ADCK4 (COQ8B) (NM_024876) Human Untagged Clone

CAT#: SC323680

ADCK4 (untagged)-Kinase deficient mutant (K237M) of Human aarF domain containing kinase 4 (ADCK4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADCK4
• Synonyms: ADCK4; NPHS9
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_024876, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGGCTGAAGGTGGGGGGCCTACTTCGGGGGACCGGTGGACAGCTGGGCCAGACTGTTGGTTGGCCTT
  GTGGGGCCCTGGGGCCTGGGCCCCACCGCTGGGGACCATGTGGAGGTTCTTGGGCCCAAAAGTTTTACCA
  GGATGGGCCTGGGAGAGGCCTGGGTGAGGAGGACATTCGCAGGGCACGGGAGGCCCGTCCCAGGAAGACA
  CCCCGGCCCCAGCTGAGTGACCGCTCTCGAGAACGCAAGGTGCCTGCCTCCCGCATCAGCCGCTTGGCCA
  ACTTTGGGGGACTGGCTGTGGGCTTGGGGCTAGGAGTACTGGCCGAGATGGCTAAGAAGTCCATGCCAGG
  AGGTCGTCTGCAGTCAGAGGGTGGTTCTGGGCTGGACTCCAGCCCCTTCCTGTCGGAGGCCAATGCCGAG
  CGGATTGTGCAGACCTTATGTACAGTTCGAGGGGCCGCCCTCAAGGTTGGCCAGATGCTCAGCATCCAGG
  ACAACAGCTTCATCAGCCCTCAGCTGCAGCACATCTTTGAGCGGGTCCGCCAGAGCGCCGACTTCATGCC
  CCGCTGGCAGATGCTGAGAGTTCTTGAAGAGGAGCTCGGCAGGGACTGGCAGGCCAAGGTGGCCTCCTTG
  GAGGAGGTGCCCTTTGCCGCTGCCTCAATTGGGCAGGTGCACCAGGGCCTGCTGAGGGACGGGACGGAGG
  TGGCCGTGAAGATCCAGTACCCCGGCATAGCCCAGAGCATTCAGAGCGATGTCCAGAACCTGCTGGCGGT
  ACTCAAGATGAGCGCGGCCCTGCCCGCGGGCCTGTTTGCCGAGCAGAGCCTGCAGGCCTTGCAGCAGGAG
  CTGGCTTGGGAGTGTGACTACCGTCGTGAGGCGGCTTGTGCCCAGAATTTCAGGCAGCTGCTGGCAAATG
  ACCCCTTCTTCCGGGTCCCAGCCGTGGTTAAGGAGCTGTGCACGACACGGGTGCTGGGCATGGAGCTGGC
  TGGAGGGGTCCCCCTGGACCAGTGCCAGGGCCTAAGCCAGGACCTGCGGAACCAGATTTGCTTCCAGCTC
  CTGACGCTGTGTCTGCGGGAGCTGTTTGAGTTCCGATTCATGCAGACTGACCCCAACTGGGCCAACTTCC
  TGTATGATGCCTCCAGCCACCAGGTGACCCTGCTGGACTTTGGTGCAAGCCGGGAGTTTGGGACAGAGTT
  CACAGACCATTACATCGAGGTGGTGAAGGCTGCAGCTGATGGAGACAGAGACTGTGTCCTGCAGAAGTCC
  AGGGACCTCAAATTCCTCACAGGCTTTGAAACCAAGGCATTCTCCGACGCCCACGTGGAGGCAGTGATGA
  TCCTGGGGGAGCCTTTCGCCACCCAGGGCCCTTATGACTTTGGGTCGGGGGAAACGGCCCGCCGCATACA
  GGACCTCATCCCGGTGCTGCTGCGGCACCGGCTGTGTCCCCCACCCGAGGAGACCTATGCCCTGCACCGC
  AAGCTGGCAGGGGCTTTCCTGGCCTGTGCCCACCTCCGAGCCCACATCGCCTGCAGGGACCTCTTCCAGG
  ACACCTACCACCGCTACTGGGCCAGTCGCCAGCCAGACGCAGCCACTGCCGGCAGCCTCCCCACCAAAGG
  GGACTCCTGGGTGGATCCCTCATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_024876 unedited
