Bone marrow stromal cell antigen 1 (BST1) (NM_004334) Human Untagged Clone

CAT#: SC320381

BST1 (untagged)-Human bone marrow stromal cell antigen 1 (BST1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Bone marrow stromal cell antigen 1
• Synonyms: CD157
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004334.1 GGAACCGCCTTGGTAGAAGGAGAGAAGGGGAGTGGAGGAAGCACGGGACTGGAGGGACCA
  AAGTTCCCCGATGGCGGCCCAGGGGTGCGCGGCATCGCGGCTGCTCCAGCTGCTGCTGCA
  GCTTCTGCTTCTACTGTTGCTGCTGGCGGCGGGCGGGGCGCGCGCGCGGTGGCGCGGGGA
  GGGCACCAGCGCACACTTGCGGGACATCTTCCTGGGCCGCTGCGCCGAGTACCGCGCACT
  GCTGAGTCCCGAGCAGCGGAACAAGAACTGCACAGCCATCTGGGAAGCCTTTAAAGTGGC
  GCTGGACAAGGATCCCTGCTCCGTGCTCCCCTCAGACTATGACCTTTTTATTAACTTGTC
  CAGGCACTCTATTCCCAGAGATAAGTCCCTGTTCTGGGAAAATAGCCACCTCCTTGTTAA
  CAGCTTTGCAGACAACACCCGTCGTTTTATGCCCCTGAGCGATGTTCTGTATGGCAGGGT
  TGCAGATTTCTTGAGCTGGTGTCGACAGAAAAATGACTCTGGACTCGATTACCAATCCTG
  CCCTACATCAGAAGACTGTGAAAATAATCCTGTGGATTCCTTTTGGAAAAGGGCATCCAT
  CCAGTATTCCAAGGATAGTTCTGGGGTGATCCACGTCATGCTGAATGGTTCAGAGCCAAC
  AGGAGCCTATCCCATCAAAGGTTTTTTTGCAGATTATGAAATTCCAAACCTCCAGAAGGA
  AAAAATTACACGAATCGAGATCTGGGTTATGCATGAAATTGGGGGACCCAATGTGGAATC
  CTGCGGGGAAGGCAGCATGAAAGTCCTGGAAAAGAGGCTGAAGGACATGGGGTTCCAGTA
  CAGCTGTATTAATGATTACCGACCAGTGAAGCTCTTACAGTGCGTGGACCACAGCACCCA
  TCCTGACTGTGCCTTAAAGTCGGCAGCAGCCGCTACTCAAAGAAAAGCCCCAAGTCTTTA
  TACAGAACAAAGGGCGGGTCTTATCATTCCCCTCTTTCTGGTGCTGGCTTCCAGGACTCA
  ACTGTAACTGGAAACTGTGTTGCTCTAACCCTCCTCCAGCCCTGCAGCCTCCCCTTTGCA
  GTCATCATTCGTGTTCTGTGTATACCAAATGATTCTGTTATCTAAAGAAGCTTTTTGCTG
  GGAAAACGATGTCCTGAAAATGGTATTTCAATGAGGCATATGTTCAGGATTTCAGAAACA
  AGAAGTTAGTTCTATTTAGCAGGTTAAAAAATGCTGCATTAGAATTAAAGCAAGTTATTT
  TCTTATTTGTATAATGACACAAAGCATTGGGAGTCAGACTGCTTGTATATTATCAAACAT
  TTTAAGAGAATTCTAATAAAGCTGTATTTTACATCAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004334
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004334.1, NP_004325.1
• RefSeq Size: 1411 bp
• RefSeq ORF: 957 bp
• Locus ID: 683
• UniProt ID: Q10588
• Cytogenetics: 4p15.32
• Domains: Rib_hydrolayse
• Protein Families: Transmembrane
• Protein Pathways: Calcium signaling pathway, Metabolic pathways, Nicotinate and nicotinamide metabolism
• Gene Summary: Bone marrow stromal cell antigen-1 is a stromal cell line-derived glycosylphosphatidylinositol-anchored molecule that facilitates pre-B-cell growth. The deduced amino acid sequence exhibits 33% similarity with CD38. BST1 expression is enhanced in bone marrow stromal cell lines derived from patients with rheumatoid arthritis. The polyclonal B-cell abnormalities in rheumatoid arthritis may be, at least in part, attributed to BST1 overexpression in the stromal cell population. [provided by RefSeq, Jul 2008]