PCYT2 (NM_002861) Human Untagged Clone

CAT#: SC320786

PCYT2 (untagged)-Human phosphate cytidylyltransferase 2, ethanolamine (PCYT2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCYT2
• Synonyms: ET; SPG82
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002861.1 GCCTGGCGGGGCGGGCGGCGGCGCTCGGAGTCGCCGGGAGCTGCCAGGCTGTCCGCGCCG
  CCGCTGCGGGGCCATGATCCGGAACGGGCGCGGGGCTGCAGGCGGCGCAGAGCAGCCGGG
  CCCGGGGGGCAGGCGCGCCGTGAGGGTGTGGTGCGATGGCTGCTATGACATGGTGCATTA
  CGGCCACTCCAACCAGCTGCGCCAGGCACGGGCCATGGGTGACTACCTCATCGTAGGCGT
  GCACACCGATGAGGAGATCGCCAAGCACAAGGGGCCCCCGGTGTTCACTCAGGAGGAGAG
  ATACAAGATGGTGCAGGCCATCAAATGGGTGGACGAGGTGGTGCCAGCGGCTCCCTACGT
  CACTACACTAGAGACCCTGGACAAATACAACTGTGACTTCTGTGTTCACGGCAATGACAT
  CACCCTGACTGTAGATGGCCGGGACACCTATGAGGAAGTAAAGCAGGCTGGGAGGTACAG
  AGAATGCAAGCGCACGCAAGGGGTGTCCACCACAGACCTCGTGGGCCGCATGCTGCTGGT
  AACCAAAGCCCATCACAGCAGCCAGGAGATGTCCTCTGAGTACCGGGAGTATGCAGACAG
  TTTTGGCAAGTGCCCTGGTGGGCGGAACCCCTGGACCGGGGTATCCCAGTTCCTGCAGAC
  ATCTCAGAAGATCATCCAGTTTGCTTCTGGGAAGGAGCCCCAGCCAGGGGAGACAGTCAT
  CTATGTGGCTGGTGCCTTCGACCTGTTCCACATCGGGCATGTGGACTTCCTGGAGAAGGT
  GCACAGGCTGGCAGAGAGGCCCCACATCATCGCGGGCTTACACTTTGACCAGGAGGTCAA
  TCACTACAAGGGGAAGAACTACCCCATCATGAATCTGCATGAACGGACTCTGAGCGTGCT
  GGCCTGCCGGTACGTGTCAGAAGTGGTGATTGGAGCCCCGTACGCGGTCACAGCAGAGCT
  CCTAAGTCACTTCAAGGTGGACCTGGTGTGTCACGGCAAGACAGAAATTATCCCTGACAG
  GGATGGCTCCGACCCATACCAGGAGCCCAAGAGAAGGGGCATCTTCCGTCAGATTGACAG
  TGGCAGCAACCTCACCACAGACCTCATCGTCCAGCGGATCATCACCAACAGGTTGGAGTA
  TGAGGCGCGAAACCAGAAGAAGGAAGCCAAGGAGCTGGCCTTCCTGGAGGCTGCCAGGCA
  GCAGGCGGCACAGCCCCTGGGGGAGCGCGATGGTGACTTCTAACCTGGCAGAGGCCCTGG
  CCGGCCCTCCCCCTGCTCTGCTTCTGCGCCTTCTGCGTTTGGACATAGGACTCTGCAGGG
  CCGCCCTCTCTAACTGGCCTGGCTCTGGAAGGGCTGGTGAGGACTCTGCCTCCTTGCCTG
  CCTACAAGGTGCCTGGTTTGCAGCAGGCTCTCCGCTCTTTCCAGCAAAGCTGCTCAGAGA
  GGGTGTCCAGCACAGTGGAGAGGCCGGAAGTGAGACGGGCAGACGGCACCTGCAGCCTGA
  AACGCACCGCTCCTGCGTGCGCCCCCACCTGGTCCCCGGATGCCCCCACCACCTGGACAG
  AGGCCACACTGACTGCCCACCCAGCTGTGGCGGGAGGTGCAGAGCAGAGGGCTTTAGGGA
  GCAGTGACTGCGGTCACCCCTTTAGTTCTCTGGGTGTAGACCACACCACCTCCCACTGGG
  CACCCCCCAACACGGTGTCCTGCCACCCAGCGCCTGGCTCCAGGAAAACACGCTTGCCTT
  CCTTCCCGGCAGCTTCGCCACTCTCCTTATGGACTCTGTTCTGTTTGTACATGGCTGACG
  GAAATCTCTTTGGTACAACCGAATAAAGCCTGGTGGCAGTGCTGAAAAAAAAAAAAAAAA
  AA
• Restriction Sites: Please inquire
• ACCN: NM_002861
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002861.1, NP_002852.1
• RefSeq Size: 1856 bp
• RefSeq ORF: 1170 bp
• Locus ID: 5833
• UniProt ID: Q99447
• Cytogenetics: 17q25.3
• Domains: CTP_transf_2
• Protein Pathways: Glycerophospholipid metabolism, Metabolic pathways
• Gene Summary: This gene encodes an enzyme that catalyzes the formation of CDP-ethanolamine from CTP and phosphoethanolamine in the Kennedy pathway of phospholipid synthesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.