Repulsive Guidance Molecule A (RGMA) (NM_001166289) Human Untagged Clone

CAT#: SC328716

RGMA (untagged)-Human RGM domain family member A (RGMA) transcript variant 6

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RGMA
• Synonyms: RGM
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001166289, the custom clone sequence may differ by one or more nucleotides
  ATGGGTATGGGGAGAGGGGCAGGACGTTCAGCCCTGGGATTCTGGCCGACCCTCGCCTTC
  CTTCTCTGCAGCTTCCCCGCAGCCACCTCCCCGTGCAAGATCCTCAAGTGCAACTCTGAG
  TTCTGGAGCGCCACGTCGGGCAGCCACGCCCCAGCCTCAGACGACACCCCCGAGTTCTGT
  GCAGCCTTGCGCAGCTACGCCCTGTGCACGCGGCGGACGGCCCGCACCTGCCGGGGTGAC
  CTGGCCTACCACTCGGCCGTCCATGGCATAGAGGACCTCATGAGCCAGCACAACTGCTCC
  AAGGATGGCCCCACCTCGCAGCCACGCCTGCGCACGCTCCCACCGGCCGGAGACAGCCAG
  GAGCGCTCGGACAGCCCCGAGATCTGCCATTACGAGAAGAGCTTTCACAAGCACTCGGCC
  ACCCCCAACTACACGCACTGTGGCCTCTTCGGGGACCCACACCTCAGGACTTTCACCGAC
  CGCTTCCAGACCTGCAAGGTGCAGGGCGCCTGGCCGCTCATCGACAATAATTACCTGAAC
  GTGCAGGTCACCAACACGCCTGTGCTGCCCGGCTCAGCGGCCACTGCCACCAGCAAGCTC
  ACCATCATCTTCAAGAACTTCCAGGAGTGTGTGGACCAGAAGGTGTACCAGGCTGAGATG
  GACGAGCTCCCGGCCGCCTTCGTGGATGGCTCTAAGAACGGTGGGGACAAGCACGGGGCC
  AACAGCCTGAAGATCACTGAGAAGGTGTCAGGCCAGCACGTGGAGATCCAGGCCAAGTAC
  ATCGGCACCACCATCGTGGTGCGCCAGGTGGGCCGCTACCTGACCTTTGCCGTCCGCATG
  CCAGAGGAAGTGGTCAATGCTGTGGAGGACTGGGACAGCCAGGGTCTCTACCTCTGCCTG
  CGGGGCTGCCCCCTCAACCAGCAGATCGACTTCCAGGCCTTCCACACCAATGCTGAGGGC
  ACCGGTGCCCGCAGGCTGGCAGCCGCCAGCCCTGCACCCACAGCCCCCGAGACCTTCCCA
  TACGAGACAGCCGTGGCCAAGTGCAAGGAGAAGCTGCCGGTGGAGGACCTGTACTACCAG
  GCCTGCGTCTTCGACCTCCTCACCACGGGCGACGTGAACTTCACACTGGCCGCCTACTAC
  GCGTTGGAGGATGTCAAGATGCTCCACTCCAACAAAGACAAACTGCACCTGTATGAGAGG
  ACTCGGGACCTGCCAGGCAGGGCGGCTGCGGGGCTGCCCCTGGCCCCCCGGCCCCTCCTG
  GGCGCCCTCGTCCCGCTCCTGGCCCTGCTCCCTGTGTTCTGCTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_001166289
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166289.1, NP_001159761.1
• RefSeq Size: 3078 bp
• RefSeq ORF: 1305 bp
• Locus ID: 56963
• UniProt ID: Q96B86
• Cytogenetics: 15q26.1
• Gene Summary: This gene encodes a member of the repulsive guidance molecule family. The encoded protein is a glycosylphosphatidylinositol-anchored glycoprotein that functions as an axon guidance protein in the developing and adult central nervous system. This protein may also function as a tumor suppressor in some cancers. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
  Transcript Variant: This variant (6) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2, 3, 5 and 6 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.