GATM (NM_001482) Human Untagged Clone

CAT#: SC321307

GATM (untagged)-Human glycine amidinotransferase (L-arginine:glycine amidinotransferase) (GATM), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GATM
• Synonyms: AGAT; AT; CCDS3; FRTS1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001482.2 CGGGAAGGCTTGGACCGACGCGGCCCAGAGGCCAGGAACATTCCGCGCGTGGACCAGCCG
  GGCCAGGGCGATGCTGCGGGTGCGGTGTCTGCGCGGCGGGAGCCGCGGCGCCGAGGCGGT
  GCACTACATCGGATCTCGGCTTGGACGAACCTTGACAGGATGGGTGCAGCGAACTTTCCA
  GAGCACCCAGGCAGCTACGGCTTCCTCCCGGAACTCCTGTGCAGCTGACGACAAAGCCAC
  TGAGCCTCTGCCCAAGGACTGCCCTGTCTCTTCTTACAACGAATGGGACCCCTTAGAGGA
  AGTGATAGTGGGCAGAGCAGAAAACGCCTGTGTTCCACCGTTCACCATCGAGGTGAAGGC
  CAACACATATGAAAAGTACTGGCCATTTTACCAGAAGCAAGGAGGGCATTATTTTCCCAA
  AGATCATTTGAAAAAGGCTGTTGCTGAAATTGAAGAAATGTGCAATATTTTAAAAACGGA
  AGGAGTGACAGTAAGGAGGCCTGACCCCATTGACTGGTCATTGAAGTATAAAACTCCTGA
  TTTTGAGTCTACGGGTTTATACAGTGCAATGCCTCGAGACATCCTGATAGTTGTGGGCAA
  TGAGATTATCGAGGCTCCCATGGCATGGCGTTCACGCTTCTTTGAGTACCGAGCGTACAG
  GTCAATTATCAAAGACTACTTCCACCGTGGCGCCAAGTGGACAACAGCTCCTAAGCCCAC
  AATGGCTGATGAGCTTTATAACCAGGATTATCCCATCCACTCTGTAGAAGACAGACACAA
  ATTGGCTGCTCAGGGAAAATTTGTGACAACTGAGTTTGAGCCATGCTTTGATGCTGCTGA
  CTTCATTCGAGCTGGAAGAGATATTTTTGCACAGAGAAGCCAGGTTACAAACTACCTAGG
  CATTGAATGGATGCGTAGGCATCTTGCTCCAGACTACAGAGTGCATATCATCTCCTTTAA
  AGATCCCAATCCCATGCATATTGATGCTACCTTCAACATCATTGGACCTGGTATTGTGCT
  TTCCAACCCTGACCGACCATGTCACCAGATTGATCTTTTCAAGAAAGCAGGATGGACTAT
  CATTACTCCTCCAACACCAATCATCCCAGACGATCATCCACTCTGGATGTCATCCAAATG
  GCTTTCCATGAATGTCTTAATGCTAGATGAAAAACGTGTTATGGTGGATGCCAATGAAGT
  TCCAATTCAAAAGATGTTTGAAAAGCTGGGTATCACTACCATTAAAGTTAACATTCGTAA
  TGCCAATTCCCTGGGAGGAGGCTTCCATTGCTGGACCTGCGATGTCCGGCGCCGAGGCAC
  CTTACAGTCCTACTTGGACTGAACAGGCCTGATGGAGCTTGTGGCTGGCCTCAGATACAC
  CTAAGAAGCTTAGGGGCAAGGTTCATTCTCCTGCTTTAAAAAGTGCATGAACTGTAGTGC
  TTTAAACAATCATCTCCTTAACAGGGGTCGTAAGCCTGGTTTGCTTCTATTACTTTTCTT
  TGACATAAAGAAAATAACTTCTGCTAGGTATTACTCTCTACTCCTAAAGTTATTTACTAT
  TTGGCTTCAAGTATAAAATTTTGGTGAATGTGTACCAAGAAAAAATTAGTCACCTGAGTA
  ACTTGGCCACTAATAATTAACCATCTACCTCTGTTTTTAATTTTCTTTCCAAAAGGCAGC
  TTGAAATGTTGGTCCTAATCTTAATTTTTTTTCCTCTTCTATAGACTTGAGAATGTTTTT
  CTCTAAATGAGAGAAAGACTTAGAATGTACACAGATCCAAAATAGAATCAGATTATCTCT
  TTTTTTCTAAAGGAGAGAAAGACTTAGAACATACACAGATCCTAAGTAGAACCAGGTAAT
  TGTCTCTTTTTCTAATAAGGAATTTGGGTAATTTTTAATTTTTTGTTTTTTAAAAAATAA
  CCTAGACTATGCAAAACATCAAAGTGAATTTTCCATGAATGTTTTTAATATTCTCATCTC
  AACATTGTGATATATGCTACTAAAAACCTTTTCATATACATCTTACCTCATTTCAAGTGA
  ATTATTTTAATCTTTTTCTCTCTTTCCAAAAATTTAGGAATGTTTAGTGTAATTGGATTT
  CGCTATCAGTTCCCATCCTTAAGTTTTGATATTCAATATCTGATAGATACACTGCATCTT
  TGGTCATCTAAGATTTGTTTACAAATGTGCAAATTATTTAGAGCATAGACTTTATAAGCA
  TTAAAAAAAACTAATGGAGGTAAAACCTAAATGCGATGTGAAATAATTTTAGTGTTGATA
  CCGTATGTGTATTTTTATTCTAATAAACTTTTGTGTTCCAGAAAAAAAAAAAAAAAAAAA
  AA
• Restriction Sites: Please inquire
• ACCN: NM_001482
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001482.2, NP_001473.1
• RefSeq Size: 2602 bp
• RefSeq ORF: 1272 bp
• Locus ID: 2628
• UniProt ID: P50440
• Cytogenetics: 15q21.1
• Domains: Amidinotransf
• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Glycine, serine and threonine metabolism, Metabolic pathways
• Gene Summary: This gene encodes a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis, whereby it catalyzes the transfer of a guanido group from L-arginine to glycine, resulting in guanidinoacetic acid, the immediate precursor of creatine. Mutations in this gene cause arginine:glycine amidinotransferase deficiency, an inborn error of creatine synthesis characterized by cognitive disability, language impairment, and behavioral disorders. [provided by RefSeq, Jul 2008]