LSS (NM_002340) Human Untagged Clone

CAT#: SC322588

LSS (untagged)-Human lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (LSS), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LSS
• Synonyms: APMR4; CTRCT44; HYPT14; OSC
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002340 edited
  CCACGCGTCCGCCCACGCGTCCGGTAGAGCACTGCAGCAGCAATGACGGAGGGCACGTGT
  CTGCGGCGCCGAGGGGGCCCCTACAAGACCGAGCCCGCCACCGACCTCGGCCGCTGGCGA
  CTCAACTGCGAGAGGGGCCGGCAGACGTGGACCTACCTGCAGGACGAGCGCGCCGGCCGC
  GAGCAGACCGGCCTGGAAGCCTACGCCCTGGGGCTGGACACCAAGAATTACTTTAAGGAC
  TTGCCCAAAGCCCACACCGCCTTTGAGGGGGCTCTGAACGGGATGACATTTTACGTGGGG
  CTGCAGGCTGAGGATGGGCACTGGACGGGTGATTATGGTGGCCCACTTTTCCTCCTGCCA
  GGCCTCCTGATCACTTGCCACGTGGCACGCATCCCTCTGCCAGCCGGATACAGAGAAGAG
  ATTGTGCGGTACCTGCGGTCAGTGCAGCTCCCTGACGGTGGCTGGGGCCTGCACATTGAG
  GATAAGTCCACCGTGTTTGGGACTGCGCTCAACTATGTGTCTCTCAGAATTCTGGGTGTT
  GGGCCTGACGATCCTGACCTGGTACGAGCCCGGAACATTCTTCACAAGAAAGGTGGTGCT
  GTGGCCATCCCCTCCTGGGGGAAGTTCTGGCTGGCTGTCCTGAATGTTTACAGCTGGGAA
  GGCCTCAATACCCTGTTCCCAGAGATGTGGCTGTTTCCTGACTGGGCACCGGCACACCCC
  TCCACACTCTGGTGCCACTGCCGGCAGGTGTACCTGCCCATGAGCTACTGCTACGCCGTT
  CGGCTGAGTGCCGCGGAAGACCCGCTGGTCCAGAGCCTCCGCCAGGAGCTCTATGTGGAG
  GACTTCGCCAGCATTGACTGGCTGGCGCAGAGGAACAACGTGGCCCCCGACGAGCTGTAC
  ACGCCCCACAGCTGGCTGCTCCGCGTGGTATATGCGCTCCTCAACCTGTATGAGCACCAC
  CACAGTGCCCACCTGCGGCAGCGGGCCGTGCAGAAGCTGTATGAACACATTGTGGCCGAC
  GACCGATTCACCAAGAGCATCAGCATCGGCCCGATCTCGAAAACCATCAACATGCTTGTG
  CGCTGGTATGTGGACGGGCCCGCCTCCACTGCCTTCCAGGAGCATGTCTCCAGAATCCCG
  GACTATCTCTGGATGGGCCTTGACGGCATGAAAATGCAGGGCACCAACGGCTCACAGATC
  TGGGACACCGCATTCGCCATCCAGGCTCTGCTTGAGGCGGGCGGGCACCACAGGCCCGAG
  TTTTCGTCCTGCCTGCAGAAGGCTCATGAGTTCCTGAGGCTCTCACAGGTCCCAGATAAC
  CCTCCCGACTACCAGAAGTACTACCGCCAGATGCGCAAGGGTGGCTTCTCCTTCAGTACG
  CTGGACTGCGGCTGGATCGTTTCTGACTGCACGGCTGAGGCCTTGAAGGCTGTGCTGCTC
  CTGCAGGAGAAGTGTCCCCATGTCACCGAGCACATCCCCAGAGAACGGCTCTGCGATGCT
  GTGGCTGTGCTGCTGAACATGAGAAATCCAGATGGAGGGTTCGCCACCTATGAGACCAAG
  CGTGGGGGGCACTTGCTGGAGCTGCTGAACCCCTCGGAGGTCTTCGGGGACATCATGATT
  GACTACACCTATGTGGAGTGCACCTCAGCCGTGATGCAGGCGCTTAAGTATTTCCACAAG
  CGTTTCCCGGAGCACAGGGCAGCGGAGATCCGGGAGACCCTCACGCAGGGCTTAGAGTTC
  TGTCGGCGGCAGCAGAGGGCCGATGGCTCCTGGGAAGGCTCCTGGGGAGTTTGCTTCACC
  TACGGCACCTGGTTTGGCCTGGAGGCCTTCGCCTGTATGGGGCAGACCTACCGAGATGGG
  ACTGCCTGTGCAGAGGTCTCCCGGGCCTGTGACTTCCTGCTGTCCCGGCAGATGGCAGAC
  GGAGGCTGGGGGGAGGACTTTGAGTCCTGCGAGGAGCGGCGTTATGTGCAGAGTGCCCAG
  TCCCAGATCCATAACACATGCTGGGCCATGATGGGGCTGATGGCCGTTCGGCATCCTGAC
  ATCGAGGCCCAGGAGAGAGGAGTCCGGTGTCTACTTGAGAAACAGCTCCCCAATGGCGAC
  TGGCCGCAGGAAAACATTGCTGGGGTCTTCAACAAGTCCTGTGCCATCTCCTACACGAGC
  TACAGGAACATCTTCCCCATCTGGGCCCTCGGCCGCTTCTCCCAGCTGTACCCTGAGAGA
  GCCCTTGCTGGCCACCCCTGAGAACATGCCTACCTGCTGGGTGCCGTCTGTGCGTTCCAG
  TGAGGCCAAGGGGTCCTGGCCGGGTTGGGGAGCCCTCCCATAACCCTGTCTTGGGCTCCA
  ACCCCTCAACCTCTATCTCATAGATGTGAATCTGGGGGCCAGGCTGGAGGCAGGGATGGG
