Eph receptor B1 (EPHB1) (NM_004441) Human Tagged ORF Clone

CAT#: RC214301

EPHB1 (Myc-DDK-tagged)-Human EPH receptor B1 (EPHB1)

Product Images

Circular map for RC214301

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eph receptor B1
• Synonyms: ELK; EPHT2; Hek6; NET
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214301 representing NM_004441
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGGATTATCTACTACTGCTCCTCCTGGCATCCGCAGTGGCTGCGATGGAAGAAACGTTAATGG
  ACACCAGAACGGCTACTGCAGAGCTGGGCTGGACGGCCAATCCTGCGTCCGGGTGGGAAGAAGTCAGTGG
  CTACGATGAAAACCTGAACACCATCCGCACCTACCAGGTGTGCAATGTCTTCGAGCCCAACCAGAACAAT
  TGGCTGCTCACCACCTTCATCAACCGGCGGGGGGCCCATCGCATCTACACAGAGATGCGCTTCACTGTGA
  GAGACTGCAGCAGCCTCCCTAATGTCCCAGGATCCTGCAAGGAGACCTTCAACTTGTATTACTATGAGAC
  TGACTCTGTCATTGCCACCAAGAAGTCAGCCTTCTGGTCTGAGGCCCCCTACCTCAAAGTAGACACCATT
  GCTGCAGATGAGAGCTTCTCCCAGGTGGACTTTGGGGGAAGGCTGATGAAGGTAAACACAGAAGTCAGGA
  GCTTTGGGCCTCTTACTCGGAATGGTTTTTACCTCGCTTTTCAGGATTATGGAGCCTGTATGTCTCTTCT
  TTCTGTCCGTGTCTTCTTCAAAAAGTGTCCCAGCATTGTGCAAAATTTTGCAGTGTTTCCAGAGACTATG
  ACAGGGGCAGAGAGCACATCTCTGGTGATTGCTCGGGGCACATGCATCCCCAACGCAGAGGAAGTGGACG
  TGCCCATCAAACTCTACTGCAACGGGGATGGGGAATGGATGGTGCCTATTGGGCGATGCACCTGCAAGCC
  TGGCTATGAGCCTGAGAACAGCGTGGCATGCAAGGCTTGCCCTGCAGGGACATTCAAGGCCAGCCAGGAA
  GCTGAAGGCTGCTCCCACTGCCCCTCCAACAGCCGCTCCCCTGCAGAGGCGTCTCCCATCTGCACCTGTC
  GGACCGGTTATTACCGAGCGGACTTTGACCCTCCAGAAGTGGCATGCACTAGCGTCCCATCAGGTCCCCG
  CAATGTTATCTCCATCGTCAATGAGACGTCCATCATTCTGGAGTGGCACCCTCCAAGGGAGACAGGTGGG
  CGGGATGATGTGACCTACAACATCATCTGCAAAAAGTGCCGGGCAGACCGCCGGAGCTGCTCCCGCTGTG
  ACGACAATGTGGAGTTTGTGCCCAGGCAGCTGGGCCTGACGGAGTGCCGCGTCTCCATCAGCAGCCTGTG
  GGCCCACACCCCCTACACCTTTGACATCCAGGCCATCAATGGAGTCTCCAGCAAGAGTCCCTTCCCCCCA
  CAGCACGTCTCTGTCAACATCACCACAAACCAAGCCGCCCCCTCCACCGTTCCCATCATGCACCAAGTCA
  GTGCCACTATGAGGAGCATCACCTTGTCATGGCCACAGCCGGAGCAGCCCAATGGCATCATCCTGGACTA
  TGAGATCCGGTACTATGAGAAGGAACACAATGAGTTCAACTCCTCCATGGCCAGGAGTCAGACCAACACA
  GCAAGGATTGATGGGCTGCGGCCTGGCATGGTATATGTGGTACAGGTGCGTGCCCGCACTGTTGCTGGCT
  ACGGCAAGTTCAGTGGCAAGATGTGCTTCCAGACTCTGACTGACGATGATTACAAGTCAGAGCTGAGGGA
  GCAGCTGCCCCTGATTGCTGGCTCGGCAGCGGCCGGGGTCGTGTTCGTTGTGTCCTTGGTGGCCATCTCT
  ATCGTCTGTAGCAGGAAACGGGCTTATAGCAAAGAGGCTGTGTACAGCGATAAGCTCCAGCATTACAGCA
  CAGGCCGAGGCTCCCCAGGGATGAAGATCTACATTGACCCCTTCACTTATGAGGATCCCAACGAAGCTGT
  CCGGGAGTTTGCCAAGGAGATTGATGTATCTTTTGTGAAAATTGAAGAGGTCATCGGAGCAGGGGAGTTT
  GGAGAAGTGTACAAGGGGCGTTTGAAACTGCCAGGCAAGAGGGAAATCTACGTGGCCATCAAGACCCTGA
  AGGCAGGGTACTCGGAGAAGCAGCGTCGGGACTTTCTGAGTGAGGCGAGCATCATGGGCCAGTTCGACCA
  TCCTAACATCATTCGCCTGGAGGGTGTGGTCACCAAGAGTCGGCCTGTCATGATCATCACAGAGTTCATG
  GAGAATGGTGCATTGGATTCTTTCCTCAGGCAAAATGACGGGCAGTTCACCGTGATCCAGCTTGTGGGTA
  TGCTCAGGGGCATCGCTGCTGGCATGAAGTACCTGGCTGAGATGAATTATGTGCATCGGGACCTGGCTGC
  TAGGAACATTCTGCTCAACAGTAACCTGGTGTGCAAGGTGTCCGACTTTGGCCTCTCCCGCTACCTCCAG
  GATGACACCTCAGATCCCACCTACACCAGCTCCTTGGGAGGGAAGATCCCTGTGAGATGGACAGCTCCAG
  AGGCCATCGCCTACCGCAAGTTCACTTCAGCCAGCGACGTTTGGAGCTATGGGATCGTCATGTGGGAAGT
  CATGTCATTTGGAGAGAGACCCTATTGGGATATGTCCAACCAAGATGTCATCAATGCCATCGAGCAGGAC
  TACCGGCTGCCCCCACCCATGGACTGTCCAGCTGCTCTACACCAGCTCATGCTGGACTGTTGGCAGAAGG
  ACCGGAACAGCCGGCCCCGGTTTGCGGAGATTGTCAACACCCTAGATAAGATGATCCGGAACCCGGCAAG
  TCTCAAGACTGTGGCAACCATCACCGCCGTGCCTTCCCAGCCCCTGCTCGACCGCTCCATCCCAGACTTC
  ACGGCCTTTACCACCGTGGATGACTGGCTCAGCGCCATCAAAATGGTCCAGTACAGGGACAGCTTCCTCA
  CTGCTGGCTTCACCTCCCTCCAGCTGGTCACCCAGATGACATCAGAAGACCTCCTGAGAATAGGCATCAC
  CTTGGCAGGCCATCAGAAGAAGATCCTGAACAGCATTCATTCTATGAGGGTCCAGATAAGTCAGTCACCA
  ACGGCAATGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214301 representing NM_004441
  Red=Cloning site Green=Tags(s)
  
