Eph receptor B1(EPHB1) (NM_004441) Human Tagged ORF Clone

CAT#: RC214301

EPHB1 (Myc-DDK-tagged)-Human EPH receptor B1 (EPHB1)

Clone Modification / Custom Cloning Service

  • TrueORF®

USD 629.00


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Size
    • 10 ug

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Specifications

Product Data
Product Name Eph receptor B1(EPHB1) (NM_004441) Human Tagged ORF Clone
Symbol EPHB1
Synonyms ELK; EPHT2; Hek6; NET
Vector pCMV6-Entry
Sequence Data
ORF Nucleotide Sequence
>RC214301 representing NM_004441
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTGGATTATCTACTACTGCTCCTCCTGGCATCCGCAGTGGCTGCGATGGAAGAAACGTTAATGG
ACACCAGAACGGCTACTGCAGAGCTGGGCTGGACGGCCAATCCTGCGTCCGGGTGGGAAGAAGTCAGTGG
CTACGATGAAAACCTGAACACCATCCGCACCTACCAGGTGTGCAATGTCTTCGAGCCCAACCAGAACAAT
TGGCTGCTCACCACCTTCATCAACCGGCGGGGGGCCCATCGCATCTACACAGAGATGCGCTTCACTGTGA
GAGACTGCAGCAGCCTCCCTAATGTCCCAGGATCCTGCAAGGAGACCTTCAACTTGTATTACTATGAGAC
TGACTCTGTCATTGCCACCAAGAAGTCAGCCTTCTGGTCTGAGGCCCCCTACCTCAAAGTAGACACCATT
GCTGCAGATGAGAGCTTCTCCCAGGTGGACTTTGGGGGAAGGCTGATGAAGGTAAACACAGAAGTCAGGA
GCTTTGGGCCTCTTACTCGGAATGGTTTTTACCTCGCTTTTCAGGATTATGGAGCCTGTATGTCTCTTCT
TTCTGTCCGTGTCTTCTTCAAAAAGTGTCCCAGCATTGTGCAAAATTTTGCAGTGTTTCCAGAGACTATG
ACAGGGGCAGAGAGCACATCTCTGGTGATTGCTCGGGGCACATGCATCCCCAACGCAGAGGAAGTGGACG
TGCCCATCAAACTCTACTGCAACGGGGATGGGGAATGGATGGTGCCTATTGGGCGATGCACCTGCAAGCC
TGGCTATGAGCCTGAGAACAGCGTGGCATGCAAGGCTTGCCCTGCAGGGACATTCAAGGCCAGCCAGGAA
GCTGAAGGCTGCTCCCACTGCCCCTCCAACAGCCGCTCCCCTGCAGAGGCGTCTCCCATCTGCACCTGTC
GGACCGGTTATTACCGAGCGGACTTTGACCCTCCAGAAGTGGCATGCACTAGCGTCCCATCAGGTCCCCG
CAATGTTATCTCCATCGTCAATGAGACGTCCATCATTCTGGAGTGGCACCCTCCAAGGGAGACAGGTGGG
CGGGATGATGTGACCTACAACATCATCTGCAAAAAGTGCCGGGCAGACCGCCGGAGCTGCTCCCGCTGTG
ACGACAATGTGGAGTTTGTGCCCAGGCAGCTGGGCCTGACGGAGTGCCGCGTCTCCATCAGCAGCCTGTG
GGCCCACACCCCCTACACCTTTGACATCCAGGCCATCAATGGAGTCTCCAGCAAGAGTCCCTTCCCCCCA
CAGCACGTCTCTGTCAACATCACCACAAACCAAGCCGCCCCCTCCACCGTTCCCATCATGCACCAAGTCA
GTGCCACTATGAGGAGCATCACCTTGTCATGGCCACAGCCGGAGCAGCCCAATGGCATCATCCTGGACTA
TGAGATCCGGTACTATGAGAAGGAACACAATGAGTTCAACTCCTCCATGGCCAGGAGTCAGACCAACACA
GCAAGGATTGATGGGCTGCGGCCTGGCATGGTATATGTGGTACAGGTGCGTGCCCGCACTGTTGCTGGCT
ACGGCAAGTTCAGTGGCAAGATGTGCTTCCAGACTCTGACTGACGATGATTACAAGTCAGAGCTGAGGGA
GCAGCTGCCCCTGATTGCTGGCTCGGCAGCGGCCGGGGTCGTGTTCGTTGTGTCCTTGGTGGCCATCTCT
ATCGTCTGTAGCAGGAAACGGGCTTATAGCAAAGAGGCTGTGTACAGCGATAAGCTCCAGCATTACAGCA
CAGGCCGAGGCTCCCCAGGGATGAAGATCTACATTGACCCCTTCACTTATGAGGATCCCAACGAAGCTGT
CCGGGAGTTTGCCAAGGAGATTGATGTATCTTTTGTGAAAATTGAAGAGGTCATCGGAGCAGGGGAGTTT
GGAGAAGTGTACAAGGGGCGTTTGAAACTGCCAGGCAAGAGGGAAATCTACGTGGCCATCAAGACCCTGA
AGGCAGGGTACTCGGAGAAGCAGCGTCGGGACTTTCTGAGTGAGGCGAGCATCATGGGCCAGTTCGACCA
TCCTAACATCATTCGCCTGGAGGGTGTGGTCACCAAGAGTCGGCCTGTCATGATCATCACAGAGTTCATG
GAGAATGGTGCATTGGATTCTTTCCTCAGGCAAAATGACGGGCAGTTCACCGTGATCCAGCTTGTGGGTA
TGCTCAGGGGCATCGCTGCTGGCATGAAGTACCTGGCTGAGATGAATTATGTGCATCGGGACCTGGCTGC
TAGGAACATTCTGCTCAACAGTAACCTGGTGTGCAAGGTGTCCGACTTTGGCCTCTCCCGCTACCTCCAG
GATGACACCTCAGATCCCACCTACACCAGCTCCTTGGGAGGGAAGATCCCTGTGAGATGGACAGCTCCAG
AGGCCATCGCCTACCGCAAGTTCACTTCAGCCAGCGACGTTTGGAGCTATGGGATCGTCATGTGGGAAGT
CATGTCATTTGGAGAGAGACCCTATTGGGATATGTCCAACCAAGATGTCATCAATGCCATCGAGCAGGAC
TACCGGCTGCCCCCACCCATGGACTGTCCAGCTGCTCTACACCAGCTCATGCTGGACTGTTGGCAGAAGG
ACCGGAACAGCCGGCCCCGGTTTGCGGAGATTGTCAACACCCTAGATAAGATGATCCGGAACCCGGCAAG
TCTCAAGACTGTGGCAACCATCACCGCCGTGCCTTCCCAGCCCCTGCTCGACCGCTCCATCCCAGACTTC
ACGGCCTTTACCACCGTGGATGACTGGCTCAGCGCCATCAAAATGGTCCAGTACAGGGACAGCTTCCTCA
CTGCTGGCTTCACCTCCCTCCAGCTGGTCACCCAGATGACATCAGAAGACCTCCTGAGAATAGGCATCAC
CTTGGCAGGCCATCAGAAGAAGATCCTGAACAGCATTCATTCTATGAGGGTCCAGATAAGTCAGTCACCA
ACGGCAATGGCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC214301 representing NM_004441
Red=Cloning site Green=Tags(s)

