TOLLIP (NM_019009) Human Untagged Clone

CAT#: SC320186

TOLLIP (untagged)-Human toll interacting protein (TOLLIP)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TOLLIP
• Synonyms: IL-1RAcPIP
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_019009.2 GCTGACTGTGGCGGCGGCGGCCTCGAGGTGACAACTGTCTCCGTCGCAGGCTCCGGCGGG
  GGCGCAGGAGGTCGCCCGGCGCGTCACTGTCGGGTCGGCGAGCCACGGGGGCCGCCGCAG
  CACCATGGCGACCACCGTCAGCACTCAGCGCGGGCCGGTGTACATCGGTGAGCTCCCGCA
  GGACTTCCTCCGCATCACGCCCACACAGCAGCAGCGGCAGGTCCAGCTGGACGCCCAGGC
  GGCCCAGCAGCTGCAGTACGGAGGCGCAGTGGGCACCGTGGGCCGACTGAACATCACGGT
  GGTACAGGCAAAGTTGGCCAAGAATTACGGCATGACCCGCATGGACCCCTACTGCCGACT
  GCGCCTGGGCTACGCGGTGTACGAGACGCCCACGGCACACAATGGCGCCAAGAATCCCCG
  CTGGAATAAGGTCATCCACTGCACGGTGCCCCCAGGCGTGGACTCTTTCTATCTCGAGAT
  CTTCGATGAGAGAGCCTTCTCCATGGACGACCGCATTGCCTGGACCCACATCACCATCCC
  AGAGTCCCTGAGGCAGGGCAAGGTGGAGGACAAGTGGTACAGCCTGAGCGGGAGGCAGGG
  GGACGACAAGGAGGGCATGATCAACCTCGTCATGTCCTACGCGCTGCTTCCAGCTGCCAT
  GGTGATGCCACCCCAGCCCGTGGTCCTGATGCCAACAGTGTACCAGCAGGGCGTTGGCTA
  TGTGCCCATCACAGGGATGCCCGCTGTCTGTAGCCCCGGCATGGTGCCCGTGGCCCTGCC
  CCCGGCCGCCGTGAACGCCCAGCCCCGCTGTAGCGAGGAGGACCTGAAAGCCATCCAGGA
  CATGTTCCCCAACATGGACCAGGAGGTGATCCGCTCCGTGCTGGAAGCCCAGCGAGGGAA
  CAAGGATGCCGCCATCAACTCCCTGCTGCAGATGGGGGAGGAGCCATAGAGCCTCTGCCT
  CGATGCCGTTTTGCCCCCGCTCTTTGGACACGCCGACCCGGCGCTCCCCAAGGAATGCTG
  TCCCAACAAGATTCCCGTGAAAGAGCACCCGTGTCGCCCCCTCCCGTGGACTTCTGTGCC
  GCCCCGTCCACACCTGTTCTTGGGTGCATGTGGGTTTTCGGTTCCTGGCGGTCCAGGACG
  GGGCGGGGGCTCCCCTCCCATCTCGTGCTGGGAGGTCTCAGCGCGCTCTCCTGTCCCTGG
  GACGTGCGTCTCTCCTTCTCATGCCGTTCTGGAAAATGCTCTTGCTGTAGAGAGCAGCTG
  CTTCTGCCAGGGTGTTGGAGGTGGTGGAGCGCCTTCCGATTCCATTCATGGCATTTTGTG
  ATGTGATGTAATTGGAATAGAGCTGTTGATTTAAGGCACACACAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_019009
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019009.2, NP_061882.2
• RefSeq Size: 3615 bp
• RefSeq ORF: 825 bp
• Locus ID: 54472
• UniProt ID: Q9H0E2
• Cytogenetics: 11p15.5
• Domains: C2, CUE
• Protein Families: Druggable Genome
• Protein Pathways: Toll-like receptor signaling pathway
• Gene Summary: This gene encodes a ubiquitin-binding protein that interacts with several Toll-like receptor (TLR) signaling cascade components. The encoded protein regulates inflammatory signaling and is involved in interleukin-1 receptor trafficking and in the turnover of IL1R-associated kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1) encodes the longest isoform (1).