C9orf86 (RABL6) (NM_024718) Human Untagged Clone

CAT#: SC327988

RABL6 (untagged)-Human chromosome 9 open reading frame 86 (C9orf86) transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: C9orf86
• Synonyms: C9orf86; PARF; pp8875; RBEL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC327988 representing NM_024718.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTTTTCCGCCCTGAAGAAGCTGGTGGGGTCGGACCAGGCCCCGGGCCGGGACAAGAACATCCCCGCC
  GGGCTGCAGTCCATGAACCAGGCGTTGCAGAGGCGCTTCGCCAAGGGGGTGCAGTACAACATGAAGATA
  GTGATCCGGGGAGACAGGAACACGGGCAAGACAGCGCTGTGGCACCGCCTGCAGGGCCGGCCGTTCGTG
  GAGGAGTACATCCCCACACAGGAGATCCAGGTCACCAGCATCCACTGGAGCTACAAGACCACGGATGAC
  ATCGTGAAGGTTGAAGTCTGGGATGTAGTAGACAAAGGAAAATGCAAAAAGCGAGGCGACGGCTTAAAG
  ATGGAGAACGACCCCCAGGAGGCGGAGTCTGAAATGGCCCTGGATGCTGAGTTCCTGGACGTGTACAAG
  AACTGCAACGGGGTGGTCATGATGTTCGACATTACCAAGCAGTGGACCTTCAATTACATTCTCCGGGAG
  CTTCCAAAAGTGCCCACCCACGTGCCAGTGTGCGTGCTGGGAAACTACCGGGACATGGGCGAGCACCGA
  GTCATCCTGCCGGACGACGTGCGTGACTTCATCGACAACCTGGACAGACCTCCAGGTTCCTCCTACTTC
  CGCTATGCTGAGTCTTCCATGAAGAACAGCTTCGGCCTAAAGTACCTTCATAAGTTCTTCAATATCCCA
  TTTTTGCAGCTTCAGAGGGAGACGCTGTTGCGGCAGCTGGAGACGAACCAGCTGGACATGGACGCCACG
  CTGGAGGAGCTGTCGGTGCAGCAGGAGACGGAGGACCAGAACTACGGCATCTTCCTGGAGATGATGGAG
  GCTCGCAGCCGTGGCCATGCGTCCCCACTGGCGGCCAACGGGCAGAGCCCATCCCCGGGCTCCCAGTCA
  CCAGTGGTGCCTGCAGGCGCTGTGTCCACGGGGAGCTCCAGCCCCGGCACACCCCAGCCCGCCCCACAG
  CTGCCCCTCAATGCCGCCCCACCATCCTCTGTGCCCCCTGTACCACCCTCAGAGGCCCTGCCCCCACCT
  GCGTGCCCCTCAGCCCCCGCCCCACGGCGCAGCATCATCTCTAGGCTGTTTGGGACGTCACCTGCCACC
  GAGGCAGCCCCTCCACCTCCAGAGCCAGTCCCGGCCGCAGAGGGCCCAGCAACGGTCCAGAGTGTGGAG
  GACTTTGTTCCTGACGACCGCCTGGACCGCAGCTTCCTGGAAGACACAACCCCCGCCAGGGACGAGAAG
  AAGGTGGGGGCCAAGGCTGCCCAGCAGGACAGCGACAGTGATGGGGAGGCCCTGGGCGGCAACCCGATG
  GTGGCAGGGTTCCAGGACGATGTGGACCTCGAAGACCAGCCACGTGGGAGTCCCCCGCTGCCTGCAGGC
  CCCGTCCCCAGTCAAGACATCACTCTTTCGAGTGAGGAGGAAGCAGAAGTGGCAGCTCCCACAAAAGGC
  CCTGCCCCAGCTCCCCAGCAGTGCTCAGAGCCAGAGACCAAGTGGTCCTCCATACCAGCTTCGAAGCCA
  CGGAGGGGGACAGCTCCCACGAGGACCGCAGCACCCCCCTGGCCAGGCGGTGTCTCTGTTCGCACAGGT
  CCGGAGAAGCGCAGCAGCACCAGGCCCCCTGCTGAGATGGAGCCGGGGAAGGGTGAGCAGGCCTCCTCG
  TCGGAGAGTGACCCCGAGGGACCCATTGCTGCACAAATGCTGTCCTTCGTCATGGATGACCCCGACTTT
  GAGAGCGAGGGATCAGACACACAGCGCAGGGCGGATGACTTTCCCGTGCGAGATGACCCCTCCGATGTG
  ACTGACGAGGATGAGGGCCCTGCCGAGCCGCCCCCACCCCCCAAGCTCCCTCTCCCCGCCTTCAGACTG
  AAGAATGACTCGGACCTCTTCGGGCTGGGGCTGGAGGAGGCCGGACCCAAGGAGAGCAGTGAGGAAGGT
  AAGGAGGGCAAAACCCCCTCTAAGGAGAAGAAGAAGAAGAAGAAAAAAGGCAAAGAGGAAGAAGAAAAA
  GCTGCCAAGAAGAAGAGCAAACACAAGAAGAGCAAGGACAAGGAGGAGGGCAAGGAGGAGCGGCGACGG
  CGGCAGCAGCGGCCCCCGCGCAGCAGGGAGAGGACGGCTGCCGATGAGCTGGAGGCTTTCCTGGGGGGC
  GGGGCCCCGGGCGGCCGCCACCCTGGGGGTGGCGACTACGAGGAGCTCTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_024718
• Insert Size: 2190 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024718.4
• RefSeq Size: 3148 bp
• RefSeq ORF: 2190 bp
• Locus ID: 55684
• UniProt ID: Q3YEC7
• Cytogenetics: 9q34.3
• MW: 79.5 kDa
• Gene Summary: This gene encodes a member of the Ras superfamily of small GTPases. The encoded protein binds to both GTP and GDP and may play a role in cell growth and survival. Overexpression of this gene may play a role in breast cancer tumorigenesis, and pseudogenes of this gene are located on the long arm of chromosome 2 and the short arm of chromosome 18. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
  Transcript Variant: This variant (1) uses an alternate in-frame splice site in the coding region, compared to variant 3. The resulting isoform (1) lacks one internal residue, compared to isoform 3.