SIGLEC6 (NM_001177547) Human Untagged Clone

CAT#: SC328642

SIGLEC6 (untagged)-Human sialic acid binding Ig-like lectin 6 (SIGLEC6) transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SIGLEC6
• Synonyms: CD33L; CD33L1; CD33L2; CD327; CDW327; OBBP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC328642 representing NM_001177547.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCAGGGAGCCCAGGAAGCCTCCGCCTCAGAGATGCTACCGCTGCTGCTGCCCCTGCTGTGGGCAGCC
  TCGTACTATGGTTATGGCTACTGGTTCCTGGAAGGGGCTGATGTTCCAGTGGCCACAAACGACCCAGAC
  GAAGAAGTGCAGGAGGAGACCCGGGGCCGATTCCACCTCCTCTGGGATCCCAGAAGGAAGAACTGCTCC
  CTGAGCATCAGAGATGCCCGGAGGAGGGACAATGCTGCATACTTCTTTCGGTTGAAGTCCAAATGGATG
  AAATACGGTTATACATCTTCCAAGCTCTCTGTGCGTGTGATGGCCCTGACCCACAGGCCCAACATCTCC
  ATCCCAGGGACCCTGGAGTCTGGCCATCCCAGCAATCTGACCTGCTCTGTGCCCTGGGTCTGTGAGCAG
  GGGACGCCCCCCATCTTCTCCTGGATGTCAGCTGCCCCCACCTCCCTGGGCCCCAGGACCACCCAGTCC
  TCGGTGCTCACAATCACCCCACGGCCCCAGGACCACAGCACCAACCTCACCTGTCAGGTGACGTTCCCT
  GGAGCCGGTGTGACCATGGAGAGAACCATCCAGCTCAATGTCTCCTCCTTCAAAATCCTGCAAAACACC
  TCGTCCCTCCCTGTCCTGGAGGGCCAGGCTCTGCGGCTGCTCTGTGATGCTGACGGCAACCCCCCTGCA
  CACCTGAGCTGGTTCCAGGGCTTCCCCGCCCTGAACGCCACCCCCATCTCCAATACCGGGGTCCTGGAG
  CTGCCTCAAGTAGGGTCTGCAGAAGAAGGAGATTTCACCTGCCGTGCTCAGCATCCTCTGGGCTCCCTG
  CAAATCTCTCTGAGTCTCTTTGTGCATTGGAAACCAGAAGGCAGGGCTGGTGGTGTCCTGGGAGCAGTC
  TGGGGAGCTAGCATCACAACCCTGGTTTTCCTCTGTGTTTGCTTCATCTTCAGAGTGAAGACTAGAAGG
  AAGAAAGCAGCCCAGCCAGTGCAAAACACGGATGATGTGAACCCCGTCATGGTCTCAGGCTCCAGGGGT
  CATCAGCACCAGTTCCAGACAGGCATAGTTTCAGACCACCCTGCTGAGGCTGGCCCCATCTCAGAAGAT
  GAGCAGGAGCTCCACTACGCTGTCCTACACTTCCACAAGGTGCAACCTCAGGAACCAAAGGTCACCGAC
  ACTGAGTACTCAGAAATCAAGATACACAAGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001177547
• Insert Size: 1206 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177547.2
• RefSeq Size: 3707 bp
• RefSeq ORF: 1206 bp
• Locus ID: 946
• UniProt ID: O43699
• Cytogenetics: 19q13.41
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• MW: 44.3 kDa
• Gene Summary: This gene encodes a member of the SIGLEC (sialic acid binding immunoglobulin-like lectin) family of proteins. The encoded transmembrane receptor binds sialyl-TN glycans and leptin. Placental expression of the encoded protein is upregulated in preeclampsia. [provided by RefSeq, Jul 2016]
  Transcript Variant: This variant (4) uses an alternate, in-frame splice site and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 4), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.