SEMA6D (NM_001198999) Human Untagged Clone

CAT#: SC329482

SEMA6D (untagged) - Homo sapiens sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant 7

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SEMA6D
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC329482 representing NM_001198999.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGAGGGTCTTCCTGCTTTGTGCCTACATACTGCTGCTGATGGTTTCCCAGTTGAGGGCAGTCAGCTTT
  CCTGAAGATGATGAACCCCTTAATACTGTCGACTATCACTATTCAAGGCAATATCCGGTTTTTAGAGGA
  CGCCCTTCAGGCAATGAATCGCAGCACAGGCTGGACTTTCAGCTGATGTTGAAAATTCGAGACACACTT
  TATATTGCTGGCAGGGATCAAGTTTATACAGTAAACTTAAATGAAATGCCCAAAACAGAAGTAATACCC
  AACAAGAAACTGACATGGCGATCAAGACAACAGGATCGAGAAAACTGTGCTATGAAAGGCAAGCATAAA
  GATGAATGCCACAACTTTATCAAAGTATTTGTTCCAAGAAACGATGAGATGGTTTTTGTTTGTGGTACC
  AATGCATTCAATCCCATGTGTAGATACTACAGGTTGAGTACCTTAGAATATGATGGGGAAGAAATTAGT
  GGCCTGGCAAGATGCCCATTTGATGCCAGACAAACCAATGTTGCCCTCTTTGCTGATGGGAAGCTGTAT
  TCTGCCACAGTGGCTGACTTCTTGGCCAGCGATGCCGTTATTTATCGAAGCATGGGTGATGGATCTGCC
  CTTCGCACAATAAAATATGATTCCAAATGGATAAAAGAGCCACACTTTCTTCATGCCATAGAATATGGA
  AACTATGTCTATTTCTTCTTTCGAGAAATCGCTGTCGAACATAATAATTTAGGCAAGGCTGTGTATTCC
  CGCGTGGCCCGCATATGTAAAAACGACATGGGTGGTTCCCAGCGGGTCCTGGAGAAACACTGGACTTCA
  TTTCTAAAGGCTCGGCTGAACTGTTCTGTCCCTGGAGATTCGTTTTTCTACTTTGATGTTCTGCAGTCT
  ATTACAGACATAATACAAATCAATGGCATCCCCACTGTGGTCGGGGTGTTTACCACGCAGCTCAATAGC
  ATCCCTGGTTCTGCTGTCTGTGCATTTAGCATGGATGACATTGAAAAAGTATTCAAAGGACGGTTTAAG
  GAACAGAAAACTCCAGATTCTGTTTGGACAGCAGTTCCCGAAGACAAAGTGCCAAAGCCAAGGCCTGGC
  TGTTGTGCAAAACACGGCCTTGCCGAAGCTTATAAAACCTCCATCGATTTCCCGGATGAAACTCTGTCA
  TTCATCAAATCTCATCCCCTGATGGACTCTGCCGTTCCACCCATTGCCGATGAGCCCTGGTTCACAAAG
  ACTCGGGTCAGGTACAGACTGACGGCCATCTCAGTGGACCATTCAGCCGGACCCTACCAGAACTACACA
  GTCATCTTTGTTGGCTCTGAAGCTGGCATGGTACTTAAAGTTCTGGCAAAGACCAGTCCTTTCTCTTTG
  AACGACAGCGTATTACTGGAAGAGATTGAAGCCTACAACCATGCAAAGTGCAGTGCTGAGAATGAGGAA
  GACAAAAAGGTCATCTCATTACAGTTGGATAAAGATCACCACGCTTTATATGTGGCGTTCTCTAGCTGC
  ATTATCCGCATCCCCCTCAGTCGCTGTGAGCGTTATGGATCATGTAAAAAGTCTTGTATTGCATCTCGT
  GACCCGTATTGTGGCTGGTTAAGCCAGGGATCCTGTGGTAGAGTGACCCCAGGGATGCTGCTGTTAACC
  GAAGACTTCTTTGCTTTCCATAACCACAGTGCTGAAGGATATGAACAAGACACAGAATTCGGCAACACA
  GCTCATCTAGGGGACTGCCATGGTGTACGATGGGAAGTCCAGTCTGGAGAGTCCAACCAGATGGTCCAC
  ATGAATGTCCTCATCACCTGTGTCTTTGCTGCTTTTGTTTTGGGGGCATTCATTGCAGGTGTGGCAGTA
  TACTGCTATCGAGACATGTTTGTTCGGAAAAACAGAAAGATCCATAAAGATGCAGAGTCCGCCCAGTCA
  TGCACAGACTCCAGTGGAAGTTTTGCCAAACTGAATGGTCTCTTTGACAGCCCTGTCAAGGAATACCAA
  CAGAATATTGATTCTCCTAAACTGTATAGTAACCTGCTAACCAGTCGGAAAGAGCTACCACCCAATGGA
