Upstream Binding Protein 1 (UBP1) (NM_001128161) Human Untagged Clone

CAT#: SC323127

UBP1 (untagged)-Human upstream binding protein 1 (LBP-1a) (UBP1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Upstream Binding Protein 1
• Synonyms: LBP-1a; LBP-1B; LBP1A; LBP1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC323127 representing NM_001128161.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCTGGGTGCTCAAGATGGACGAGGTGATCGAGTCCGGGCTGGTGCACGACTTCGACGCCAGCCTC
  TCGGGCATCGGGCAGGAACTGGGCGCCGGCGCTTACAGCATGAGTGATGTCTTGGCATTGCCCATTTTC
  AAGCAGGAAGATTCCAGCCTTCCATTGGATGGTGAAACAGAGCACCCACCCTTTCAGTATGTGATGTGT
  GCTGCAACGTCACCAGCAGTAAAACTGCATGATGAAACGCTTACTTATTTGAACCAAGGTCAGTCATAT
  GAAATTCGGATGCTGGATAATCGGAAAATGGGTGATATGCCTGAGATCAATGGAAAATTAGTAAAGAGC
  ATCATAAGGGTTGTATTCCATGACAGACGGCTACAATACACAGAGCATCAGCAACTTGAAGGATGGAAG
  TGGAATCGCCCAGGAGACAGACTTCTTGATTTAGATATTCCAATGTCTGTGGGAATAATTGACACAAGG
  ACGAATCCAAGCCAGTTAAATGCGGTTGAATTTCTGTGGGACCCAGCAAAACGCACCTCTGCTTTCATT
  CAGGTACACTGCATCAGCACAGAATTTACTCCACGGAAGCACGGAGGTGAAAAGGGAGTGCCCTTTAGG
  ATCCAGGTTGACACCTTTAAGCAGAATGAAAATGGAGAATACACAGATCATCTACACTCAGCTAGCTGC
  CAAATCAAAGTTTTTAAGCCTAAAGGTGCAGACAGGAAACAAAAAACTGACCGAGAGAAGATGGAGAAG
  AGAACAGCTCATGAAAAAGAAAAGTATCAGCCGTCCTATGATACCACAATCCTCACAGAGATGAGGCTT
  GAGCCTATAATTGAAGATGCAGTTGAACATGAGCAGAAAAAGTCCAGCAAGCGGACTTTGCCAGCAGAC
  TACGGTGATTCTCTGGCAAAGCGAGGCAGTTGTTCTCCGTGGCCCGATGCCCCCACAGCCTATGTGAAT
  AACAGCCCTTCCCCAGCGCCCACTTTCACCTCCCCACAGCAGAGCACTTGCAGTGTCCCAGACAGCAAT
  TCTTCTTCCCCAAATCATCAGGGAGATGGAGCTTCACAGACCTCTGGTGAACAAATTCAGCCTTCAGCT
  ACGATCCAGGAAACACAGCAATGGCTGCTCAAAAACAGATTCTCTTCCTACACAAGACTGTTCTCTAAT
  TTTTCAGGTGCCGACTTATTAAAACTGACAAAGGAGGATTTAGTTCAAATTTGTGGTGCAGCCGATGGA
  ATTCGGCTCTATAATTCACTGAAGTCAAGGTCGGTTAGACCCCGTTTAACCATCTATGTCTGCCGGGAG
  CAGCCAAGCAGCACAGTGCTGCAAGGGCAGCAGCAAGCTGCAAGCAGTGCAAGCGAGAATGGCAGTGGG
  GCACCCTATGTTTATCATGCAATCTACTTGGAAGAAATGATTGCCTCAGAAGTTGCTCGAAAACTTGCG
  CTGGTGTTTAATATCCCTCTCCACCAAATTAATCAGGTTTACAGACAGGGTCCCACCGGTATTCACATT
  CTTGTTAGTGATCAGATGGTTCAGAATTTTCAAGATGAGAGTTGTTTTTTATTCTCCACAGTAAAAGCT
  GAAAGTAGTGATGGCATCCACATAATTTTGAAGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001128161
• Insert Size: 1623 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001128161.1
• RefSeq Size: 3781 bp
• RefSeq ORF: 1623 bp
• Locus ID: 7342
• UniProt ID: Q9NZI7
• Cytogenetics: 3p22.3
• Protein Families: Transcription Factors
• MW: 60.5 kDa
• Gene Summary: Functions as a transcriptional activator in a promoter context-dependent manner. Modulates the placental expression of CYP11A1. Involved in regulation of the alpha-globin gene in erythroid cells. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with TFCP2 (By similarity). Involved in regulation of the alpha-globin gene in erythroid cells. Binds strongly to sequences around the HIV-1 initiation site and weakly over the TATA-box. Represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA-box.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1 and 3 encode the same isoform (LBP-1b).