  CCCGCCCGTTCCAGCAAAGGGCGGTAGGCGTGTACGGTTGGGAGGTTCTATATAAGCAGAGCTCGTTTAG
  TGAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGAGCGGGA
  AACAGTTGGCAAGCAAGGCAATGTGGCTGAAGGTGGGGGGCCTACTTCGGGGGACCGGTGGACAGCTGGG
  CCAGACTGTTGGTTGGCCTTGTGGGGCCCTGGGGCCTGGGCCCCACCGCTGGGGACCATGTGGAGGTTCT
  TGGGCCCAAAAGTTTTACCAGGATGGGCCTGGGGAGAGGCCTGGGGTGAAGGAGGGACATTTCGGCAAGG
  GCACGGGAGAGGCCCGGTCCAAGGAAAGAACACCCCCGGCCCCCACTCTGATGTGACCCGCTCTCTCGAA
  AACGCCAAGTGTGCCGGCCCTCCCCCCATCAGCCCCCTTGCCACACCTTGGGGGGGACTGCCTGGGGGGC
  TGGGGGGCTAGAATTCCGGGCCCAAAAATGGCTAAAAATTCCCATGCCCGGGAGGCTGCCTTGCTTCCAA
  AGGGGGTTTTTGGGGCGGGACCCCCCCCCCTTTCCTTTTGAAAGGCCAAGGCCAAAACGGGATTTGGCCA
  ACCCCTATTTTCCTTTTAGGGGGCCCCCCCCCAAGGGTTGGCCCAAACCCCCCCCCCCCCCGGGGAACAC
  GAAACCCCAATTCTTGAACCCCTGGACCCCTTTTTTACCAAGCCCCTCCCCCAAAAAGGGCCAAATTTTT
  AAAAACCTACAGGGGGGCCCCCCCCCCCCTTGCTTTTTTTTTTCCCCTGAGTGCCCCGTGTGTCCCAAAA
  AAAAATTCTTTAACACCTCTTTGTACGAAAACATTTTTTAGGGGCGCTCCGACAACGCCTATTTTTCCCC
  CCGGGGAAGCATGAGTATTTTTATAAAAAAACACCTATGGAGCGCAGACCACCGAGCGTTCTCTCTTAAG
  TAGTGATTCTTCGTGTCTACTTCGTGTAGAGCACCCACCCCGCCGTGTAGATGCGCGCGCATGTAGACTT
  GCTT
  
  Kinase domain sequence

  >SC323680 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CTGGAGCTGCTACTASGCTTAGCTGCTCAKSAKMSATGGGMRGKCACASGGCCTGCTGAGGGACGGGACG
  GAGGTGGCCGTGATGATCCAgTACCCCGGCATAGCCCAGAGCATTCAGAGCGATGTCCAGAACCTGCTGG
  CAGTACTCAAGATGAGCGCGGCCCTGCCCGCGGGCCTGTTTGCCGAGCAGAGCCTGCAGGCCTTGCASCA
  GGAGCTGGCTTGGGAGTGTGACTACCGTCRTGAGGCGGCTTGTGC
  
• Restriction Sites: Please inquire
• ACCN: NM_024876
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024876.1, NP_079152.1
• RefSeq Size: 1949 bp
• RefSeq ORF: 501 bp
• Locus ID: 79934
• UniProt ID: Q96D53
• Cytogenetics: 19q13.2
• Domains: ABC1
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a protein with two copies of a domain found in protein kinases. The encoded protein has a complete protein kinase catalytic domain, and a truncated domain that contains only the active and binding sites of the protein kinase domain, however, it is not known whether the protein has any kinase activity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).