STIM2 (NM_001169118) Human Untagged Clone

CAT#: SC329117

STIM2 (untagged)-Human stromal interaction molecule 2 (STIM2) transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STIM2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC329117 sequence for NM_001169118 edited (data generated by NextGen Sequencing)
  
  
  TTGCTGGTGCTCGGGCTGCTGGTAGCCGGAGCGGCGGACGGATGCGAGCTTGTGCCCCGGCACCTCCGCG
  GGCGGCGGGCGACTGGCTCTGCCGCAACTGCCGCCTCCTCTCCCGCCGCGGCGGCCGGCGATAGCCCGGC
  GCTCATGACAGATCCCTGCATGTCACTGAGTCCACCATGCTTTACAGAAGAAGACAGATTTAGTCTGGAA
  GCTCTTCAAACAATACATAAACAAATGGATGATGACAAAGATGGTGGAATTGAAGTAGAGGAAAGTGATG
  AATTCATCAGAGAAGATATGAAATATAAAGATGCTACTAATAAACACAGCCATCTGCACAGAGAAGATAA
  ACATATAACGATTGAGGATTTATGGAAACGATGGAAAACATCAGAAGTTCATAATTGGACCCTTGAAGAC
  ACTCTTCAGTGGTTGATAGAGTTTGTTGAACTACCCCAATATGAGAAGAATTTTAGAGACAACAATGTCA
  AAGGAACGACACTTCCCAGGATAGCAGTGCACGAACCTTCATTTATGATCTCCCAGTTGAAAATCAGTGA
  CCGGAGTCACAGACAAAAACTTCAGCTCAAGGCATTGGATGTGGTTTTGTTTGGACCTCTAACACGCCCA
  CCTCATAACTGGATGAAAGATTTTATCCTCACAGTTTCTATAGTAATTGGTGTTGGAGGCTGCTGGTTTG
  CTTATACGCAGAATAAGACATCAAAAGAACATGTTGCAAAAATGATGAAAGATTTAGAGAGCTTACAAAC
  TGCAGAGCAAAGTCTAATGGACTTACAAGAGAGGCTTGAAAAGGCACAGGAAGAAAACAGAAATGTTGCT
  GTAGAAAAGCAAAATTTAGAGCGCAAAATGATGGATGAAATCAATTATGCAAAGGAGGAGGCTTGTCGGC
  TGAGAGAGCTAAGGGAGGGAGCTGAATGTGAATTGAGTAGACGTCAGTATGCAGAACAGGAATTGGAACA
  GGTTCGCATGGCTCTGAAAAAGGCCGAAAAAGAATTTGAACTGAGAAGCAGTTGGTCTGTTCCAGATGCA
  CTTCAGAAATGGCTTCAGTTAACACATGAAGTAGAAGTGCAATACTACAATATTAAAAGACAAAACGCTG
  AAATGCAGCTAGCTATTGCTAAAGATGAGGTTGCTGCTTCATATCTGATTCAGGCAGAAAAAATTAAAAA
  GAAGAGAAGCACAGTCTTTGGGACTCTGCACGTTGCACACAGCTCCTCCCTAGATGAGGTAGACCACAAA
  ATTCTGGAAGCAAAGAAAGCTCTCTCTGAGTTGACAACTTGTTTACGAGAACGACTTTTTCGCTGGCAAC
  AAATTGAGAAGATCTGTGGCTTTCAGATAGCCCATAACTCAGGACTCCCCAGCCTGACCTCTTCCCTTTA
  TTCTGATCACAGCTGGGTGGTGATGCCCAGAGTCTCCATTCCACCCTATCCAATTGCTGGAGGAGTTGAT
  GACTTAGATGAAGACACACCCCCAATAGTGTCACAATTTCCCGGGACCATGGCTAAACCTCCTGGATCAT
  TAGCCAGAAGCAGCAGCCTGTGCCGTTCACGCCGCAGCATTGTGCCGTCCTCGCCTCAGCCTCAGCGAGC
  TCAGCTTGCTCCACACGCCCCCCACCCGTCACACCCTCGGCACCCTCACCACCCGCAACACACACCACAC
  TCCTTGCCTTCCCCTGATCCAGATATCCTCTCAGTGTCAAGTTGCCCTGCGCTTTATCGAAATGAAGAGG
  AGGAAGAGGCCATTTACTTCTCTGCTGAAAAGCAATGGGAAGTGCCAGACACAGCTTCAGAATGTGACTC
  CTTAAATTCTTCCATTGGAAGGAAACAGTCTCCTCCTTTAAGCCTCGAGATATACCAAACATTATCTCCG
  CGAAAGATATCAAGAGATGAGGTGTCCCTAGAGGATTCCTCCCGAGGGGATTCGCCTGTAACTGTGGATG
  TGTCTTGGGGTTCTCCCGACTGTGTAGGTCTGACAGAAACTAAGAGTATGATCTTCAGTCCTGCAAGCAA
  AGTGTACAATGGCATTTTGGAGAAATCCTGTAGCATGAACCAGCTTTCCAGTGGCATCCCGGTGCCTAAA
  CCTCGCCACACATCATGTTCCTCAGCTGGCAACGACAGTAAACCAGTTCAGGAAGCCCCAAGTGTTGCCA
  GAATAAGCAGCATCCCACATGACCTTTGTCATAATGGAGAGAAAAGCAAAAAGCCATCAAAAATCAAAAG
  CCTTTTTAAGAAGAAATCTAAGTGA
  
  Clone variation with respect to NM_001169118.1
  
• Restriction Sites: Please inquire
• ACCN: NM_001169118
• Insert Size: 3900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001169118.1, NP_001162589.1
• RefSeq Size: 5178 bp
• RefSeq ORF: 2265 bp
• Locus ID: 57620
• UniProt ID: Q9P246
• Cytogenetics: 4p15.2
• Gene Summary: This gene is a member of the stromal interaction molecule (STIM) family and likely arose, along with related family member STIM1, from a common ancestral gene. The encoded protein functions to regulate calcium concentrations in the cytosol and endoplasmic reticulum, and is involved in the activation of plasma membrane Orai Ca(2+) entry channels. This gene initiates translation from a non-AUG (UUG) start site. A signal peptide is cleaved from the resulting protein. Multiple transcript variants result from alternative splicing. [provided by RefSeq, Dec 2009]
  Transcript Variant: This variant (1, also known as STIM2.1 or STIM2beta) encodes the longest isoform (1). This isoform functions as a negative regulator of store-operated calcium entry (SOCE).