PYCR2 (NM_013328) Human Untagged Clone

CAT#: SC322308

PYCR2 (untagged)-Human pyrroline-5-carboxylate reductase family, member 2 (PYCR2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PYCR2
• Synonyms: HLD10; P5CR2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322308 GAATAGGGTTGCACCATCCCAGAAGCTGCTGTTAGCTCGCCGGTCCTCGGCACGCCGCCC
  GTTCGCCCCTGCGCTGTCCGCCCTTCCCCTAGCGTTACTTCCGGTCCCTCGCTGAGGGGG
  TTCGTGCGGCTCCCAGGAGGCGTGAACCGCGGACCATGAGCGTGGGCTTCATCGGGGCCG
  GCCAGCTGGCCTATGCTCTGGCGCGGGGCTTCACGGCCGCAGGCATCCTGTCGGCTCACA
  AGATAATAGCCAGCTCCCCAGAAATGAACCTGCCCACGGTGTCCGCGCTCAGGAAGATGG
  GTGTGAACCTGACACGCAGCAACAAGGAGACGGTGAAGCACAGCGACGTCCTGTTTCTGG
  CTGTGAAGCCACATATCATCCCCTTCATCCTGGATGAGATTGGGGCCGACGTGCAAGCCA
  GACACATCGTGGTCTCCTGTGCGGCTGGTGTCACCATCAGCTCTGTGGAGAAGAAGCTGA
  TGGCATTCCAGCCAGCCCCCAAAGTGATTCGCTGCATGACCAACACACCTGTGGTAGTGC
  AGGAAGGCGCTACAGTGTACGCCACGGGCACCCATGCCCTGGTGGAGGATGGGCAGCTCC
  TGGAGCAGCTCATGAGCAGCGTGGGCTTCTGCACTGAGGTGGAAGAGGACCTCATCGATG
  CCGTCACGGGGCTCAGTGGCAGCGGGCCTGCCTATGCATTCATGGCTCTGGACGCATTGG
  CTGATGGTGGGGTGAAGATGGGTTTGCCACGGCGCCTGGCAATCCAACTCGGGGCCCAGG
  CTTTGCTGGGAGCTGCCAAGATGCTGCTGGACTCGGAGCAGCATCCATGCCAGCTTAAGG
  ACAATGTCTGCTCCCCTGGGGGAGCCACCATCCACGCCCTGCACTTTCTAGAGAGTGGGG
  GCTTCCGCTCTCTGCTCATCAATGCAGTTGAGGCCTCCTGTATCCGAACACGAGAGCTAC
  AGTCCATGGCCGACCAAGAAAAGATCTCCCCAGCTGCCCTTAAGAAGACCCTCTTAGACA
  GAGTGAAGCTGGAATCCCCCACAGTCTCCACACTGACCCCCTCCAGCCCAGGGAAGCTCC
  TCACAAGAAGCCTGGCCCTGGGAGGCAAGAAGGACTAAGGCAGCATCTGTCCCCTCTGTG
  ATTCAGAGCCCTTAGTTGAGAGCCCCTGCCGCCCCTGCCACCCCCCTGCCCCGCTCCCAC
  CATTGCCCCTCCTCAGCTGTGCAAGGAGAAAGCATGCTTAGGAAGTTTTCAGGTCCTTGT
  GATAAAACCTCCTTAAATCTGTTCAGACCAAGCAATGCGAGCTTCCTCTCCTGTCCCATG
  TTGGAAGTTGCTCTGAAGGGGTGGTAGATGCTGGAAGCCAGACACAACCCTGCGTACGCT
  GCTCAGTTGGTGGAGACTGGGGCTGGGACTGGAGTCAGCCCAGCTGGGAGGAGGGGCTGG
  GGAGGATCTGCAGCTGAAGCCCGAGGCAGGGTTGGTGTGATGCCAAGGCAAAGTGGTGAG
  GAGAAAACAGGAAACGGGCTTTCTCTGAATTGGTAAATGGGAAAGAAGTGAGCAACTTAA
  GATTGTCACAATTAATCACAAGTGTACAGGATTAGACTGGGTTTATATTTAACTCTTGCT
  TCATAGGTGTACCATTTAAAGAGTGTTATTTAATGCTAAGTTTAACTGCTTTAATAAAGT
  TTATTTTTAAATAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_013328
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013328.2, NP_037460.2
• RefSeq Size: 1708 bp
• RefSeq ORF: 963 bp
• Locus ID: 29920
• UniProt ID: Q96C36
• Cytogenetics: 1q42.12
• Domains: P5CR
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• Gene Summary: This gene belongs to the pyrroline-5-carboxylate reductase family. The encoded mitochondrial protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).