GGA1 (NM_001172687) Human Untagged Clone

CAT#: SC329014

GGA1 (untagged)-Human golgi-associated gamma adaptin ear containing ARF binding protein 1 (GGA1) transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GGA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC329014 representing NM_001172687.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGCCCGCGATGGAGCCGGAGACTCTGGAGGCGCGAATCAATAGAGCCACGAACCCCCTGAACAAG
  GAGCTCGACTGGGCCAGCATCAACGGCTTCTGCGAGCAGCTCAACGAGGACTTTGAGGGGCCTCCACTC
  GCCACCCGGCTGCTGGCCCACAAGATCCAGTCCCCACAGGAGTGGGAGGCGATCCAGGCCTTGACGGTG
  CTGGAAACATGCATGAAGAGCTGCGGCAAGCGGTTCCACGACGAAGTGGGCAAGTTCCGCTTTCTCAAC
  GAGCTCATCAAGGTCGTGTCTCCCAAGTATCTGGGCTCTCGGACATCGGAGAAGGTGAAGAACAAGATC
  TTGGAGCTCCTCTACAGCTGGACAGTGGGCCTGCCCGAGGAGGTGAAAATCGCAGAGGCCTACCAGATG
  CTAAAGAAGCAGGGGATTGTAAAGTCCGACCCCAAGCTTCCAGATGACACTACCTTTCCCCTTCCTCCT
  CCACGGCCGAAGAATGTGATCTTTGAAGATGAGGAGAAATCCAAGATGCTGGCCCGCCTGCTGAAGAGC
  TCCCATCCCGAAGACCTCCGCGCAGCCAATAAGCTCATCAAAGAGATGGTGCAGGAGGACCAGAAGCGG
  ATGGAGAAGATCTCGAAGAGGGTGAATGCCATCGAGGAGGTGAACAACAATGTGAAACTGCTCACGGAG
  ATGGTGATGAGCCACAGCCAGGGCGGCGCAGCAGCTGGCAGCAGCGAGGACCTCATGAAGGAACTGTAC
  CAGCGCTGTGAGCGGATGCGGCCCACGCTCTTCCGACTGGCGAGTGACACAGAGGACAATGATGAGGCC
  TTAGCGGAGATCCTGCAGGCCAATGACAACCTCACCCAGGTGATCAACCTGTATAAGCAGCTGGTGCGG
  GGTGAGGAGGTCAACGGTGATGCCACAGCCGGCTCCATCCCTGGGAGCACCTCGGCCCTGCTGGATCTC
  TCAGGCCTGGATCTCCCGCCTGCGGGCACCACCTACCCAGCTATGCCCACCCGCCCTGGCGAGCAGGCC
  AGCCCTGAGCAGCCCAGTGCCTCAGTTTCCCTGCTTGACGACGAGCTCATGTCTCTGGGCCTCAGTGAC
  CCCACACCCCCTTCAGGCCCAAGCCTGGATGGTACCGGATGGAACAGCTTCCAGTCGTCGGATGCCACT
  GAGCCCCCAGCCCCTGCTCTGGCCCAGGCCCCCAGTATGGAAAGCCGACCCCCAGCGCAGACATCCCTG
  CCAGCAAGCAGCGGTCTGGACGACCTAGACCTCCTGGGGAAGACCCTCCTGCAGCAGTCGCTGCCCCCG
  GAATCCCAGGAGAAGCAGCAGCCAACCCCCCGGCTCACACTCCGGGACCTGCAGAATAAGAGCAGCAGC
  TGCAGCTCCCCCAGCTCCAGCGCCACCAGCCTTCTCCACACCGTGTCCCCAGAGCCCCCCAGGCCTCCG
  CAGCAGCCCGTACCAACCGAGCTCTCACTGGCCAGCATCACTGTGCCCCTGGAGTCCATCAAACCCAGC
  AACATCCTGCCCGTGACTGTGTATGACCAGCACGGCTTCCGCATCCTCTTCCATTTTGCCCGGGACCCA
  CTGCCAGGGCGCTCCGACGTGCTGGTGGTGGTGGTTTCCATGCTGAGCACCGCCCCCCAGCCCATCCGC
  AACATCGTGTTCCAGTCAGCTGTCCCCAAGGTTATGAAGGTGAAGCTGCAGCCACCCTCGGGCACGGAG
  CTGCCAGCTTTTAACCCCATCGTCCACCCCTCAGCAATCACCCAGGTCCTGCTGCTTGCCAACCCCCAG
  AAGGAGAAGGTTCGCCTCCGCTACAAGCTCACCTTCACCATGGGTGACCAGACCTACAACGAGATGGGG
  GATGTGGACCAGTTCCCCCCACCTGAAACCTGGGGTAGCCTCTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001172687
• Insert Size: 1908 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172687.1
• RefSeq Size: 3173 bp
• RefSeq ORF: 1908 bp
• Locus ID: 26088
• UniProt ID: Q9UJY5
• Cytogenetics: 22q13.1
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome
• MW: 69.8 kDa
• Gene Summary: This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (4) lacks an in-frame segment in the coding region, as compared to variant 1. The resulting isoform (4) lacks an internal segment of 4 aa, as compared to isoform 1.