Adiponectin (ADIPOQ) (NM_001177800) Human Untagged Clone

CAT#: SC328394

ADIPOQ (untagged)-Human adiponectin C1Q and collagen domain containing (ADIPOQ) transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Adiponectin
• Synonyms: ACDC; ACRP30; ADIPQTL1; ADPN; APM-1; APM1; GBP28
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001177800 edited
  GCCATCTCCTCCTCACTTCCATTCTGACTGCAGTCTGTGGTTCTGATTCCATACCAGAGG
  GGCTCAGGATGCTGTTGCTGGGAGCTGTTCTACTGCTATTAGCTCTGCCCGGTCATGACC
  AGGAAACCACGACTCAAGGGCCCGGAGTCCTGCTTCCCCTGCCCAAGGGGGCCTGCACAG
  GTTGGATGGCGGGCATCCCAGGGCATCCGGGCCATAATGGGGCCCCAGGCCGTGATGGCA
  GAGATGGCACCCCTGGTGAGAAGGGTGAGAAAGGAGATCCAGGTCTTATTGGTCCTAAGG
  GAGACATCGGTGAAACCGGAGTACCCGGGGCTGAAGGTCCCCGAGGCTTTCCGGGAATCC
  AAGGCAGGAAAGGAGAACCTGGAGAAGGTGCCTATGTATACCGCTCAGCATTCAGTGTGG
  GATTGGAGACTTACGTTACTATCCCCAACATGCCCATTCGCTTTACCAAGATCTTCTACA
  ATCAGCAAAACCACTATGATGGCTCCACTGGTAAATTCCACTGCAACATTCCTGGGCTGT
  ACTACTTTGCCTACCACATCACAGTCTATATGAAGGATGTGAAGGTCAGCCTCTTCAAGA
  AGGACAAGGCTATGCTCTTCACCTATGATCAGTACCAGGAAAATAATGTGGACCAGGCCT
  CCGGCTCTGTGCTCCTGCATCTGGAGGTGGGCGACCAAGTCTGGCTCCAGGTGTATGGGG
  AAGGAGAGCGTAATGGACTCTATGCTGATAATGACAATGACTCCACCTTCACAGGCTTTC
  TTCTCTACCATGACACCAACTGATCACCACTAACTCAGAGCCTCCTCCAGGCCAAACAGC
  CCCAAAGTCAATTAAAGGCTTTCAGTACGGTTAGGAAGTTGATTATTATTTAGTTGGAGG
  CCTTTAGATATTATTCATTCATTTACTCATTCATTTATTCATTCATTCATCAAGTAACTT
  TAAAAAAATCATATGCTATGTTCCCAGTCCTGGGGAGCTTCACAAACATGACCAGATAAC
  TGACTAGAAAGAAGTAGTTGACAGTGCTATTTTGTGCCCACTGTCTCTCCTGATGCTCAT
  ATCAATCCTATAAGGCACAGGGAACAAGCATTCTCCTGTTTTTACAGATTGTATCCTGAG
  GCTGAGAGAGTTAAGTGAATGTCTAAGGTCACACAAGTATTAAGTGACAGTGCTAGAAAT
  CAAACCCAGAGCTGTGGACTTTGTTCACTAGACTGTGCCCTTTTATAGAGGTACATGTTC
  TCTTTGGAGTGTTGGTAGGTGTCTGTTTCCCACCTCACCTGAGAGCCATTGAATTTGCCT
  TCCTCATGAATTAAAACCTCCCCCAAGCAGAGCTTCCTCAGAGAAAGTGGTTCTATGATG
  AAGTCCTGTCTTGGAAGGACTACTACTCAATGGCCCCTGCACTACTCTACTTCCTCTTAC
  CTATGTCCCTTCTCATGCCTTTCCCTCCAACGGGGAAAGCCAACTCCATCTCTAAGTGCT
  GAACTCATCCCTGTTCCTCAAGGCCACCTGGCCAGGAGCTTCTCTGATGTGATATCCACT
  TTTTTTTTTTTTTGAGATGGAGTCTCACTCTGTCACCCAGGCTGGAGTACAGTGACACGA
  CCTCGGCTCACTGCAGCCTCCTTCTCCTGGGTCCAAGCAATTATTGTGCCTCAGCCTCCC
  GAGTAGCTGAGACTTCAGGTGCATTCCACCACACATGGCTAATTTTTGTATTTTTAGTAG
  AAATGGGGTTTCGTCATGTTGGCCAGGCTGGTCTCGAACTCCTGGCCTAGGTGATCCACC
  CGCCTCGACCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCATGCCCAGTCGATATC
  TCACTTTTTATTTTGCCATGGATGAGAGTCCTGGGTGTGAGGAACACCTCCCACCAGGCT
  AGAGGCAACTGCCCAGGAAGGACTGTGCTTCCGTCACCTCTAAATCCCTTGCAGATCCTT
  GATAAATGCCTCATGAAGACCAATCTCTTGAATCCCGTATCTACCCAGAATTAACTCCAT
  TCCAGTCTCTGCATGTAATCAGTTTTATCCACAGAAACATTTTCATTTTAGGAAATCCCT
  GGTTTTAAGTATCAATCCTTGTTCAGCTGGACAATATGAATCTTTTCCACTGAAGTTAGG
  GATGACTGTGATTTTCAGAACACGTCCAGAATTTTTCATCAAGAAGGTAGCTTGAGCCTG
  AAATGCAAAACCCATGGAGGAATTC
  
• Restriction Sites: Please inquire
• ACCN: NM_001177800
• Insert Size: 2200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177800.1, NP_001171271.1
• RefSeq Size: 4629 bp
• RefSeq ORF: 735 bp
• Locus ID: 9370
• UniProt ID: Q15848
• Cytogenetics: 3q27.3
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Adipocytokine signaling pathway, PPAR signaling pathway, Type II diabetes mellitus
• Gene Summary: This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.