  GATACTAAATCCATGGTAATGGACCATCGAGGGCAACCTCCAGAGTTGGCTGCTCTTCCTACTCCTGAG
  TCTACACCCGTGCTTCACCAGAAGACCCTGCAGGCCATGAAGAGCCACTCAGAAAAGGCCCATGGCCAT
  GGAGCTTCAAGGAAAGAAACCCCTCAGTTTTTTCCGTCTAGTCCGCCACCTCATTCCCCATTAAGTCAT
  GGGCATATCCCCAGTGCCATTGTTCTTCCAAATGCTACCCATGACTACAACACGTCTTTCTCAAACTCC
  AATGCTCACAAAGCTGAAAAGAAGCTTCAAAACATTGATCACCCTCTCACAAAGTCATCCAGTAAGAGA
  GATCACCGGCGTTCTGTTGATTCCAGAAATACCCTCAATGATCTCCTGAAGCATCTGAATGACCCAAAT
  AGTAACCCCAAAGCCATCATGGGAGACATCCAGATGGCACACCAGAACTTAATGCTGGATCCCATGGGA
  TCGATGTCTGAGGTCCCACCTAAAGTCCCTAACCGGGAGGCATCGCTATACTCCCCTCCTTCAACTCTC
  CCCAGAAATAGCCCAACCAAGCGAGTGGATGTCCCCACCACTCCTGGAGTCCCAATGACTTCTCTGGAA
  AGACAAAGAGGTTATCACAAAAATTCCTCCCAGAGGCACTCTATATCTGCTATGCCTAAAAACTTAAAC
  TCACCAAATGGTGTTTTGTTATCCAGACAGCCTAGTATGAACCGTGGAGGATATATGCCCACCCCCACT
  GGGGCGAAGGTGGACTATATTCAGGGAACACCAGTGAGTGTTCATCTGCAGCCTTCCCTCTCCAGACAG
  AGCAGCTACACCAGTAATGGCACTCTTCCTAGGACGGGACTAAAGAGGACGCCGTCCTTAAAACCTGAC
  GTGCCACCAAAGCCTTCCTTTGTTCCTCAAACCCCATCTGTCAGACCACTGAACAAATACACATACTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001198999
• Insert Size: 3036 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198999.1
• RefSeq Size: 5935 bp
• RefSeq ORF: 3036 bp
• Locus ID: 80031
• UniProt ID: Q8NFY4
• Cytogenetics: 15q21.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Axon guidance
• MW: 113.3 kDa
• Gene Summary: Semaphorins are a large family, including both secreted and membrane associated proteins, many of which have been implicated as inhibitors or chemorepellents in axon pathfinding, fasciculation and branching, and target selection. All semaphorins possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Additional sequence motifs C-terminal to the semaphorin domain allow classification into distinct subfamilies. Results demonstrate that transmembrane semaphorins, like the secreted ones, can act as repulsive axon guidance cues. This gene encodes a class 6 vertebrate transmembrane semaphorin that demonstrates alternative splicing. Several transcript variants have been identified and expression of the distinct encoded isoforms is thought to be regulated in a tissue- and development-dependent manner. [provided by RefSeq, Nov 2010]
  Transcript Variant: This variant (7) uses an alternative splice site on one exon, lacks two internal exons, and differs in the 5' UTR, compared to variant 4. The encoded isoform (1) is missing an internal region compared to isoform 4 and contains an internal segment unique t