Eph receptor B1 (EPHB1) (NM_004441) Human Untagged Clone

CAT#: SC323437

EPHB1 (untagged)-Kinase deficient mutant (K651M) of Human EPH receptor B1 (EPHB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Eph receptor B1
• Synonyms: ELK; EPHT2; Hek6; NET
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323437 sequence for NM_004441 edited (data generated by NextGen Sequencing)
  ATGGCCCTGGATTATCTACTACTGCTCCTCCTGGCATCCGCAGTGGCTGCGATGGAAGAA
  ACGTTAATGGACACCAGAACGGCTACTGCAGAGCTGGGCTGGACGGCCAATCCTGCGTCC
  GGGTGGGAAGAAGTCAGTGGCTACGATGAAAACCTGAACACCATCCGCACCTACCAGGTG
  TGCAATGTCTTCGAGCCCAACCAGAACAATTGGCTGCTCACCACCTTCATCAACCGGCGG
  GGGGCCCATCGCATCTACACAGAGATGCGCTTCACTGTGAGAGACTGCAGCAGCCTCCCT
  AATGTCCCAGGATCCTGCAAGGAGACCTTCAACTTGTATTACTATGAGACTGACTCTGTC
  ATTGCCACCAAGAAGTCAGCCTTCTGGTCTGAGGCCCCCTACCTCAAAGTAGACACCATT
  GCTGCAGATGAGAGTTTCTCCCAGGTGGACTTTGGGGGAAGGCTGATGAAGGTAAACACA
  GAAGTCAGGAGCTTTGGGCCTCTTACTCGGAATGGTTTTTACCTCGCTTTTCAGGATTAT
  GGAGCCTGTATGTCTCTTCTTTCTGTCCGTGTCTTCTTCAAAAAGTGTCCCAGCATTGTG
  CAAAATTTTGCAGTGTTTCCAGAGACTATGACAGGGGCAGAGAGCACATCTCTGGTGATT
  GCTCGGGGCACATGCATCCCCAACGCAGAGGAAGTGGACGTGCCCATCAAACTCTACTGC
  AACGGGGATGGGGAATGGATGGTGCCTATTGGGCGATGCACCTGCAAGCCTGGCTATGAG
  CCTGAGAACAGCGTGGCATGCAAGGCTTGCCCTGCAGGGACATTCAAGGCCAGCCAGGAA
  GCTGAAGGCTGCTCCCACTGCCCCTCCAACAGCCGCTCCCCTGCAGAGGCGTCTCCCATC
  TGCACCTGTCGGACCGGTTATTACCGAGCGGACTTTGACCCTCCAGAAGTGGCATGCACT
  AGCGTCCCATCAGGTCCCCGCAATGTTATCTCCATCGTCAATGAGACGTCCATCATTCTG
  GAGTGGCACCCTCCAAGGGAGACAGGTGGGCGGGATGATGTGACCTACAACATCATCTGC
  AAAAAGTGCCGGGCAGACCGCCGGAGCTGCTCCCGCTGTGACGACAATGTGGAGTTTGTG
  CCCAGGCAGCTGGGCCTGACGGAGTGCCGCGTCTCCATCAGCAGCCTGTGGGCCCACACC
  CCCTACACCTTTGACATCCAGGCCATCAATGGAGTCTCCAGCAAGAGTCCCTTCCCCCCA
  CAGCACGTCTCTGTCAACATCACCACAAACCAAGCCGCCCCCTCCACCGTTCCCATCATG
  CACCAAGTCAGTGCCACTATGAGGAGCATCACCTTGTCATGGCCACAGCCGGAGCAGCCC
  AATGGCATCATCCTGGACTATGAGATCCGGTACTATGAGAAGGAACACAATGAGTTCAAC
  TCCTCCATGGCCAGGAGTCAGACCAACACAGCAAGGATTGATGGGCTGCGGCCTGGCATG
  GTATATGTGGTACAGGTGCGTGCCCGCACTGTTGCTGGCTACGGCAAGTTCAGTGGCAAG
  ATGTGCTTCCAGACTCTGACTGACGATGATTACAAGTCAGAGCTGAGGGAGCAGCTGCCC
  CTGATTGCTGGCTCGGCAGCGGCCGGGGTCGTGTTCGTTGTGTCCTTGGTGGCCATCTCT
  ATCGTCTGTAGCAGGAAACGGGCTTATAGCAAAGAGGCTGTGTACAGCGATAAGCTCCAG
  CATTACAGCACAGGCCGAGGCTCCCCAGGGATGAAGATCTACATTGACCCCTTCACTTAC
  GAGGATCCCAACGAAGCTGTCCGGGAGTTTGCCAAGGAGATTGATGTATCTTTTGTGAAA
  ATTGAAGAGGTCATCGGAGCAGGGGAGTTTGGAGAAGTGTACAAGGGGCGTTTGAAACTG
  CCAGGCAAGAGGGAAATCTACGTGGCCATCATGACCCTGAAGGCAGGGTACTCGGAGAAG
  CAGCGTCGGGACTTTCTGAGTGAGGCGAGCATCATGGGCCAGTTCGACCATCCTAACATC
  ATTCGCCTGGAGGGTGTGGTCACCAAGAGTCGGCCTGTCATGATCATCACAGAGTTCATG
  GAGAATGGTGCATTGGATTCTTTCCTCAGGCAAAATGACGGGCAGTTCACCGTGATCCAG
  CTTGTGGGTATGCTCAGGGGCATCGCTGCTGGCATGAAGTACCTGGCTGAGATGAATTAT
  GTGCATCGGGACCTGGCTGCTAGGAACATTCTGGTCAACAGTAACCTGGTGTGCAAGGTG
  TCCGACTTTGGCCTCTCCCGCTACCTCCAGGATGACACCTCAGATCCCACCTACACCAGC
  TCCTTGGGAGGGAAGATCCCTGTGAGATGGACAGCTCCAGAGGCCATCGCCTACCGCAAG
  TTCACTTCAGCCAGCGACGTTTGGAGCTATGGGATCGTCATGTGGGAAGTCATGTCATTT
  GGAGAGAGACCCTATTGGGATATGTCCAACCAAGATGTCATCAATGCCATCGAGCAGGAC
  TACCGGCTGCCCCCACCCATGGACTGTCCAGCTGCTCTACACCAGCTCATGCTGGACTGT
  TGGCAGAAGGACCGGAACAGCCGGCCCCGGTTTGCGGAGATTGTCAACACCCTAGATAAG
  ATGATCCGGAACCCGGCAAGTCTCAAGACTGTGGCAACCATCACCGCCGTGCCTTCCCAG
  CCCCTGCTCGACCGCTCCATCCCAGACTTCACGGCCTTTACCACCGTGGATGACTGGCTC
  AGCGCCATCAAAATGGTCCAGTACAGGGACAGCTTCCTCACTGCTGGCTTCACCTCCCTC
  CAGCTGGTCACCCAGATGACATCAGAAGACCTCCTGAGAATAGGCATCACCTTGGCAGGC
  CATCAGAAGAAGATCCTGAACAGCATTCATTCTATGAGGGTCCAGATAAGTCAGTCACCA
  ACGGCAATGGCATGA
  