  GACAGGGTGGGTGGCTTAGACTCTTGATTTTTACTGTAGGTTCATTTCTGAAAGTAGCTT
  GTCGGGCTTGGGTGAGGAAGGGGGCACAGGAGCCGTGACCCCTGAGGAGGCACAGCGCCT
  TCTGCCACCTCTGGGCACGGCCTCAAGGTAGTGAGGCTAGGAGGTTTTTTCTGACCAATA
  GCTGAGTTCTTGGGAGAGGAGCAGCTGTGCCTGTGTGATTCCTTAGTGTCGAGTGGGCTC
  TGGGCTGGGGTCGGCCCTGGGCAGGCTTCTCCTGCACCTTTTGTCTGCTGGGCTGAGGGA
  CACGAGGGCAACCCTGTGACAATGGCAGGTAGTGTGCATCCGTGAATAGCCCAGTGCGGG
  GGTTGCTCATGGAGCATCCTGAGGCCGTGCAGCAGGGAGCCCCATGCCCCTGGGTCGTGA
  GCTTGCCTGCGTATGGGGTGGTGTCATGGAGCCTCATGCCCCTGGGTCGTGAGCTCGCCT
  GAGTATGGGGTGGTGTCATGGAGCCGCATACCCCTGGGTTGTGAGCTCGCCTGCATATGC
  AGGGTCTGTCATGGAACATCCCAAGTCTGTGCAGCAGGGAGCCCCATGCCCCTGGGACAT
  GAACCCACCTGCGTGGAATGCTGTTTGTGAGGTGTCTACAGGGTTTATAGTAGTCTTGTG
  GACACAGAAATGCACAGGGGACACTTACGGACACAGAAATGCACAGGGGAGGCCGAGCAT
  AACCAGGGGTGAGGGGCAGGCAGCAGTTGTAGTTACTGCCGCGGGGCACTGCTATGTGCA
  GGGACAGCCAGCGCCCAGCCCATCACCACTCCCTGGGCTGGCTGGCAGGTATGGCACCCT
  GGGAGCCCGGCATATACCCAGGGCACCCCTACGGCTGCCGCCAGTCTCATGCCCAGGTGG
  GTGCTCTGGGCTGGAGCGAGGGCCAGGTTTTGGGCCGAGGCTTCCCCAGGCAATCCTGTG
  AGCTCCCTTCTAGCCTCTGACCCAGTCTGGTCTGGCTTGCATGGATGTAGGGCTTGGGGT
  GGGAAGTTCAGGTCCTGGCTTTGCCTTTGCCTGATGTGGATGAGCAGCTCACATGCTCAG
  GGCCACCTGAGACTGTCACTGCTCTCCCCTGGCTACTGGGAGGAGTCACTGAGAGCTTCG
  TTACCCCTGCTGCCTTGCCCAGGGCACACCCTATACCTCCTCATCTGCTCTTCCCCTCCC
  TGCCGCCTTCTGGGCAGGTAGCAGTCCCTGGCCTCTCCCCCTGGCTGATCACTCTCCCTC
  AGGCAGTGGAGATCTGCGTCTGGACACCCTCAGATCCTGTCATTGCCTGCCCAGAGTCCT
  TCAGGGGCACCCCTCTGCCTTGGTGTGCGGTCCAGGGCTCTCACCCAGGTGCCGCACCCT
  CTGGGGTCTTCTGTCCAGCTCCCTTGCCCCATGTGCTGTCACTGACTCTCCTTGGGACTC
  GCCTGCCTGCTCAGAGCCCTGCAGGGCTTGGTCAGCTGCCTGTTCAGTGTCAACACTTCC
  CTGCACATCTTAAAACTGGGCTTTATTTTCGCTGAAGGAACTGTGTTGGGACCCTTGACA
  TCTGTCAGGTTTGCACATGCTGTTTTTTTTTCTCAGCCCACGTGTTCTCCCCCACGTGGG
  GTAGCAGCAGGACAGACAGTGAATCACAGAGTCTGCCCTGAGCAGAGGCTGCTGTCCCTG
  GGACTCCTAGCCATGGTCAGACTGTACAAAACGGTTTTCCAGAAATGAAATGTAAATCCA
  TTTTTATACTGAAAATGTTACTGAAAGTCACTTTTATGAGCATCTGCCTTAATAAACAGA
  CATTGATTCCCTTATCAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_002340
• Insert Size: 4300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002340.3, NP_002331.3
• RefSeq Size: 4225 bp
• RefSeq ORF: 2199 bp
• Locus ID: 4047
• UniProt ID: P48449
• Cytogenetics: 21q22.3
• Domains: prenyltrans
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Steroid biosynthesis
• Gene Summary: The protein encoded by this gene catalyzes the conversion of (S)-2,3 oxidosqualene to lanosterol. The encoded protein is a member of the terpene cyclase/mutase family and catalyzes the first step in the biosynthesis of cholesterol, steroid hormones, and vitamin D. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Feb 2009]
  Transcript Variant: This variant (1) differs in the 3' UTR, compared to variant 2. Variants 1 and 2 encode the same protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from genomic and transcript sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.