CLK4 (NM_020666) Human Untagged Clone

CAT#: SC323425

CLK4 (untagged)-Kinase deficient mutant (K189M) of Human CDC-like kinase 4 (CLK4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLK4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_020666, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGGCATTCCAAAAGAACTCACTGTCCTGATTGGGATAGCAGAGAAAGCTGGGGACATGAAAGCTATC
  GTGGAAGTCACAAGCGGAAGAGGAGATCTCATAGTAGCACACAAGAGAACAGGCATTGTAAACCACATCA
  CCAGTTTAAAGAATCTGATTGTCATTATTTAGAAGCAAGGTCCTTGAATGAGCGAGATTATCGGGACCGG
  AGATACGTTGACGAATACAGGAATGACTACTGTGAAGGATATGTTCCTAGACATTATCACAGAGACATTG
  AAAGCGGGTATCGAATCCACTGCAGTAAATCTTCAGTCCGCAGCAGGAGAAGCAGTCCTAAAAGGAAGCG
  CAATAGACACTGTTCAAGTCATCAGTCACGTTCGAAGAGCCACCGAAGGAAAAGATCCAGGAGTATAGAG
  GATGATGAGGAGGGTCACCTGATCTGTCAAAGTGGAGACGTTCTAAGAGCAAGATATGAAATCGTGGACA
  CTTTGGGTGAAGGAGCCTTTGGCAAAGTTGTAGAGTGCATTGATCATGGCATGGATGGCATGCATGTAGC
  AGTGAAAATCGTAAAAAATGTAGGCCGTTACCGTGAAGCAGCTCGTTCAGAAATCCAAGTATTAGAGCAC
  TTAAATAGTACTGATCCCAATAGTGTCTTCCGATGTGTCCAGATGCTAGAATGGTTTGATCATCATGGTC
  ATGTTTGTATTGTGTTTGAACTACTGGGACTTAGTACTTACGATTTCATTAAAGAAAACAGCTTTCTGCC
  ATTTCAAATTGACCACATCAGGCAGATGGCGTATCAGATCTGCCAGTCAATAAATTTTTTACATCATAAT
  AAATTAACCCATACAGATCTGAAGCCTGAAAATATTTTGTTTGTGAAGTCTGACTATGTAGTCAAATATA
  ATTCTAAAATGAAACGTGATGAACGCACACTGAAAAACACAGATATCAAAGTTGTTGACTTTGGAAGTGC
  AACGTATGATGATGAACATCACAGTACTTTGGTGTCTACCCGGCACTACAGAGCTCCCGAGGTCATTTTG
  GCTTTAGGTTGGTCTCAGCCTTGTGATGTTTGGAGCATAGGTTGCATTCTTATTGAATATTACCTTGGTT
  TCACAGTCTTTCAGACTCATGATAGTAAAGAGCACCTGGCAATGATGGAACGAATATTAGGACCCATACC
  ACAACACATGATTCAGAAAACAAGAAAACGCAAGTATTTTCACCATAACCAGCTAGATTGGGATGAACAC
  AGTTCTGCTGGTAGATATGTTAGGAGACGCTGCAAACCGTTGAAGGAATTTATGCTTTGTCATGATGAAG
  AACATGAGAAACTGTTTGACCTGGTTCGAAGAATGTTAGAATATGATCCAACTCAAAGAATTACCTTGGA
  TGAAGCATTGCAGCATCCTTTCTTTGACTTATTAAAAAAGAAATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_020666 unedited
  CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
  CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGGATTCTAGGGCG
  ACGGCGCTGCCGCCATTTTGTGGGGTGTTTGTCGCAGCGGCCGAGGAGGGAAGACGGCAGTTTGGCGACA
  TTTCTCGGCCGAAGGGCCATTTGCTTTTGCGGAGATGCGGCATTCCAAAAGAACTCACTGTCCTGATTGG
  GATAGCAGAGAAAGCTGGGGACATGAAAGCTATCGTGGAAGTCACAAGCGGAAGAGGAGATCTCATAGTA
  GCACACAAGAGAACAGGCATTGTAAACCACATCACCAGTTTTAAAGAATCTGATTGTCATTATTTAGAAG
  CAAGGTCCTTGAATGAGCGAGATTATCGGGACCGGAGATACGTTGACGATACAGGATGACTACTGTGAAG
  GATATGTTCCTAGACATTATCACAGAGACATTGAAAGCGGGTATCGAATCCACTGCAGTAATCTCAGTCC
  GCAGCAGGAGAGGCAGTCCTAAAGAGCGCATAGAACACCTTGTTCAGGTCATCAGTCACGTCGTGAGCCA
  CCGACGAAAAGATCCAGGAGTTATAGGATGATGAGGGAGGTTACCCTGGTATCCGGTCAATGGAAACGTT
  TCTAAAGAGCCAGATTGAATTCTGGGACCTCGGTGAGAGGACTTTGCCAACTGATGCCATGATCTGCTTG
  ATGCTAGCCGTCCACTGTGATACTCAAGTGGCCGTTACGGTGAACGCCGTGTCCAATTCCGCATTAGGCT
  TAGTGACGATTCCAATGTACCAGTCCAGTCC
  
  Kinase domain sequence

  >SC323425 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TGAMGTTCTAGAGCAGATATGAATCGTGGACACTTTGGGTGAAGGAGCCTTTGGCAAAGTTGTAGAGTGC
  ATTGATCATGGCATGGATGGCATGCATGTAGCAGTGATGATCGTAAAAAATGTAGGCCGTTACCGTGAAG
  CAGCTCGTTCAGAAATCCAAGTATTAGAGCACTTAAATAGTACTGATCCCAATAGTGTCTTCCGATGTGT
  CCAGATGCTAGAATGGTTTGATCATCATGGTCATGTTTGTATTGT
  
• Restriction Sites: Please inquire
• ACCN: NM_020666
• Insert Size: 2820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020666.2, NP_065717.1
• RefSeq Size: 2524 bp
• RefSeq ORF: 1446 bp
• Locus ID: 57396
• UniProt ID: Q9HAZ1
• Cytogenetics: 5q35.3
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The protein encoded by this gene belongs to the CDC2-like protein kinase (CLK) family. This protein kinase can interact with and phosphorylate the serine- and arginine-rich (SR) proteins, which are known to play an important role in the formation of spliceosomes, and thus may be involved in the regulation of alternative splicing. Studies in the Israeli sand rat Psammomys obesus suggested that the ubiquitin-like 5 (UBL5/BEACON), a highly conserved ubiquitin-like protein, may interact with and regulate the activity of this kinase. Multiple alternatively spliced transcript variants have been observed, but the full-length natures of which have not yet been determined. [provided by RefSeq, Jul 2008]