Casein Kinase 1 delta (CSNK1D) (NM_139062) Human Untagged Clone

CAT#: SC323403

CSNK1D (untagged)-Kinase deficient mutant (K38M) of Human casein kinase 1, delta (CSNK1D), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Casein Kinase 1 delta
• Synonyms: ASPS; CKI-delta; CKId; CKIdelta; FASPS2; HCKID
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323403 sequence for NM_139062 edited (data generated by NextGen Sequencing)
  ATGGAGCTGAGAGTCGGGAACAGGTACCGGCTGGGCCGGAAGATCGGCAGCGGCTCCTTC
  GGAGACATCTATCTCGGTACGGACATTGCTGCAGGAGAAGAGGTTGCCATCATGCTTGAA
  TGTGTCAAAACCAAACACCCTCAGCTCCACATTGAGAGCAAAATCTACAAGATGATGCAG
  GGAGGAGTGGGCATCCCCACCATCAGATGGTGCGGGGCAGAGGGGGACTACAACGTCATG
  GTGATGGAGCTGCTGGGGCCAAGCCTGGAGGACCTCTTCAACTTCTGCTCCAGGAAATTC
  AGCCTCAAAACCGTCCTGCTGCTTGCTGACCAAATGATCAGTCGCATCGAATACATTCAT
  TCAAAGAACTTCATCCACCGGGATGTGAAGCCAGACAACTTCCTCATGGGCCTGGGGAAG
  AAGGGCAACCTGGTGTACATCATCGACTTCGGGCTGGCCAAGAAGTACCGGGATGCACGC
  ACCCACCAGCACATCCCCTATCGTGAGAACAAGAACCTCACGGGGACGGCGCGGTACGCC
  TCCATCAACACGCACCTTGGAATTGAACAATCCCGAAGAGATGACTTGGAGTCTCTGGGC
  TACGTGCTAATGTACTTCAACCTGGGCTCTCTCCCCTGGCAGGGGCTGAAGGCTGCCACC
  AAGAGACAGAAATACGAAAGGATTAGCGAGAAGAAAATGTCCACCCCCATCGAAGTGTTG
  TGTAAAGGCTACCCTTCCGAATTTGCCACATACCTGAATTTCTGCCGTTCCTTGCGTTTT
  GACGACAAGCCTGACTACTCGTACCTGCGGCAGCTTTTCCGGAATCTGTTCCATCGCCAG
  GGCTTCTCCTATGACTACGTGTTCGACTGGAACATGCTCAAATTTGGTGCCAGCCGGGCC
  GCCGATGACGCCGAGCGGGAGCGCAGGGACCGAGAGGAGCGGCTGAGACACTCGCGGAAC
  CCGGCTACCCGCGGCCTCCCTTCCACAGCCTCCGGCCGCCTGCGGGGGACGCAGGAAGTG
  GCTCCCCCCACACCCCTCACCCCTACCTCACACACGGCTAACACCTCCCCCCGGCCCGTC
  TCCGGCATGGAGAGAGAGCGGAAAGTGAGTATGCGGCTGCACCGCGGGGCCCCCGTCAAC
  ATCTCCTCGTCCGACCTCACAGGCCGACAAGATACCTCTCGCATGTCCACCTCACAGAAT
  AGCATTCCTTTCGAACACCACGGCAAGTAG
  
  Clone variation with respect to NM_139062.2
  113 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_139062 unedited
  CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCTCCTTTAGGC
  AGCTGAAAGGGGATTTAGGCCCGGAAGATCCGAGTCCATCCGCGGCGGGGAGAGGGCAAGCGGGACCGGT
  AGGGGCCGGAGCAGCGGCGGCGGCGCTCGGACTGTCCCATCCGCCCCGTATTGAGGCGCTGGGAGCGGCG
  GGGCGACAGGAAAGCGATGGTGAAAGCGGGGCCGTGAGGGGGGGCGGGAGCCGGGGAGCCGGGACCTGTA
  GTATCGGCAGCAGCGGCGCCCGCCTCCCCAGAAGTTCAGAACCCAGGAGCCGGCCGGGGAGGGGAGGGAG
  CGGAATCCGGGCCGCCGCCCGCCTGGGAGCCTTAAAATTCGGGACAAGGAACCGGCGGGGCCGGAGAAAT
  CGGCACCGGTTCCTTCGGAGCATTCTTATCTCGGTACGACATTGGCTGCAGGAAAAAAGGTTGCATCATG
  CTGGATGGGTGTCAAAACCAAAACCTCTGGGCTCCACTTGGAGCGCAAATTCACAATGTAGTGCGGGGGG
  GGTGTGGGACTCCCACTAACAAAGGGCGGGGCGCAAGGGGGATCACCCTGTTAGGGGAGGGGCTCGGTGG
  GGACACTCTGAAAGCC
  
  Kinase domain sequence

  >SC323403 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CSAATGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGT
  CAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCTCCTTTAGGCAGCT
  GAAAGGGGATTTAGGCCCGGAAGATCCGAGTCCATCCGCGGCGGGGAGAGGGCAAGCGGGACCGGTAGGG
  GCCGGAGCAGCGGCGGCGGCGCTCGGACTGTCCCATCCGCCCCGT
  
• Restriction Sites: Please inquire
• ACCN: NM_139062
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139062.1, NP_620693.1
• RefSeq Size: 2106 bp
• RefSeq ORF: 1230 bp
• Locus ID: 1453
• UniProt ID: P48730
• Cytogenetics: 17q25.3
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Circadian rhythm - mammal, Gap junction, Hedgehog signaling pathway
• Gene Summary: This gene is a member of the casein kinase I (CKI) gene family whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein may also be involved in the regulation of apoptosis, circadian rhythm, microtubule dynamics, chromosome segregation, and p53-mediated effects on growth. The encoded protein is highly similar to the mouse and rat CK1 delta homologs. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (2) has an additional exon at the 3' end compared to transcript variant 1. This results in a shorter isoform (2) with a different C-terminus compared to isoform 1.