CKII alpha (CSNK2A1) (NM_001895) Human Untagged Clone

CAT#: SC323395

CSNK2A1 (untagged)-Kinase deficient mutant (K68M) of Human casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CKII alpha
• Synonyms: CK2A1; Cka1; Cka2; CKII; OCNDS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323395 sequence for NM_001895 edited (data generated by NextGen Sequencing)
  ATGTCGGGACCCGTGCCAAGCAGGGCCAGAGTTTACACAGATGTTAATACACACAGACCT
  CGAGAATACTGGGATTACGAGTCACATGTGGTGGAATGGGGAAATCAAGATGACTACCAG
  CTGGTTCGAAAATTAGGCCGAGGTAAATACAGTGAAGTATTTGAAGCCATCAACATCACA
  AATAATGAAAAAGTTGTTGTTATGATTCTCAAGCCAGTAAAAAAGAAGAAAATTAAGCGT
  GAAATAAAGATTTTGGAGAATTTGAGAGGAGGTCCCAACATCATCACACTGGCAGACATT
  GTAAAAGACCCTGTGTCACGAACCCCCGCCTTGGTTTTTGAACACGTAAACAACACAGAC
  TTCAAGCAATTGTACCAGACGTTAACAGACTATGATATTCGATTTTACATGTATGAGATT
  CTGAAGGCCCTGGATTATTGTCACAGCATGGGAATTATGCACAGAGATGTCAAGCCCCAT
  AATGTCATGATTGATCATGAGCACAGAAAGCTACGACTAATAGACTGGGGTTTGGCTGAG
  TTTTATCATCCTGGCCAAGAATATAATGTCCGAGTTGCTTCCCGATACTTCAAAGGTCCT
  GAGCTACTTGTAGACTATCAGATGTACGATTATAGTTTGGATATGTGGAGTTTGGGTTGT
  ATGCTGGCAAGTATGATCTTTCGGAAGGAGCCATTTTTCCATGGACATGACAATTATGAT
  CAGTTGGTGAGGATAGCCAAGGTTCTGGGGACAGAAGATTTATATGACTATATTGACAAA
  TACAACATTGAATTAGATCCACGTTTCAATGATATCTTGGGCAGACACTCTCGAAAGCGA
  TGGGAACGCTTTGTCCACAGTGAAAATCAGCACCTTGTCAGCCCTGAGGCCTTGGATTTC
  CTGGACAAACTGCTGCGATATGACCACCAGTCACGGCTTACTGCAAGAGAGGCAATGGAG
  CACCCCTATTTCTACACTGTTGTGAAGGACCAGGCTCGAATGGGTTCATCTAGCATGCCA
  GGGGGCAGTACGCCCGTCAGCAGCGCCAATATGATGTCAGGGATTTCTTTAGTGCCAACC
  CCTTCACCCCTTGGACCTCTGGCAGGCTCACCAGTGATTGCTGCTGCCAACCCCCTTGGG
  ATGCCTGTTCCAGCTGCCGCTGGCGCTCAGCAGTAA
  
  Clone variation with respect to NM_001895.3
  203 a=>t;204 a=>g;1070 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_001895 unedited
  CCGCCCGTCCAGCAACGGGCGGTAGGCGCGTACGGCGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAGAGCGGCCGCC
  GCCGCTGCCGCTTCCACCACAGTTTGAAGAAAACAGGTCTGAAACAAGGTCTTACCCCCAGCTGCTTCTG
  AACACAGTGACTGCCAGATCTCCAAACATCAAGTCCAGCTTTGTCCGCCAACCTGTCTGACATGTCGGGA
  CCCGTGCCAAGCAGGGCCAGAGTTTACACAGATGTTAATACACACAGACCTCGAGAATACTGGTGATTAC
  TGAGTCACATGTGGTGGAATGGGGAAATCAAGATGACTACCCAGCTGGTTCGAAAATTAGGCCGAGGTGA
  ATTACAGTTGAAGTATTTGAAGCCCATCACATCACAAATAATGAAAAAGTTGTTGGTTATGATTCTCAGC
  CAGTAAAAAAGAAGAAAATTAGCGTGAAATAAGATTTGGAGATTTGGAAGGAGGTCCCAACTCATCACCT
  GCAGGACATTGAAAAGACCTGGGTAAGAACCCCCGCCTGGTTTTTGAACCGTAACCACCGATTCAGCATT
  GTCCAACTTACGACATGGATTCGATTTACGGTAAATCGAGCCTGATATGCCAACTGGATTAGCAGAATTC
  AGCCTATGTCGATGACTGGCCGGAGACCACTAGACTGGTGCGATTAAACTGCCAATATGCAAGTTCGGAC
  TCAGTCGACATGACTGATGCAATTGATTGATGGGCGGCATTTT
  
  Kinase domain sequence

  >SC323395 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CKCTCGAATAGGCCGAGGTAATACAGTGAAGTATTTGAAGCCATCAACATCACAAATAATGAAAAAGTTG
  TTGTTATGATTCTCAAGCCAGTAAAAAAGAAGAAAATTAAGCGTGAAATAAAGATTTTGGAGAATTTGAG
  AGGAGGTCCCAACATCATCACACTGGCAGACATTGTAAAAGACCCTGTGTCACGAACCCCCGCCTTGGTT
  TTTGAACACGTAAACAACACAGACTTCAAGCAATTGTACCAGACG
  
• Restriction Sites: Please inquire
• ACCN: NM_001895
• Insert Size: 2680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001895.3, NP_001886.1
• RefSeq Size: 2732 bp
• RefSeq ORF: 1176 bp
• Locus ID: 1457
• UniProt ID: P68400
• Cytogenetics: 20p13
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Adherens junction, Tight junction, Wnt signaling pathway
• Gene Summary: Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. Multiple transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Apr 2018]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, 4 and 5 encode the same protein.