ARL13B (NM_001174150) Human Untagged Clone

CAT#: SC328705

ARL13B (untagged)-Human ADP-ribosylation factor-like 13B (ARL13B) transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARL13B
• Synonyms: ARL2L1; JBTS8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC328705 representing NM_001174150.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTTCAGTCTGATGGCCAGTTGCTGCGGCTGGTTCAAGCGGTGGCGGGAGCCTGTCAGAAAGGTGACT
  CTTTTGATGGTGGGACTTGATAATGCTGGTAAAACCGCAACAGCAAAGGGAATCCAAGGAGAATACCCT
  GAAGATGTAGCTCCTACTGTTGGATTTTCAAAAATTAACCTTAGACAAGGAAAGTTTGAAGTCACCATC
  TTTGACTTGGGAGGTGGAATAAGAATTCGGGGAATCTGGAAGAATTACTATGCTGAATCCTATGGGGTA
  ATATTTGTTGTGGATTCCAGTGATGAAGAGAGAATGGAAGAGACAAAAGAGGCTATGTCAGAAATGCTA
  AGACATCCTAGGATATCGGGAAAGCCTATATTGGTGTTGGCAAATAAACAAGATAAAGAAGGAGCTTTA
  GGAGAAGCTGATGTCATTGAATGTCTATCTCTGGAAAAATTGGTCAATGAGCACAAGTGCCTGTGTCAG
  ATAGAACCATGTTCAGCAATCTCGGGGTATGGAAAGAAAATTGACAAGTCCATTAAAAAAGGCCTTTAT
  TGGCTGCTACATGTTATTGCAAGAGACTTTGATGCCTTAAATGAACGCATCCAAAAAGAGACAACAGAG
  CAGCGTGCTCTTGAGGAACAAGAGAAACAAGAAAGAGCTGAACGAGTGCGAAAATTACGAGAAGAAAGA
  AAACAAAATGAACAGGAGCAGGCTGAACTCGATGGAACCAGTGGTCTGGCTGAGTTGGACCCAGAACCA
  ACGAATCCTTTCCAGCCAATAGCATCTGTAATCATTGAGAATGAAGGAAAACTTGAAAGAGAGAAAAAA
  AACCAAAAAATGGAGAAAGACAGTGATGGCTGCCACCTGAAACATAAAATGGAGCATGAGCAAATAGAG
  ACACAAGGCCAGGTTAATCACAATGGCCAAAAAAATAATGAATTTGGACTAGTAGAAAATTATAAGGAG
  GCATTAACACAGCAGTTAAAGAATGAAGATGAGACAGACCGGCCATCATTGGAATCAGCTAATGGTAAA
  AAGAAAACTAAGAAACTAAGAATGAAAAGGAACCACCGGGTAGAACCACTTAATATAGATGACTGTGCT
  CCTGAGAGTCCAACGCCACCCCCACCCCCTCCTCCTGTTGGCTGGGGAACCCCTAAAGTCACTAGACTT
  CCAAAACTTGAGCCTCTTGGTGAAACACATCATAATGATTTCTATAGGAAGCCACTGCCTCCCCTGGCT
  GTGCCACAGCGACCTAACAGTGATGCTCATGATGTGATCTCATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001174150
• Insert Size: 1287 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001174150.1
• RefSeq Size: 3991 bp
• RefSeq ORF: 1287 bp
• Locus ID: 200894
• UniProt ID: Q3SXY8
• Cytogenetics: 3q11.1-q11.2
• MW: 48.6 kDa
• Gene Summary: This gene encodes a member of the ADP-ribosylation factor-like family. The encoded protein is a small GTPase that contains both N-terminal and C-terminal guanine nucleotide-binding motifs. This protein is localized in the cilia and plays a role in cilia formation and in maintenance of cilia. Mutations in this gene are the cause of Joubert syndrome 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
  Transcript Variant: This variant (3) differs in the 3' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (1).