  Clone variation with respect to NM_004441.4
  435 c=>t;1952 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004441 unedited
  ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCACACCCACGC
  GCGCCCGCACCGCCCCACGCGCACACACTCCTGCCCACGCCCACGCAGCGCTCCGGGAAGTCCGGTCCGG
  GCGAGAGCGCGAAAGGATACCGAGAAGCCACCCGCGGAGAGCGCAGCGGCGCCCTGGGACGCGGCGCTCT
  CCCGGCGCTGCTGCCTCGGCTTGGTCTCGGGCCTGCGGGGCCGTCGGCCCGGCGATGGCCCTGGATAATC
  TACTACTGCTCCTCCCTGGCATCCGCATTGGCCTGCGAAGGGAAGAAAACGTTAATGGGACCCCAAACCG
  GCTACTGGCAAAGCTGGGCTGGGACGGCCATTCCGGCTTCCGGTGGGAAAAAGGTAGTGGCCTCGAAGAA
  AAACCGAAACCCCTCCCGCCCCTCCCGGGGGTGCAATGTTTTCGAGCCCCCCCAGAACATTGGCTGGCTC
  CCACCCTTACTAACCGGGGGGGGGCCCATCGAATCTACACAGAAATGCCCTTCTGGGAGAGAATGCAGCA
  CCCCTCCTTTTGTCTCCCATATCCGGAGGGGACCCCTACACTTTATATACTTTAAACGTGACGTGTATGT
  GCCACAGAGACTCCCCCTGTGTGAGAGCCTCATCTAAAGACACACTGTGCGAGAGAGATTTCTCATGGGT
  TTGGGAAGCGGGGAAGGGTACAAGCTGGGTTGGCGCTATCGAAGGTGTACACCTTTGAATGAGCGCGTGT
  GCTCTTTGCCGGTTTCAATGCACATGGACATGATGTTCGACTACAGAGCAGCATCATCGAAGTGCGAGCA
  CTCACCAGAGAGTGCTGACAA
  
  Kinase domain sequence

  >SC323437 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CRAGGTYMTCGGAGCAGGGAGTTTGGAGAAGTGTACAAGGGGCGTTTGAAACTGCCAGGCAMSASGGAAA
  TCTACGTGGCCATCATGACCCTGAAGGCAGGGTACTCGGAGAAGCAGCGTCGGGACTTTCTGAGTGAGGC
  GAGCATCATGGGCCAGTTCGACCATCCTAACATCATTCGCCTGGAGGGTGTGGTCACCAAGAGTCGGCCT
  GTCATGATCATCACAGAGTTCATGGAGAATGGTGCATTGGATTCT
  
• Restriction Sites: Please inquire
• ACCN: NM_004441
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004441.3, NP_004432.1
• RefSeq Size: 4691 bp
• RefSeq ORF: 2955 bp
• Locus ID: 2047
• UniProt ID: P54762
• Cytogenetics: 3q22.2
• Domains: pkinase, EPH_lbd, TyrKc, SAM, S_TKc, FN3
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Axon guidance
• Gene Summary: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008]