Synaptopodin (SYNPO) (NM_001166208) Human Untagged Clone

CAT#: SC327251

SYNPO (untagged)-Human synaptopodin (SYNPO) transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Synaptopodin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC327251 representing NM_001166208.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTGGGTCCTCACCTCCCACCTCCGCCCCTTGCACCCAGCGAAGGGAGGCCTACCCCCTGCGCCTTC
  CAGATCCCTGATGGAAGCTACAGGTGTCTGGCTCTGGAAGCCGAGGAGAGCAGCGGTGAGGAGGGCCTG
  CAGGGAGAGGTGGGGCCTACCGACCTGGAAGAGGATGAGGGGGTCAGCAGGAGTGGGGACGACTCTGCC
  TGCAGAGTCACCCAGGGGACACCGCAGCTGCCCAAAGCCCTGGGCATCCAACCACCCAGCTGCTCCAGA
  GAGGAACAAGGGGCGTCCCAGCACGACGACAGGGCCAGCCAGGACTGGGATGTAGTGAAGGCCGGGCAG
  ATGATGACAGCCAGCCCCAGCCCTGGCCCTGGGCCCAGAGTGGCCCAGAAACCAGCGTTGGGCCGGAGC
  ACTAGCCTCACGGAGAAGGATCTGAAAGAAGCCAAGGCGCGGAGCCAGCAGATTGCAGCCCAGCTGACC
  ACCCCTCCCAGCTCCAATTCCCGTGGCGTCCAGCTCTTCAACAGGCGCCGGCAGAGGGTGAACGAGTTC
  ACCTTGGAGAGCCACGGCCAGAGGGGACAGAAGCCCAGCCAGGAGTCCCTCAGAGTGCTCCCTTCAAGC
  CTCCCAGGCCATGCACCGGGGCTCAGCCTGAGTTCCACCTCGCTGCCGGAGCCAGGCCCTCCACGGCAC
  CCCAGTCCCCAGAGCCCCGACAGAGGGGTCCCTGGCCACAGCATGGAGGGGTACTCAGAGGAGGCTAGC
  TTGCTGCGGCACCTGGAGAAGGTGGCCAGTGAGGAGGAAGAGGTACCACTGGTGGTTTATCTAAAGGAG
  AATGCAGCACTGCTGACAGCCAATGGGCTGCACCTGTCCCAAAACCGAGAGGCCCAGCAGTCCTCACCG
  GCCCCACCTCCAGCTGAGGTCCACAGCCCAGCTGCAGATGTCAACCAAAACCTTGCCTCGCCCAGTGCC
  ACGCTCACCACACCAACTTCTAACAGCAGCCACAATCCGCCAGCCACCGATGTCAATCAGAACCCACCG
  GCAACTGTTGTCCCACAGAGCCTGCCACTTTCTAGCATCCAACAGAATTCCTCAGAGGCCCAACTCCCA
  TCTAATGGCACAGGGCCTGCTTCCAAACCCAGCACCCTGTGTGCTGATGGGCAACCCCAGGCACCGGCT
  GAGGAGGTGAGATGCAGCACACTCCTAATTGACAAGGTATCAACTCCAGCTACCACCACCAGCACCTTC
  TCCAGAGAAGCTACGCTCATCCCCAGCTCCAGGCCCCCAGCCTCAGATTTCATGTCCAGCTCCCTGCTC
  ATTGACATCCAGCCCAACACCCTAGTGGTGTCAGCAGATCAAGAGATGTCTGGGCGAGCAGCTGCCACC
  ACGCCCACCAAGGTCTACAGTGAGGTCCACTTCACACTGGCCAAGCCCCCATCAGTGGTCAACAGGACG
  GCCAGGCCTTTTGGGATCCAGGCGCCAGGGGGCACCAGCCAGATGGAGAGGAGCCCCATGCTAGAGAGA
  CGACATTTTGGGGAGAAGGCCCCGGCTCCCCAGCCCCCCAGTTTGCCAGACAGGAGCCCCCGGCCACAG
  AGACACATAATGTCCCGCAGCCCCATGGTGGAAAGGAGGATGATGGGGCAGCGAAGCCCGGCCTCAGAG
  AGACGCCCCTTGGGGAACTTCACTGCACCCCCCACCTACACTGAGACCTTGTCCACAGCCCCTCTGGCT
  TCCTGGGTGAGGTCTCCTCCCTCATATTCTGTCCTGTATCCCAGCTCCGACCCCAAGTCTTCTCATCTG
  AAGGGCCAGGCGGTTCCTGCCAGCAAGACGGGCATTCTGGAGGAGTCGATGGCCCGCCGGGGCAGCCGC
  AAATCCATGTTTACTTTCGTGGAGAAGCCCAAGGTGACCCCGAATCCAGACTTGCTGGATCTGGTACAG
  ACAGCGGATGAGAAGCGGCGGCAGAGGGACCAGGGGGAGGTAGGCGTGGAGGAGGAGCCCTTCGCACTG
  GGGGCCGAGGCCTCCAACTTCCAGCAGGAGCCAGCACCTCGTGACAGGGCCAGCCCCGCGGCGGCGGAG
  GAGGTGGTACCAGAGTGGGCCTCCTGCCTCAAGTCACCCCGCATCCAGGCCAAGCCGAAGCCCAAACCC
  AACCAGAACCTCTCCGAGGCCTCTGGGAAGGGAGCTGAGCTCTACGCCCGCCGCCAGTCACGGATGGAG
  AAATATGTCATCGAGTCTTCAAGCCACACGCCAGAGCTGGCCCGCTGCCCATCACCTACCATGTCCCTG
  CCTTCCTCCTGGAAATACCCCACTAACGCCCCCGGGGCCTTCCGAGTGGCATCCCGAAGCCCAGCCCGG
  ACCCCGCCTGCCTCCCTCTACCATGGCTACCTGCCTGAGAACGGGGTCCTGCGCCCAGAGCCCACCAAG
  CAGCCGCCATACCAGCTGCGCCCCTCGCTCTTTGTCCTCTCACCTATCAAGGAGCCTGCCAAGGTCTCA
  CCAAGAGCTGCCTCGCCCGCCAAGCCCAGCTCCTTGGACCTGGTGCCCAACCTGCCCAAGGGGGCTCTC
  CCTCCATCTCCTGCCCTGCCTCGGCCCTCGCGCTCCTCACCGGGCCTCTACACCTCCCCCGGCCAGGAC
  AGCCTGCAGCCCACTGCCGTGAGCCCTCCTTACGGCGGTGACATCTCCCCCGTGTCTCCCTCCAGGGCG
  TGGTCTCCCCGAGCCAAGCAGGCCCCCAGGCCCTCCTTCTCTACCCGGAACGCCGGGATCGAGGCTCAG
  GTGTGGAAGCCTTCCTTCTGCTTCAAGTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001166208
• Insert Size: 2790 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166208.1
• RefSeq Size: 5181 bp
• RefSeq ORF: 2790 bp
• Locus ID: 11346
• UniProt ID: Q8N3V7
• Cytogenetics: 5q33.1
• MW: 99.5 kDa
• Gene Summary: Synaptopodin is an actin-associated protein that may play a role in actin-based cell shape and motility. The name synaptopodin derives from the protein's associations with postsynaptic densities and dendritic spines and with renal podocytes (Mundel et al., 1997 [PubMed 9314539]).[supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (3) has multiple differences compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at an alternate start codon, compared to variant 1. The encoded protein (isoform C) is longer than isoform 1 and contains unique N- and C-termini. Both variants 3 and 4 encode the same isoform (C).