  MALDYLLLLLLASAVAAMEETLMDTRTATAELGWTANPASGWEEVSGYDENLNTIRTYQVCNVFEPNQNN
  WLLTTFINRRGAHRIYTEMRFTVRDCSSLPNVPGSCKETFNLYYYETDSVIATKKSAFWSEAPYLKVDTI
  AADESFSQVDFGGRLMKVNTEVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETM
  TGAESTSLVIARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGTFKASQE
  AEGCSHCPSNSRSPAEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVNETSIILEWHPPRETGG
  RDDVTYNIICKKCRADRRSCSRCDDNVEFVPRQLGLTECRVSISSLWAHTPYTFDIQAINGVSSKSPFPP
  QHVSVNITTNQAAPSTVPIMHQVSATMRSITLSWPQPEQPNGIILDYEIRYYEKEHNEFNSSMARSQTNT
  ARIDGLRPGMVYVVQVRARTVAGYGKFSGKMCFQTLTDDDYKSELREQLPLIAGSAAAGVVFVVSLVAIS
  IVCSRKRAYSKEAVYSDKLQHYSTGRGSPGMKIYIDPFTYEDPNEAVREFAKEIDVSFVKIEEVIGAGEF
  GEVYKGRLKLPGKREIYVAIKTLKAGYSEKQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMIITEFM
  ENGALDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLAEMNYVHRDLAARNILLNSNLVCKVSDFGLSRYLQ
  DDTSDPTYTSSLGGKIPVRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQD
  YRLPPPMDCPAALHQLMLDCWQKDRNSRPRFAEIVNTLDKMIRNPASLKTVATITAVPSQPLLDRSIPDF
  TAFTTVDDWLSAIKMVQYRDSFLTAGFTSLQLVTQMTSEDLLRIGITLAGHQKKILNSIHSMRVQISQSP
  TAMA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004441
• ORF Size: 2952 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004441.5
• RefSeq Size: 4536 bp
• RefSeq ORF: 2955 bp
• Locus ID: 2047
• UniProt ID: P54762
• Cytogenetics: 3q22.2
• Domains: pkinase, EPH_lbd, TyrKc, SAM, S_TKc, FN3
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Axon guidance
• MW: 109.9 kDa
• Gene Summary: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008]