AREB6 (ZEB1) (NM_001174095) Human Untagged Clone

CAT#: SC329269

ZEB1 (untagged)-Human zinc finger E-box binding homeobox 1 (ZEB1) transcript variant 8

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ZEB1
• Synonyms: AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3; TCF8; ZFHEP; ZFHX1A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001174095, the custom clone sequence may differ by one or more nucleotides
  ATGGCGGATGGCCCCAGGTGTAAGCGCAGAAAGCAGGCGAACCCGCGGCGCAATAACGGA
  AAGGAAGGGCAAGAAATCCTGGGGCCTGAAGCTCAGGCAGATGAAGCAGGATGTACAGTA
  AAAGATGATGAATGCGAGTCAGATGCAGAAAATGAGCAAAACCATGATCCTAATGTTGAA
  GAGTTTCTACAACAACAAGACACTGCTGTCATTTTTCCTGAGGCACCTGAAGAGGACCAG
  AGGCAGGGCACACCAGAAGCCAGTGGTCATGATGAAAATGGAACACCAGATGCATTTTCA
  CAATTACTCACCTGTCCATATTGTGATAGAGGCTATAAACGCTTTACCTCTCTGAAAGAA
  CACATTAAATATCGTCATGAAAAGAATGAAGATAACTTTAGTTGCTCCCTGTGCAGTTAC
  ACCTTTGCATACAGAACCCAACTTGAACGTCACATGACATCACATAAATCAGGAAGAGAT
  CAAAGACATGTGACGCAGTCTGGGTGTAATCGTAAATTCAAATGCACTGAGTGTGGAAAA
  GCTTTCAAATACAAACATCACCTAAAAGAGCACTTAAGAATTCACAGTGGAGAGAAGCCA
  TATGAATGCCCAAACTGCAAGAAACGCTTTTCCCATTCTGGCTCCTATAGCTCACACATA
  AGCAGTAAGAAATGTATCAGCTTGATACCTGTGAATGGGCGACCAAGAACAGGACTCAAG
  ACATCTCAGTGTTCTTCACCGTCTCTTTCAGCATCACCAGGCAGTCCCACACGACCACAG
  ATACGGCAAAAGATAGAGAATAAACCCCTTCAAGAACAACTTTCTGTTAACCAAATTAAA
  ACTGAACCTGTGGATTATGAATTCAAACCCATAGTGGTTGCTTCAGGAATCAACTGTTCA
  ACCCCTTTACAAAATGGGGTTTTCACTGGTGGTGGCCCATTACAGGCAACCAGTTCTCCT
  CAGGGCATGGTGCAAGCTGTTGTTCTGCCAACAGTTGGTTTGGTGTCTCCCATAAGTATC
  AATTTAAGTGATATTCAGAATGTACTTAAAGTGGCGGTAGATGGTAATGTAATAAGGCAA
  GTGTTGGAGAATAATCAAGCCAATCTTGCATCCAAAGAACAAGAAACAATCAATGCTTCA
  CCCATACAACAAGGTGGCCATTCTGTTATTTCAGCCATCAGTCTTCCTTTGGTTGATCAA
  GATGGAACAACCAAAATTATCATCAACTACAGTCTTGAGCAGCCTAGCCAACTTCAAGTT
  GTTCCTCAAAATTTAAAAAAAGAAAATCCAGTCGCTACAAACAGTTGTAAAAGTGAAAAG
  TTACCAGAAGATCTTACTGTTAAGTCTGAGAAGGACAAAAGCTTTGAAGGGGGGGTGAAT
  GATAGCACTTGTCTTCTGTGTGATGATTGTCCAGGAGATATTAATGCACTTCCAGAATTA
  AAGCACTATGACCTAAAGCAGCCTACTCAGCCTCCTCCACTCCCTGCAGCAGAAGCTGAG
  AAGCCTGAGTCCTCTGTTTCATCAGCTACTGGAGATGGCAATTTGTCTCCTAGTCAGCCA
  CCTTTAAAGAACCTCTTGTCTCTCCTAAAAGCATATTATGCTTTGAATGCACAACCAAGT
  GCAGAAGAGCTCTCAAAAATTGCTGATTCAGTAAACCTACCACTGGATGTAGTAAAAAAG
