KIF7 (NM_198525) Human Untagged Clone

CAT#: SC328068

KIF7 (untagged)-Human kinesin family member 7 (KIF7)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KIF7
• Synonyms: ACLS; AGBK; HLS2; JBTS12; UNQ340
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC328068 representing NM_198525.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGGCTGGAGGCTCAGAGGCTGCCAGGGGCTGAGGAGGCCCCAGTGCGGGTTGCCCTGCGAGTTCGA
  CCACTGCTGCCCAAGGAGCTGCTGCACGGGCATCAGAGCTGCCTGCAGGTGGAGCCAGGGCTTGGCCGC
  GTCACTCTGGGCCGTGACCGACACTTTGGCTTCCACGTGGTGCTGGCCGAGGATGCGGGGCAGGAGGCC
  GTGTACCAGGCCTGCGTTCAGCCCCTCCTTGAGGCCTTCTTCGAGGGCTTCAATGCCACTGTCTTTGCC
  TATGGTCAGACGGGCTCAGGGAAGACATACACCATGGGGGAGGCCAGTGTGGCCTCCCTCCTTGAGGAT
  GAGCAGGGCATTGTCCCGAGGGCCATGGCCGAGGCCTTCAAGCTCATCGATGAGAACGACCTGCTTGAC
  TGTCTGGTACATGTGTCCTACCTGGAAGTGTACAAGGAGGAGTTCCGAGACCTGCTCGAGGTGGGCACT
  GCCAGCCGTGACATCCAGCTCCGGGAAGATGAGCGCGGGAATGTTGTGCTGTGCGGGGTGAAGGAGGTC
  GACGTGGAGGGCCTGGATGAGGTGCTGAGCCTCCTGGAGATGGGCAACGCGGCGCGGCACACGGGAGCC
  ACGCACCTCAACCACCTGTCTAGCCGCTCACACACGGTCTTCACCGTGACCCTGGAGCAGCGGGGGCGC
  GCCCCCAGCCGCCTACCCCGCCCCGCCCCGGGCCAGCTGCTCGTCTCCAAGTTCCACTTCGTGGACCTG
  GCGGGCTCAGAGAGGGTGCTCAAGACGGGCAGCACCGGCGAGCGGCTCAAGGAGAGCATCCAGATCAAC
  AGCAGCCTCCTGGCGCTGGGCAACGTCATCAGCGCCCTGGGGGACCCTCAGCGCCGGGGCAGCCACATA
  CCCTACCGCGACTCCAAGATCACCCGGATCCTCAAAGACTCGCTGGGCGGGAACGCCAAGACGGTGATG
  ATCGCCTGCGTCAGCCCTTCCTCCTCCGACTTCGACGAGACCCTCAACACCCTCAACTACGCCAGCCGC
  GCCCAGAACATCCGCAACCGCGCCACGGTCAACTGGCGGCCCGAGGCCGAGCGGCCACCCGAAGAGACG
  GCGAGCGGCGCGCGGGGTCCGCCACGGCACCGCTCCGAGACCCGCATCATCCACCGCGGCCGGCGCGCC
  CCAGGCCCAGCCACCGCCTCCGCGGCGGCCGCCATGCGCCTGGGCGCCGAGTGCGCGCGCTACCGGGCC
  TGCACCGACGCCGCCTACAGCCTCTTGCGCGAGCTGCAGGCCGAGCCCGGGCTGCCCGGCGCCGCCGCC
  CGCAAGGTGCGCGACTGGCTGTGCGCCGTCGAGGGCGAGCGCAGCGCCCTGAGCTCCGCCTCCGGGCCC
  GATAGCGGCATCGAGAGCGCCTCCGTCGAGGACCAGGCGGCGCAGGGGGCCGGCGGGCGAAAGGAGGAT
  GAGGGGGCGCAGCAGCTGCTGACCCTGCAGAACCAGGTGGCGCGGCTGGAGGAGGAGAACCGAGACTTT
  CTGGCTGCGCTGGAGGACGCCATGGAGCAGTACAAACTGCAGAGCGACCGGCTGCGTGAGCAGCAGGAG
  GAGATGGTGGAACTGCGGCTGCGGTTAGAGCTGGTGCGGCCAGGCTGGGGGGGCCCGCGGCTCCTGAAT
  GGCCTGCCTCCCGGGTCCTTTGTGCCTCGACCTCATACAGCCCCCCTGGGGGGTGCCCACGCCCATGTG
  CTGGGCATGGTGCCGCCTGCCTGCCTCCCTGGAGATGAAGTTGGCTCTGAGCAGAGGGGAGAGCAGGTG
  ACAAATGGCAGGGAGGCTGGAGCTGAGTTGCTGACTGAGGTGAACAGGCTGGGAAGTGGCTCTTCAGCT
  GCTTCAGAGGAGGAAGAGGAGGAGGAGGAGCCGCCCAGGCGGACCTTACACCTGCGCAGAAATAGGATC
  AGCAACTGCAGTCAGAGGGCGGGGGCACGCCCAGGGAGTCTGCCAGAGAGGAAGGGCCCAGAGCTTTGC
  CTTGAGGAGTTGGATGCAGCCATTCCAGGGTCCAGAGCAGTTGGTGGGAGCAAGGCCCGAGTTCAGGCC
  CGCCAGGTCCCCCCTGCCACAGCCTCAGAGTGGCGGCTGGCCCAGGCCCAGCAGAAGATCCGGGAGCTG
  GCTATCAACATCCGCATGAAGGAGGAGCTTATTGGCGAGCTGGTCCGCACAGGAAAGGCAGCTCAGGCC
  CTGAACCGCCAGCACAGCCAGCGTATCCGGGAGCTGGAGCAGGAGGCAGAGCAGGTGCGGGCCGAGCTG
