TFIP11 (NM_012143) Human Untagged Clone

CAT#: SC320803

TFIP11 (untagged)-Human tuftelin interacting protein 11 (TFIP11), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TFIP11
• Synonyms: bK445C9.6; NTR1; Spp382; STIP; STIP-1; TIP39
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012143.2 GGCGTCTTCTCGCGCGGATGGTGAAGCTGAATTTGGTACGCTGGACCCTCGGACAGACAG
  CAAGAGAGAGACAAGCCCGAGGCCTGACTTCTAGCACTCCTGAGTTTGAGCCCTATGAGC
  AGTGGAACCTAGGGACCACCAAGGATCCAGATATAATTCTTTCCTCAGTAATAGAAAAAA
  GAATCTGGGATCTAAGAAGATACCTACTCATTTTCTAAGTGACTGGCCATGTCATTGTCC
  CACTTATACCGGGATGGGGAAGGCCGCATTGATGATGATGATGACGAGCGGGAGAACTTT
  GAGATCACTGACTGGGATCTCCAGAATGAGTTCAACCCCAACCGACAGCGCCACTGGCAG
  ACCAAGGAAGAAGCCACCTACGGGGTGTGGGCAGAGCGAGACTCGGATGATGAGAGGCCC
  AGCTTTGGAGGCAAACGGGCCCGTGACTACTCTGCGCCAGTCAACTTCATCAGCGCAGGG
  CTCAAGAAAGGGGCAGCGGAGGAGGCAGAGTCGGAAGATTCTGATGACGAAGAGAAACCT
  GTTAAGCAGGACGACTTTCCTAAGGATTTTGGACCAAGGAAGCTAAAAACGGGTGGCAAT
  TTTAAGCCCAGCCAGAAAGGTTTTGCAGGAGGAACCAAATCTTTCATGGACTTCGGCAGC
  TGGGAAAGACACACAAAAGGAATTGGACAGAAGCTTCTTCAGAAGATGGGCTACGTCCCT
  GGACGGGGCCTCGGGAAGAATGCACAAGGTATCATTAACCCAATTGAAGCCAAGCAGAGA
  AAGGGAAAAGGTGCTGTGGGGGCTTATGGATCCGAGCGCACCACTCAGTCCATGCAAGAC
  TTCCCTGTGGTTGACTCAGAGGAAGAAGCTGAAGAGGAGTTTCAGAAGGAGCTGAGCCAG
  TGGAGGAAAGACCCAAGTGGAAGCAAGAAGAAGCCCAAATACTCTTACAAGACCGTGGAA
  GAGTTGAAGGCCAAGGGCAGGATTAGCAAGAAGCTCACTGCTCCCCAGAAGGAACTTTCT
  CAAGTCAAGGTCATAGACATGACAGGCCGGGAGCAGAAGGTCTACTACAGCTACAGTCAG
  ATCAGCCACAAGCACAACGTTCCCGATGATGGGCTGCCGCTACAGTCCCAACAGCTGCCA
  CAGTCTGGCAAAGAGGCCAAGGCCCCCGGCTTCGCGCTGCCCGAGCTGGAGCACAACCTG
  CAGCTGCTCATCGACCTCACGGAGCAGGAGATCATCCAGAATGACCGGCAGCTACAGTAT
  GAGCGGGACATGGTGGTCAACCTCTTCCATGAGCTGGAGAAGATGACCGAGGTCCTGGAC
  CACGAGGAGCGGGTCATCTCGAACCTCAGCAAGGTCCTGGAGATGGTGGAGGAGTGCGAG
  CGGCGGATGCAGCCCGACTGCAGCAACCCCCTCACCCTGGACGAGTGTGCCCGCATCTTC
  GAAACCCTGCAGGACAAGTACTATGAGGAGTACAGGATGTCCGACCGTGTGGACCTTGCT
  GTGGCCATCGTCTATCCACTCATGAAGGAGTACTTCAAGGAGTGGGATCCCCTCAAAGAC
  TGCACTTATGGCACCGAGATCATCTCTAAGTGGAAAAGCCTCCTAGAGAATGACCAGCTC
  TTGTCCCATGGCGGACAGGACCTCTCAGCAGATGCCTTTCACAGGTTGATATGGGAAGTC
  TGGATGCCTTTTGTTCGAAATATTGTCACCCAGTGGCAGCCAAGGAACTGTGACCCGATG
  GTGGACTTTTTGGATAGTTGGGTGCACATTATTCCTGTGTGGATCTTAGATAACATACTG