  TGGTTTGAAAAGATGCAAGCTGGACAGATTTCAGTGCAGTCTTCTGAACCATCTTCTCCT
  GAACCAGGCAAAGTAAATATCCCTGCCAAGAACAATGATCAGCCTCAATCTGCAAATGCA
  AATGAACCCCAGGACAGCACAGTAAATCTACAAAGTCCTTTGAAGATGACTAACTCCCCA
  GTTTTACCAGTGGGATCAACCACCAATGGTTCCAGAAGTAGTACACCATCCCCATCACCT
  CTAAACCTTTCCTCATCCAGAAATACACAGGGTTACTTGTACACAGCTGAGGGTGCACAA
  GAAGAGCCACAAGTAGAACCTCTTGATCTTTCACTACCAAAGCAACAGGGAGAATTATTA
  GAAAGGTCAACTATCACTAGTGTTTACCAGAACAGTGTTTATTCTGTCCAGGAAGAACCC
  TTGAACTTGTCTTGCGCAAAAAAGGAGCCACAAAAGGACAGTTGTGTTACAGACTCAGAA
  CCAGTTGTAAATGTAATCCCACCAAGTGCCAACCCCATAAATATCGCTATACCTACAGTC
  ACTGCCCAGTTACCCACAATCGTGGCCATTGCTGACCAGAACAGTGTTCCATGCTTAAGA
  GCGCTAGCTGCCAATAAGCAAACGATTCTGATTCCCCAGGTGGCATACACCTACTCAACT
  ACGGTCAGCCCTGCAGTCCAAGAACCACCCTTGAAAGTGATCCAGCCAAATGGAAATCAG
  GATGAAAGACAAGATACTAGCTCAGAAGGAGTATCAAATGTAGAGGATCAGAATGACTCT
  GATTCTACACCGCCCAAAAAGAAAATGCGGAAGACAGAAAATGGAATGTATGCTTGTGAT
  TTGTGTGACAAGATATTCCAAAAGAGTAGTTCATTATTGAGACATAAATATGAACACACA
  GGTAAAAGACCTCATGAGTGTGGAATCTGTAAAAAGGCATTTAAACACAAACATCATTTG
  ATTGAACACATGCGATTACATTCTGGAGAAAAGCCCTATCAATGTGACAAATGTGGAAAG
  CGCTTCTCACACTCTGGGTCTTATTCTCAACACATGAATCATCGCTACTCCTACTGTAAG
  AGAGAAGCGGAAGAACGTGACAGCACAGAGCAGGAAGAGGCAGGGCCTGAAATCCTCTCG
  AATGAGCACGTGGGTGCCAGGGCGTCTCCCTCACAGGGCGACTCGGACGAGAGAGAGAGT
  TTGACAAGGGAAGAGGATGAAGACAGTGAAAAAGAGGAAGAGGAGGAGGATAAAGAGATG
  GAAGAATTGCAGGAAGAAAAAGAATGTGAAAAACCACAAGGGGATGAGGAAGAGGAGGAG
  GAGGAGGAAGAAGTGGAAGAAGAAGAGGTAGAAGAGGCAGAGAATGAGGGAGAAGAAGCA
  AAAACTGAAGGTCTGATGAAGGATGACAGGGCTGAAAGTCAAGCAAGCAGCTTAGGACAA
  AAAGTAGGCGAGAGTAGTGAGCAAGTGTCTGAAGAAAAGACAAATGAAGCCTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001174095
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001174095.1, NP_001167566.1
• RefSeq Size: 5797 bp
• RefSeq ORF: 3174 bp
• Locus ID: 6935
• UniProt ID: P37275
• Cytogenetics: 10p11.22
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010]
  Transcript Variant: This variant (8) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. This variant also lacks an in-frame exon and uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The encoded isoform (e) is shorter and has a distinct N-terminus, compared to isoform a.