CLK2 (NM_003993) Human Untagged Clone

CAT#: SC323449

CLK2 (untagged)-Kinase deficient mutant (K192M) of Human CDC-like kinase 2 (CLK2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLK2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323449 sequence for NM_003993 edited (data generated by NextGen Sequencing)
  ATGCCGCATCCTCGAAGGTACCACTCCTCAGAGCGAGGCAGCCGGGGGAGTTACCGTGAA
  CACTATCGGAGCCGAAAGCATAAGCGACGAAGAAGTCGCTCCTGGTCAAGTAGTAGTGAC
  CGGACACGACGGCGTCGGCGAGAGGACAGCTACCATGTCCGTTCTCGAAGCAGTTATGAT
  GATCGTTCGTCCGACCGGAGGGTGTATGACCGGCGATACTGTGGCAGCTACAGACGCAAC
  GATTATAGCCGGGATCGGGGAGATGCCTACTATGACACAGACTATCGGCATTCCTATGAA
  TATCAGCGGGAGAACAGCAGTTACCGCAGCCAGCGCAGCAGCCGGAGGAAGCACAGACGG
  CGGAGGAGGCGCAGCCGGACATTTAGCCGCTCATCTTCGCACAGCAGCCGGAGAGCCAAG
  AGTGTAGAGGACGACGCTGAGGGCCACCTCATCTACCACGTCGGGGACTGGCTACAAGAG
  CGATATGAAATCGTTAGCACCTTAGGAGAGGGGACCTTCGGCCGAGTTGTACAATGTGTT
  GACCATCGCAGGGGTGGGGCTCGAGTTGCCCTGATGATCATTAAGAATGTGGAGAAGTAC
  AAGGAAGCAGCTCGACTTGAGATCAACGTGCTAGAGAAAATCAATGAGAAAGACCCTGAC
  AACAAGAACCTCTGTGTCCAGATGTTTGACTGGTTTGACTACCATGGCCACATGTGTATC
  TCCTTTGAGCTTCTGGGCCTTAGCACCTTCGATTTCCTCAAAGACAACAACTACCTGCCC
  TACCCCATCCACCAAGTGCGCCACATGGCCTTCCAGCTGTGCCAGGCTGTCAAGTTCCTC
  CATGATAACAAGCTGACACATACAGACCTCAAGCCTGAAAATATTCTGTTTGTGAATTCA
  GACTATGAGCTCACCTACAACCTAGAGAAGAAGCGAGATGAGCGCAGTGTGAAGAGCACA
  GCTGTGCGGGTGGTAGACTTTGGCAGTGCCACCTTTGACCATGAGCACCATAGCACCATT
  GTCTCCACTCGCCATTACCGAGCACCAGAAGTCATCCTTGAGTTGGGCTGGTCACAGCCT
  TGTGATGTGTGGAGTATAGGCTGCATCATCTTTGAATACTATGTGGGATTCACCCTCTTC
  CAGACCCATGACAACAGAGAGCATCTAGCCATGATGGAAAGGATCTTGGGTCCTATCCCT
  TCCCGGATGATCCGAAAGACAAGAAAGCAGAAATATTTTTACCGGGGTCGCCTGGATTGG
  GATGAGAACACATCAGCTGGGCGCTATGTTCGTGAGAACTGCAAACCGCTGCGGCGGTAT
  CTGACCTCAGAGGCAGAGGAACACCACCAGCTCTTCGATCTGATTGAAAGCATGCTAGAG
  TATGAACCAGCTAAGCGGCTGACCTTGGGTGAAGCCCTTCAGCATCCTTTCTTCGCCCGC
  CTTCGGGCTGAGCCGCCCAACAAGTTGTGGGACTCCAGTCGGGATATCAGTCGGTGA
  
  Clone variation with respect to NM_003993.2
  575 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_003993 unedited
  CCCGCCCGCAACAGCAAAGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGT
  GAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGGAGAGGCCAGA
  GCCGGAGACCGAGCTGGGATCGGGCCCCGGGCGGGGGCGGTGCGAGCGGCGCCAAGCAGATCTTAGGGGC
  GGGGACGGAGCCGGGGCGGGCGGGACTGAAGCGGAGCCCGGGAACGGGGCGGGAGGTCCCAGGGTCCCCG
  GGTTGGGGGGGGTGGAGCAGGTTTAGGAATAGCAGACAGCAAAACCTTACAAGCCCAACGGATGAATTAT
  AGACGAAAAAAAAACATAGCAAAAAATCCCAGGCAAAAAAATAAACAAAAAACAAGGGGGAGA
  
  Kinase domain sequence

  >SC323449 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TTYGAGKKGTTRRACTTAGAGAGGGGACTTCGGCCGAGTTGTACAATGTGTTGACCATCGCAGCCTCTCC
  YSYCAACTTACAGGGGTGGGGCTCGAGTTGCCCTGATGATCATTAAGAATGTGGAGAAGTACAAGGAAGC
  AGCTCGACTTGAGATCAACGTGCTAGAGAAAATCAATGAGAAAGACCCTGACAACAAGAACCTCTGTGTC
  CAGATGTTTGACTGGTTTGACTACCATGGCCACATGTGTATCTCC
  
• Restriction Sites: Please inquire
• ACCN: NM_003993
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003993.2, NP_003984.2
• RefSeq Size: 2175 bp
• RefSeq ORF: 1497 bp
• Locus ID: 1196
• UniProt ID: P49760
• Cytogenetics: 1q22
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a dual specificity protein kinase that phosphorylates serine/threonine and tyrosine-containing substrates. Activity of this protein regulates serine- and arginine-rich (SR) proteins of the spliceosomal complex, thereby influencing alternative transcript splicing. Chromosomal translocations have been characterized between this locus and the PAFAH1B3 (platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa)) gene on chromosome 19, resulting in the production of a fusion protein. Note that this gene is distinct from the TELO2 gene (GeneID:9894), which shares the CLK2 alias, but encodes a protein that is involved in telomere length regulation. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.