MALDYLLLLLLASAVAAMEETLMDTRTATAELGWTANPASGWEEVSGYDENLNTIRTYQVCNVFEPNQNN
WLLTTFINRRGAHRIYTEMRFTVRDCSSLPNVPGSCKETFNLYYYETDSVIATKKSAFWSEAPYLKVDTI
AADESFSQVDFGGRLMKVNTEVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETM
TGAESTSLVIARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGTFKASQE
AEGCSHCPSNSRSPAEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVNETSIILEWHPPRETGG
RDDVTYNIICKKCRADRRSCSRCDDNVEFVPRQLGLTECRVSISSLWAHTPYTFDIQAINGVSSKSPFPP
QHVSVNITTNQAAPSTVPIMHQVSATMRSITLSWPQPEQPNGIILDYEIRYYEKEHNEFNSSMARSQTNT
ARIDGLRPGMVYVVQVRARTVAGYGKFSGKMCFQTLTDDDYKSELREQLPLIAGSAAAGVVFVVSLVAIS
IVCSRKRAYSKEAVYSDKLQHYSTGRGSPGMKIYIDPFTYEDPNEAVREFAKEIDVSFVKIEEVIGAGEF
GEVYKGRLKLPGKREIYVAIKTLKAGYSEKQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMIITEFM
ENGALDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLAEMNYVHRDLAARNILLNSNLVCKVSDFGLSRYLQ
DDTSDPTYTSSLGGKIPVRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQD
YRLPPPMDCPAALHQLMLDCWQKDRNSRPRFAEIVNTLDKMIRNPASLKTVATITAVPSQPLLDRSIPDF
TAFTTVDDWLSAIKMVQYRDSFLTAGFTSLQLVTQMTSEDLLRIGITLAGHQKKILNSIHSMRVQISQSP
TAMA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_004441
ORF Size 2952 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reference Data
RefSeq NM_004441.1, NM_004441.2, NM_004441.3, NM_004441.4, NP_004432
RefSeq Size 4536
RefSeq ORF 2955
Locus ID 2047
Cytogenetics 3q22.2
Domains pkinase, EPH_lbd, TyrKc, SAM, S_TKc, FN3
Protein Families Protein Kinase, Transmembrane, Druggable Genome
Protein Pathways Axon guidance
MW 109.9 kDa
Gene Summary Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008].
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Citations (1)