  AGTGAAGGCCAGAGGCAGCTGCGGGAGCTCGAGGGCAAGGAGCTCCAGGATGCTGGCGAGCGGTCTCGG
  CTCCAGGAGTTCCGCAGGAGGGTCGCTGCGGCCCAGAGCCAGGTGCAGGTGCTGAAGGAGAAGAAGCAG
  GCTACGGAGCGGCTGGTGTCACTGTCGGCCCAGAGTGAGAAGCGACTGCAGGAGCTCGAGCGGAACGTG
  CAGCTCATGCGGCAGCAGCAGGGACAGCTGCAGAGGCGGCTTCGCGAGGAGACGGAGCAGAAGCGGCGC
  CTGGAGGCAGAAATGAGCAAGCGGCAGCACCGCGTCAAGGAGCTGGAGCTGAAGCATGAGCAACAGCAG
  AAGATCCTGAAGATTAAGACGGAAGAGATCGCGGCATTCCAGAGGAAGAGGCGCAGTGGCAGCAACGGC
  TCTGTGGTCAGCCTGGAACAGCAGCAGAAGATTGAGGAGCAGAAGAAGTGGCTGGACCAGGAGATGGAG
  AAGGTGCTACAGCAGCGGCGGGCGCTGGAGGAGCTGGGGGAGGAGCTCCACAAGCGGGAGGCCATCCTG
  GCCAAGAAGGAGGCCCTGATGCAGGAGAAGACGGGGCTGGAGAGCAAGCGCCTGAGATCCAGCCAGGCC
  CTCAACGAGGACATCGTGCGAGTGTCCAGCCGGCTGGAGCACCTGGAGAAGGAGCTGTCCGAGAAGAGC
  GGGCAGCTGCGGCAGGGCAGCGCCCAGAGCCAGCAGCAGATCCGCGGGGAGATCGACAGCCTGCGCCAG
  GAGAAGGACTCGCTGCTCAAGCAGCGCCTGGAGATCGACGGCAAGCTGAGGCAGGGGAGTCTGCTGTCC
  CCCGAGGAGGAGCGGACGCTGTTCCAGTTGGATGAGGCCATCGAGGCCCTGGATGCTGCCATTGAGTAT
  AAGAATGAGGCCATCACATGCCGCCAGCGGGTGCTTCGGGCCTCAGCCTCGTTGCTGTCCCAGTGCGAG
  ATGAACCTCATGGCCAAGCTCAGCTACCTCTCATCCTCAGAGACCAGAGCCCTCCTCTGCAAGTATTTT
  GACAAGGTGGTGACGCTCCGAGAGGAGCAGCACCAGCAGCAGATTGCCTTCTCGGAACTGGAGATGCAG
  CTGGAGGAGCAGCAGAGGCTGGTGTACTGGCTGGAGGTGGCCCTGGAGCGGCAGCGCCTGGAGATGGAC
  CGCCAGCTGACCCTGCAGCAGAAGGAGCACGAGCAGAACATGCAGCTGCTCCTGCAGCAGAGTCGAGAC
  CACCTCGGTGAAGGGTTAGCAGACAGCAGGAGGCAGTATGAGGCCCGGATTCAAGCTCTGGAGAAGGAA
  CTGGGCCGTTACATGTGGATAAACCAGGAACTGAAACAGAAGCTCGGCGGTGTGAACGCTGTAGGCCAC
  AGCAGGGGTGGGGAGAAGAGGAGCCTGTGCTCGGAGGGCAGACAGGCTCCTGGAAATGAAGATGAGCTC
  CACCTGGCACCCGAGCTTCTCTGGCTGTCCCCCCTCACTGAGGGGGCCCCCCGCACCCGGGAGGAGACG
  CGGGACTTGGTCCACGCTCCGTTACCCTTGACCTGGAAACGCTCGAGCCTGTGTGGTGAGGAGCAGGGG
  TCCCCCGAGGAACTGAGGCAGCGGGAGGCGGCTGAGCCCCTGGTGGGGCGGGTGCTTCCTGTGGGTGAG
  GCAGGCCTGCCCTGGAACTTTGGGCCTTTGTCCAAGCCCCGGCGGGAACTGCGACGAGCCAGCCCGGGG
  ATGATTGATGTCCGGAAAAACCCCCTGTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_198525
• Insert Size: 4032 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198525.2
• RefSeq Size: 4575 bp
• RefSeq ORF: 4032 bp
• Locus ID: 374654
• UniProt ID: Q2M1P5
• Cytogenetics: 15q26.1
• Protein Families: Druggable Genome
• MW: 150.6 kDa
• Gene Summary: This gene encodes a cilia-associated protein belonging to the kinesin family. This protein plays a role in the sonic hedgehog (SHH) signaling pathway through the regulation of GLI transcription factors. It functions as a negative regulator of the SHH pathway by preventing inappropriate activation of GLI2 in the absence of ligand, and as a positive regulator by preventing the processing of GLI3 into its repressor form. Mutations in this gene have been associated with various ciliopathies. [provided by RefSeq, Oct 2011]