  GACCAACTCATCTTCCCCAAGCTGCAAAAGGAGGTGGAAAACTGGAACCCGCTCACAGAC
  ACTGTTCCCATCCACTCTTGGATCCACCCATGGCTGCCCCTTATGCAGGCACGGCTGGAG
  CCACTCTATTCCCCCATCCGTAGTAAGCTGTCCAGCGCCCTGCAGAAGTGGCACCCCAGC
  GACTCCTCTGCCAAGCTCATCCTCCAGCCCTGGAAGGATGTCTTCACTCCTGGCTCCTGG
  GAAGCATTCATGGTCAAAAACATAGTGCCCAAGCTGGGGATGTGTCTTGGTGAGCTAGTC
  ATTAACCCCCACCAGCAGCACATGGATGCATTCTATTGGGTGATTGACTGGGAAGGGATG
  ATCTCTGTCTCTAGCCTGGTGGGACTTCTTGAAAAGCACTTCTTCCCCAAGTGGCTTCAG
  GTGCTGTGCTCTTGGCTCAGTAACAGCCCAAATTATGAGGAGATCACCAAGTGGTACCTG
  GGTTGGAAGTCGATGTTCTCAGACCAAGTGCTGGCACATCCATCTGTCAAGGACAAATTT
  AATGAGGCACTTGATATCATGAACCGGGCGGTGTCCTCCAACGTTGGTGCCTACATGCAG
  CCAGGAGCACGGGAGAACATTGCCTATCTCACCCACACGGAGCGGAGGAAGGACTTCCAG
  TACGAGGCCATGCAGGAGAGGCGGGAGGCTGAGAACATGGCTCAGAGGGGCATTGGCGTG
  GCCGCTAGCTCTGTGCCCATGAACTTTAAGGACCTCATTGAGACCAAGGCTGAGGAGCAC
  AACATTGTCTTCATGCCCGTCATTGGGAAGCGACACGAAGGGAAGCAGCTCTACACCTTT
  GGCCGCATTGTGATCTACATCGACCGGGGAGTGGTCTTTGTCCAGGGCGAGAAGACGTGG
  GTGCCCACCTCACTGCAGAGCCTGATCGACATGGCCAAGTGAACTGTGGCAGGTCCAGAA
  CCAGTCAGAGACTTGCCCCTAAGAGGGATGTACTGTAAATAAACAGTATTTTTAGATCAC
  CTTAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_012143
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012143.2, NP_036275.1
• RefSeq Size: 2864 bp
• RefSeq ORF: 2514 bp
• Locus ID: 24144
• UniProt ID: Q9UBB9
• Cytogenetics: 22q12.1
• Domains: G-patch
• Gene Summary: This gene encodes a protein component of the spliceosome that promotes the release of the lariat-intron during late-stage splicing through the recruitment of a pre-mRNA splicing factor called DEAH-box helicase 15. The encoded protein contains a G-patch domain, a hallmark of RNA-processing proteins, that binds DEAH-box helicase 15. This protein contains an atypical nuclear localization sequence as well as a nuclear speckle-targeting sequence, enabling it to localize to distinct speckled regions within the cell nucleus. Polymorphisms in this gene are associated with dental caries suggesting a role in amelogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]
  Transcript Variant: This variant (2) lacks an exon in the 5' UTR, compared to variant1. Variants 1-6 encode the same isoform (1).