCAAAGATTTGCTTAATAGAGTGGACTCTGAC
  ACCTTATGCACTGAAGAGGTCTTCCCCACTGCAGGAGCTGGCACAGATTTGCGTTCCAATTATCTTCTT
  AATACTACAATTGCTGGTGTGGAAGAGGCAGATGTTGTTCTTCTGGTTGGTACAAACCCACGTTTTGAG
  GCACCACTGTTTAATGCTAGAATTCGAAAGAGCTGGCTGCATAATGACTTAAAAGTGGCCCTTATAGGC
  AGTCCAGTGGACCTCACTTACACATATGACCACCTGGGAGACTCCCCCAAAATTCTTCAAGACATTGCT
  TCGGGAAGCCATCCATTTAGCCAGGTCCTAAAGGAAGCTAAAAAACCAATGGTGGTTTTAGGCAGTTCT
  GCACTCCAAAGAAATGATGGAGCAGCAATTCTTGCAGCTGTTTCTAGCATTGCACAAAAGATTCGGATG
  ACTAGTGGTGTTACTGGTGATTGGAAAGTTATGAATATCCTTCATAGGATTGCAAGTCAAGTAGCTGCT
  TTGGACCTTGGCTATAAGCCTGGGGTGGAAGCAATTCGGAAGAACCCTCCCAAGGTGCTGTTTCTCCTG
  GGAGCAGATGGAGGTTGTATCACACGACAGGATTTGCCAAAGGATTGTTTCATTATTTATCAAGGACAT
  CATGGTGATGTTGGGGCTCCCATAGCTGATGTTATTCTCCCAGGAGCTGCTTACACAGAGAAGTCTGCT
  ACATATGTCAACACTGAGGGTAGAGCTCAGCAGACTAAGGTAGCAGTGACACCTCCTGGCTTGGCAAGA
  GAAGACTGGAAAATTATAAGAGCACTCTCTGAGATTGCTGGAATGACTCTTCCATATGATACTCTGGAT
  CAAGTAAGGAACAGATTGGAAGAAGTCTCTCCTAATCTTGTTCGATATGATGATATTGAAGGGGCTAAT
  TACTTCCAGCAAGCAAATGAGCTCTCAAAGCTAGTGAACCAGCAGCTTCTTGCTGACCCACTTGTTCCA
  CCTCAGCTAACTATAAAAGACTTCTACATGACAGATTCAATTAGCAGAGCCTCACAGACAATGGCCAAA
  TGTGTCAAAGCTGTCACAGAGGGTGCCCAGGCAGTAGAGGAACCATCCATATGCTGA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001199982
• Insert Size: 1851 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199982.1
• RefSeq Size: 3196 bp
• RefSeq ORF: 1851 bp
• Locus ID: 4719
• UniProt ID: P28331
• Cytogenetics: 2q33.3
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• MW: 67.5 kDa
• Gene Summary: The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
  Transcript Variant: This variant (3) lacks an alternate coding segment compared to variant 1, that causes a frameshift. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1.